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Biomedical subjects

G Pfliegler

Publications and source records attributed to G Pfliegler.

51 records · Page 3Linked to original sources
PubMed1985

Aggristin (ristomycin) precipitation test: a new tool for the detection of fibrin monomer and fibrin degradation products.

The specific detection of fibrin monomer and fibrin degradation products is of high importance in the laboratory diagnosis of intravascular clotting (disseminated intravascular coagulation, deep vein thrombosis). The methods proposed until now are partly time-consuming, needing special laboratories or insensitive and poorly specific. Applying ristomycin instead of ristocetin (another member of the vancomycin antibiotics) a new simple, specific and sensitive method has been elaborated and recommended for the laboratory diagnosis of intravascular coagulation and its differentiation from primary fibrinogenolysis. The results obtained from in vitro and animal experiments and from human studies are presented.

Animals↗
PubMed1984

Detection of platelet-associated IgG in chronic immune thrombocytopenic purpura using antibody-coated polyacrylamide beads.

Platelet-associated IgG (PAIgG) was detected by means of anti-human IgG coated polyacrylamide beads ("Immunobeads") technique in 32 patients with chronic ITP. Both a direct test (with in vivo sensitized platelets) and an indirect test (with in vitro loaded platelets) were carried out. The percent of rosette forming beads was both in the direct test (41.2%) and in the indirect test (32.6%) significantly higher in the cases of chronic ITP patients than in the controls (2.5% and 3.2%, respectively). These results confirm the diagnostic value of this new, relatively simple and rapid method in routine clinical practice.

Acrylic Resins↗
PubMed1984

The effect of external sodium on ouabain-insensitive K influx in fresh human red blood cells.

The rate of 42K influx was investigated at various external sodium and ouabain concentrations in human red blood cells. In agreement with earlier reports, in red blood cells not treated with ouabain, Na did not affect K influx when [K]0 was 5.0 mM while it reduced the influx at [K]0 = 0.15 mM. However, Na stimulated 42K influx at both 5.0 mM and 0.15 mM in cells treated with ouabain (1 X 10(-5) M). When external Na concentration was raised from 0 to 72 mM the rate of 42K influx increased at [K]0 = 0.15 mM and at ouabain = 1 X 10(-5) M. The effect of external Na at different ouabain concentrations showed that K influx was inhibited by Na without or with ouabain in less than 5 X 10(-6) M while an increased K influx could be observed with higher ouabain concentrations in the incubation media. The results suggest that in the case of the complete inhibition of ouabain-sensitive K influx the electrochemical gradient of the Na ions may serve as a driving force for the inward movement of potassium.

Biological Transport, Active↗
PubMed1983

Plasma levels of beta-thromboglobulin and factor VIII-related antigen in diabetic children and adults.

In order to investigate the relationship between the in vivo platelet activation in diabetes mellitus and the endothelial damage connected with the diabetic micro- and/or macroangiopathy, plasma levels of beta-thromboglobulin (B-TG) and of factor VIII-related antigen (VIII R:Ag) were studied (1) in juvenile-onset (Type I) diabetics without clinical signs of angiopathy (age under 12 years) and (2) in mostly maturity-onset (Type II) diabetics with and without overt angiopathy (age between 14 and 60 years). Normal controls and nondiabetics with atherosclerosis were also studied. Plasma levels of both proteins were found to be elevated in all the groups of diabetic and atherosclerotic patients in comparison with the controls. Highest levels were found in adult diabetics with angiopathy and in atherosclerotics even without diabetes, but values of the diabetic children were also elevated. The data suggest a causal relationship between the vascular damage and the enhanced platelet reactivity in which the former may play the primary role.

Adolescent↗
PubMed1983

The influence of catecholamines on Na, K transport in slow- and fast-twitch muscles of the rat.

The effects of catecholamines on active sodium and potassium transport was compared in slow- (SOL) and fast-twitch (EDL) skeletal muscles of the rat. Stimulation of active Na+-extrusion and K+-uptake induced by adrenaline (6-30 mumol . l-1) and isoprenaline (1-40 mumol . l-1) was markedly greater in slow- than in fast-twitch muscle. In sodium-preloaded muscles the maximal stimulation of 24Na-efflux induced by adrenaline was about 2-fold higher in SOL than in EDL. Isoprenaline caused a 2.4-fold increase in ouabain-sensitive 86Rb influx in SOL muscle, but failed to alter the ouabain-sensitive influx in EDL. The stimulating action of isoprenaline on 86Rb influx in EDL was due to an increase in the ouabain-insensitive fraction of Rb uptake. The effects of catecholamines of fast- and slow-twitch muscles were probably due to the accumulation of cyclic AMP, however the fact that there were no significant differences between the nucleotides levels in fast- and slow-twitch muscle suggests the participation of other mechanism as well. The results presented suggest that cyclic AMP-induced stimulation of ouabain-insensitive transport of cation in the isolated EDL muscle of the rat is similar to that of barnacle muscle.

Animals↗
PubMed1981

The subcellular location of potassium flux pathways in frog skeletal muscle.

The influence of ouabain and physostigmine on 42K and 86Rb uptake in isolated from sartorii with normal [Na]i(12-14 mmol.kg -1 wet weight) and low [Na]i (6 mmol.kg-1 wet weight) was compared. Both in normal sodium and in low sodium muscles application of 10-3 M physostigmine reduces potassium influx by about 70%. About one forth of potassium-uptake in normal-sodium muscles is inhibited by ouabain (10-4 M) and only a very slight fraction of potassium uptake is ouabain-sensitive in low-sodium muscle. The effects of ouabain and physostigmine on 42K influx are additive. The greater parts of the Rb-fluxes are through the ouabain-sensitive pathway. Glycerol treatment has no effect on ouabain-sensitive channels although it inhibits markedly the K-flux through the physostigmine-sensitive pathway. The results suggest that the Na-K-ATPase is located in the surface membrane while most of the physostigmine-sensitive K-exchange is within the tubules.

Animals↗
PubMed1981

Congenital deficiency of cyclo-oxygenase in a woman with generalized atherosclerosis.

The case of a 52-year-old woman with congenital cyclo-oxygenase deficiency, signs of generalized atherosclerosis and a moderate bleeding tendency is reported. Secondary platelet aggregation was absent. Platelet aggregation induced by arachidonic acid failed totally while that induced by calcium ionophore was normal. No malondialdehyde formation could be detected in her platelet-rich-plasma. The life-long deficiency of cyclo-oxygenase had not protected her from progressive vascular disease. This case suggests that the chronic intake of large doses of aspirin cannot prevent arterial disorders.

Arteriosclerosis↗
PubMed1981

The inhibitory actions of eserine and ouabain on the K, Rb and Cs uptake in slow and fast twitch muscles of the rat.

Comparative, in vitro studies were carried out on the 42K, 86Rb, and 131Cs uptake in fast twitch extensor digitorum longus (EDL), slow twitch soleus (SOL) muscles of the rat, and in fast muscle (sartorius) of the frog. The inhibitory action of ouabain (10(-4) M) and eserine (10(-3) M) on the influxes of alkali cations was investigated. The rate of potassium influx in isolated EDL muscles was higher than that of SOL, while no difference could be found in 86Rb or 131Cs influx in the two types of mammalian muscles under in vitro condition. Ouabain inhibited to about the same extent the influx of K (25%) of both types of mammalian muscles and also in fast amphibian muscle. On the other hand, the eserine sensitive component of 42K influx in fast twitch mammalian muscle (EDL) was about one-fourth of the total influx and even less in slow twitch mammalian muscle (SOL), while in frog muscle it amounted to about two-third of the total. The "residual" potassium influx, which represents the influx remaining after simultaneous treatment of the muscles with cardiac glycoside and eserine was about half of the total in EDL and SOL, but it was only a fraction of it in the frog sartorius muscle. The results may be explained on the basis of the morphological differences of the transverse tubular and sarcoplasmic reticulum systems of fast and slow mammalian muscles.

Animals↗