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Biomedical subjects

G Palka

Publications and source records attributed to G Palka.

At least 109 records · Page 6Linked to original sources
PubMed1987

On chromosomal DNA modifications by chemical and physical treatment of C-bands.

Experiments were performed on fixed metaphase chromosomes using standard techniques for revealing paracentromeric heterochromatin (C bands) followed by staining with acridine orange with the aim of studying C-banding mechanism. Data obtained suggest that the specific resistance to the chemical-physical treatments of the heterochromatic areas is a consequence of the particular structural conditions that the C-positive material shows only after its early renaturation.

Acridine Orange↗
PubMed1987

Cytogenetics and acute non lymphocytic leukemia.

The authors report haematologic and cytogenetic data from 47 patients with ANLL, demonstrating the usefulness of cytogenetic studies for the classification as well as for the prognosis of this disorder. Chromosome studies also permitted the classification of marrow cellularity in: all diploid metaphases (NN), diploid and aneuploid metaphases (AN), and all aneuploid metaphases (AA). The remission rate for patients in whom only normal metaphases were detected (NN patients) was 83% while the remission rates were 67% and 33% respectively for patients in whom both normal and abnormal metaphases were seen (AN patients) and for those in whom only abnormal metaphases were noted (AA patients). In all FAB subgroups, complete remission was related to chromosomal abnormalities, except for M4 patients who evidenced a large number of complete remissions, although presenting more chromosomal abnormalities. The longer survival in this subgroup may be related to rearrangements of chromosome 16, which is associated with a better prognosis.

Acute Disease↗
PubMed1986

Cytogenetics and bone marrow transplantation.

The authors report hematologic and cytogenetic data on 19 patients treated with allogeneic bone marrow transplantation (BMT) for severe hematologic disorders: 8 patients with chronic myelogenous leukemia, 6 with acute leukemia, 3 with severe aplastic anemia, 1 with refractory anemia, and 1 with beta-thalassemia major. Cytogenetic assays were performed on marrow cells before conditioning, 30 days after BMT, and at subsequent times. The authors discuss the role of cytogenetic studies in the evaluation of bone marrow engraftment, leukemic transformation of the graft, and disease relapse.

Acute Disease↗
PubMed1986

Ring chromosome 11. A case report and review of the literature.

A female infant with severe growth-weight retardation and with a ring chromosome 11, associated with trisomy X in 15% of metaphases, has been reported. A literature review of cases of r(11) shows that the clinical features of these patients, although showing different frequencies, are similar to those of the del(11q) syndrome. It has been suggested that the variability of the mental retardation in r(11) patients is attributable to the unstability of the ring and to the different break points in these two chromosomal rearrangements. The origin of the r(11) was also addressed by studying fragile sites of the parents at 11p15 and 11q25.

Abnormalities, Multiple↗
PubMed1984

[Adjuvant potential of vitamin E in the induction of experimental allergic encephalomyelitis: histological aspects].

In this study we report the effect of Vit. E, as an immunostimulating factor, on the induction of Experimental Allergic Encephalomyelitis in Lewis rat. The animals were inoculated intracutaneously in the plantar areas with emulsion of isologous spinal cord suspensed in Incomplete Freund's Adjuvant (IFI) and Vit. E. Histologic examination revealed the basic lesion of a perivenous cuff of mononuclear cells and small areas of demyelinated axons. The clinical signes are graded in order to the time of induction. It is possible an action of "adjuvanticity" of Vit. E on the various cells involved in the immune response by cell transformation and moltiplication.

Adjuvants, Immunologic↗
PubMed1984

[Clinical and cytogenetic staging of chronic myeloid leukemia. Philadelphia positive].

A cooperative study between clinical and cytogenetic steps in 44 patients with Ph'+ CGL is reported in order to verific the usefulness of the cytogenetic screening for the diagnosis and the right classification of the patients. The study of the clinical steps is carried out on the basis of the parameters suggested by Tura and coll.; in the one of the cytogenetic steps Sandberg classification modified by the Authors is adopted. In 40 cases the comparison shows a marrow correspondence between clinical and cytogenetic steps. In fact the overage survival in the classical true steps of the disease is almost the sance in the 1st and 2nd step. In the 3rd step the survival is strongly reduced meaning that the more chromosome alterations are observed the more survival is reduced.

Adolescent↗
PubMed1981

[Karyotype in chronic myeloid leukemia in a blastic crisis. I. Monosomy 16].

One case of Chronic Granulocytic Leukemia is reported, in which the patient showed, during a blastic crisis, an aneupolid cariotype 45, XX, t (9; 22) (q34; q11), -16. This paper emphasizes the rare involvement of the chromosome 16 in Leukemias, and stresses the high frequency of the alterations of the chromosome group E in the course of blastic crisis.

Chromosome Aberrations↗
PubMed1981

[Karyotype in chronic myeloid leukemia in a blastic crisis. II. Trisomy 17].

One case of a patient with Chronic Granulocytic Leukemia showing a double Ph' together with trisomy 17 during blastic crisis is reported. The Ph' chromosome resulting from a standard translocation of the chromosomes 9 and 22 was present in all the 16 mitoses observed, while the trisomy 17 was found in 15. This case is an additional contribution which demonstrates the presence of the alterations of the chromosome group E during the blastic crisis.

Chromosomes, Human, 16-18↗
PubMed1981

[Karyotype analysis during development of chronic myelocytic leukemia].

We report cytogenetic data concerning 9 patients with chronica granulocytic leukemia and eosinophilia at the onset and during the chronic phase of the disease before the blastic crisis. The cariotype analises in the intermediate phases allowed to determine the first changes which could be involved in the evolutionary events of the disease up to the moment in which the typical markers of the blastic crisis can be found.

Adult↗
PubMed1981

[Cytogenetics in bone marrow transplantations. I. Bone marrow aplasia].

A bone marrow transplantation has been carried out in a patient with bone marrow aplasia. Besides the cytochemical and haematological tests, the cytogenetic analysis has been performed to check whether the transplantation was successful. In this case the donor was the patient's sister, so that the presence of the chimere has been used as a criteria for judging the conditions of the transplantation. the cytogenetic analysis, furthermore, is suitable to detect other chromosome abnormalities, which can represent a condition of instability of the transplanted cells and are probably an early expression of the transplanted bone marrow.

Adolescent↗
PubMed1981

[Cytogenetics in bone marrow transplantations. II. Acute lymphocytic leukemia].

In this we report cytogenetic data concerning two patients with Acute Lymphoblastic Leukemia (ALL), submitted to bone marrow transplantation. In one of two patients the chimere was present while in the other case it was absent, since the donor and the acceptor were of the same sex. However even in the latter case, the cytogenetic analysis was useful and led to the identification of endomitosis and endoreduplication phenomena, which are signs for an unlucky prognosis.

Bone Marrow Transplantation↗
PubMed1981

Interactions among DNA, metallic ions, and lipids.

Interactions amond DNA, phospholipids, and Cu2+ ions have been investigated by means of thermal denaturation technique. The results indicate that phosphatidylserine and sphingomyelin interact with Cu2+ ions, which are able to prevent the chance of binding of the phospholipids to the double helix. This interaction is strictly dependent upon the concentration of the divalent cation and reduces the capability of the employed phospholipids to modify the thermal stability of DNA.

Chemical Phenomena↗
PubMed1980

[Karyologic analysis in erythroleukemia].

We report the cytogenetic data of two patients with erythroleukemia showing the chromosome Ph'. In one case the chromosome Ph', as revealed with the GTG band technique was the result of a translocation involving the chromosomes 19 and 22. The aim of this work is to provide more contribution to the knowledge of the origin of the Ph' and to give data in the field of this disease for which few cytogenetic data are available after the introduction of the banding techniques.

Aged↗