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Biomedical subjects

G Petri

Publications and source records attributed to G Petri.

At least 19 recordsLinked to original sources
PubMed2000

Free radical scavenging and membrane protective effects of methanol extracts from Anthriscus cerefolium L. (Hoffm.) and Petroselinum crispum(Mill.) nym. ex A.W. Hill.

The antilipoperoxidant activity of Anthriscus cerefolium L. (Hoffm.), chervil, Petroselinum crispum (Mill.) Nym. ex A. W. Hill., parsley extracts were evaluated with ascorbic acid induced lipid peroxidation on rat brain homogenates. These results are completed by the antiradical potential of these extracts against a solution of OH. radical. In all cases luteolin-7-O-glucoside was used as a reference material.

Animals↗
PubMed2000

In vitro antioxidant activity of Anthriscus cerefolium L. (Hoffm.) extracts.

Standardised aqueous extracts of chervil (Anthriscus cerefolium L. Hoffm.) (Apiacae) were investigated for antioxidant effect. Numerous in vitro test methods were used to determine whether the extracts, from different vegetative parts (root, herb) had H-donor, metal binding, reductive, free radical scavenging and membrane protective activity. Apiin was used as a reference material. The herb extract showed better activity in all experiments than the root extract. The present results underline that the wateric chervil extracts have antioxidant and anti-lipoperoxidant activity.

Animals↗
PubMed1998

[Investigation of the in vitro antioxidant effect of Petroselinum crispum (Mill.) Nym. ex A. W. Hill].

Free radical reactions have excited excessive research in the past two decades. Since then it has been proven that these mechanisms may be important in the pathogenesis of certain diseases and aging. Many synthetic antioxidant components have shown toxic and/or mutagenic effects, which have directed most of the attention on the naturally occurring antioxidants. Their use has mainly centered around prevention, and the maintenance of health. Parsley, Petroselinum crispum (Mill.) Nym. ex A. W. Hill belonging to the Apiaceae family, is a well-known spice and vegetable. Its herb and root are widely known for their effects on digestion, stomach, kidney, blood, and liver. The essential oil obtained from the fruit has also strong action on the central nervous system. Characteristic constituents are: flavonoids (apiin, luteolin-, apigenin-glycosides), essential oil (apiol, miriszticin), cumarines, (bergapten, imperatorin) and vitamin C. In our experimental work, various extracts prepared from different vegetative organs of parsley have been investigated. The chemical composition of the extracts and fractions were analyzed by chromatographic (GC, HPLC) and spectroscopic (UV, UV-VIS) techniques. We intended to provide evidence for the antioxidant activity of vegetable drugs and also studied the free radical scavenger activities by means of spectrophotometry (H-donor activity, reducing capability, chelat formation) and chemiluminometry. To prove the free radical scavenging effect of the extracts, the reduced intensity of the H2O2/.OH-isoluminol, microperoxidase system was studied in vitro. The highest correlation was found between the chemical property and the antioxidant effect of the flavonoid rich samples. At the same time it was also observed that the essential oil plays a significant role in the scavenging effect as well. More experiments are in progress to study the most promising compounds in the vegetative and generative organs of parsley.

Antioxidants↗
PubMed1998

[Determination of 23 elements in lady's thistle (Silybum marianum L. Gaertner)].

23 elements (Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, P, Pb, S, Ti, V, Zn) were measured in lady's thistle (Silybum marianum L.) herba and fructus by ICP-AES technique after digestion in teflon vessels. This is the first report about the content of these elements in the plant originated from Hungary. This measurements is important in view of plant physiology and pharmacology. Significant amounts of aluminium and iron were measured in lady's thistle herba. In the course of tea making, the relative concentration of some elements decreases because of the poor solubility of the compounds of these elements. The low concentration of these elements in teas in favourable in pharmacological aspect. No significant differences were found in boron, calcium, potassium and magnesium concentrations of lady's thistle fructus samples of different origin.

Boron↗
PubMed1995

[Gas chromatographic analysis of cannabinoids on tandem columns].

A simple, isotherm packed column gas chromatographic method was developed for the quantitative determination of neutral cannabinoids using 4-androstene-3,17-dion as internal standard. In order to achieve the best resolution and to avoid the evaluation of the disturbing hydrocarbon peaks a method was developed using "tandem" column made of 3% OV-1 (90%) and 3% OV-17 (10%) stationary phases. The psychotropic cannabinoids delta 1-tetrahydrocannabinol (delta 1-THC) and delta 1(6)-tetrahydrocannabionol (delta 1(6)-THC), as well as, their main metabolites cannabidiol (CBD) and cannabichromene (CBC) were baseline separated except the cannabigerol (CBG) and cannabinol (CBN) pair, however for these compounds the separation was also satisfactory for the quantitative determinations. The Kováts retention indices were calculated for the most important cannabinoids and established the detection limits, respectively (20-50 ng range). The reproducibility was found excellent cv% = 1.06 for delta 1-THC and the analysis time was 55 minutes. The practical usefulness of the method was demonstrated by the comparative analyses on hashish- and fibre type hemps.

Androstenedione↗
PubMed1994

[Instrumental analysis of medicinal plants and their drug products].

The experiences obtained during the development of gas chromatographic and other (GC, TLC and infrared spectrophotometric) methods for the 7th edition of the Hungarian Pharmacopoeia for essential oils and drugs containing essential oils are summarized with emphasis on the selection of suitable internal standard for the gas chromatographic assays. The qualitative and quantitative estimation of bitter compounds and polyphenoles e.g. flavonoids and procyanidines by means of ultraviolet and infrared spectrophotometry and HPLC is also described. Some HPLC methods for the determination of anthocyan and carotinoid derivatives are also presented. These are not yet included in the pharmacopoeia but are successfully used for the analytical investigation of commercially available medicinal plants and drug products made thereof.

Chromatography, Gas↗
PubMed1994

[Spectrophotometric and chromatographic investigation of bilberry anthocyanins for qualification purposes].

Analysis of the bilberry anthocyanin extracts of different origin (French, Polish, Roumanian and Italian) was performed for qualification and identification. Direct spectrophotometry was considered as simple, informative method for controlling the purity of the extracts and proving the presence of polyphenols with the estimation of the ratio A1 cm1% 535/A1 cm1% 280. Thin-layer chromatography is an appropriate technique for the separation of 5 to 6 constituents in the extracts for qualification and for the evaluation of compound-ratios by TLC-densitometry. The new RP-HPLC gradient procedure seemed to be the best method for the standardization of bilberry extracts by the separation and quantitative estimation of 12 anthocyanins. Therefore it is of great value in the standardization of anthocyanin extracts and crude drugs containing them.

Anthocyanins↗
PubMed1993

Influence of phaseolotoxin on the alkaloid accumulation in Datura innoxia Mill.

In crude protein extracts of aseptically grown plantlets of Datura innoxia ornithine carbamoyltransferase was detected. The ornithine carbamoyltransferase of plant extracts was inhibited by phaseolotoxin. The level of this inhibition was dependent on the concentration of the toxin applied. Subsequent accumulation of ornithine in plant vegetative parts was observed. Phaseolotoxin influenced both the vitability and alkaloid accumulation of treated plants.

Alkaloids↗
PubMed1992

Inhibition of aluminium toxicity by magnesium and citric acid in tobacco tissue culture.

Using tobacco callus tissues the authors studied the antagonism of magnesium and aluminium, and the inhibition of aluminium toxicity by magnesium and citric acid, as expressed by their effect on growth and alkalod content. In response to an increase in the concentration of MgSO4 in the culture medium the fresh weight of the cultures significantly increased, while with an increase in Al2(SO4)3 growth decreased, 2 mM causing a high degree of inhibition. The toxic effect of aluminium was less marked when Mg was present at a concentration higher by an order (antagonistic inhibition). When citric acid was added to the culture medium, promoting complex formation with Al, the toxic effect of Al was not apparent. The nicotine content of the tissues was in each case inversely correlated with the magnitude of biomass formation.

Alkaloids↗
PubMed1990

[Quality control of essential oil of muscat sage (Salvia sclarea) during laboratory and industrial preparation. IV. Causes of color changes].

Essential oils of muscat sage--of various habitats in different developmental stages as well as the essential oils prepared with half industrial and laboratorial procedures--have been studied. It has been found that compositions of essential oils do not differ significantly, the difference is only in such an extent that could be expected on the ground of environmental and ontogenetic effects. On the basis of tests it has been established that formation of brown oil is in connection with content of copper of apparatus (boiler, tube); Therefore applications of vessel and connections containing copper or copper impurities must be avoided in preparation of the clear essential oil.

Color↗
PubMed1990

[A case of meningococcal sepsis].

We describe a 7-years boy by with a meningococcal sepsis due to Neisseria Meningitidis, with very serious evolution of cutaneous necrosis, initial D.I.C. and heart failure. The clinical picture do not improve with antibiotic therapy (CAF-penicillin), but the association ceftriaxone + tobramycin results in rapid improvement. The cutaneous necrosis, especially evident on gluteus, arms and legs, were treated locally with AgNO3 and penicillin-solution. After 4 weeks of treatment, also this cutaneous involvement improved and now the boy is healthy, without residual signs neither systemic nor cutaneous.

Acute Disease↗
PubMed1988

Histochemical detection of hemp trichomes and their correlation with the THC content.

Authors investigated some fiber and some hashish hemp sorts concerning their trichomes on the leaves. Histochemical reactions were developed using the Fast Blue Salt (FBB) reagent applied so far only thin layer chromatography. Glandular hairs were found giving positive reactions due to cannabinoids contained by the cells. The electron microscopic features were studied and the cannabinoid content was measured with GC. A correlation was found between the number of typical glandular hairs and cannabinoid content.

Cannabinoids↗
PubMed1987

Plasma catecholamine levels in the postoperative period in complication-free and "paralytic" ileus patients.

Plasma catecholamine concentrations were compared in a group of postoperative "paralytic" ileus patients and in another group of patients, who had undergone medium-size abdominal operations followed by uneventful recovery. The plasma epinephrine level was significantly in the former group, whereas no such difference was observed in the norepinephrine concentration. The data appear to confirm that the epinephrine released from the adrenal medulla appreciably contributes to the development of "paralytic" ileus. The therapeutically effective major tranquillizer and alpha-receptor blocking drug, trifluperidol, was found to reduce both epinephrine and norepinephrine levels in "paralytic" ileus patients. The decrease of the plasma epinephrine level was the higher, the greater its initial concentration. These findings seem to support the decisive role of increased catecholamine release in the development of postoperative motor inhibition ("postoperative" ileus) and also explain the success of sympatholytic treatment in such cases, i.e. the return of normal peristalsis.

Abdomen↗
PubMed1986

Changes of small bowel motility and noradrenaline content of the intestinal wall in response to alpha- and beta-adrenergic blockade in dog.

In experiments on dogs, the spontaneous movements of the small bowel were in all cases enhanced by the alpha 2-blocker phentolamine, while they were not influenced, or were slightly decreased, by the beta 1-blocker practolol. Neither drug caused a change in the noradrenaline content of the intestinal wall. In the same animals, the joint administration of phentolamine and practolol led to a considerable increased small bowel motility, and to a significant decrease in the noradrenaline level of the intestinal wall. The results are in agreement with experimental data indicating that presynaptic alpha-receptors play a primary role in the sympathetic regulation of small bowel motility.

Animals↗
PubMed1985

[Our surgical heritage: the tragic destiny of the surgeon. Eugen Alexander Pólya (1876-1944)].

Eugen Pólya was an outstanding hungarian surgeon of the first half of this century, a man of international reputation. Already in 1913 on the basis of animal experiments he realised the necessity of conservative treatment in acute pancreatitis. He and Reichel (Germany) share the fame of the introduction of a modified GE in partial resection of the stomach (B-II-type). In 1944 Pólya was killed by hungarian fascists for racial and political reasons. Nobody knows where his corpse was buried.

General Surgery↗