Ovarian steroidogenesis studied by mass fragmentography.
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Biomedical subjects
Publications and source records attributed to G Phillipou.
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The preparation of allyldimethysilyl steroid ethers for the analysis of steroids by GC-MS has been studied in depth. The application of these derivatives is shown to be unsatisfactory due to competitive formation of allylsiloxane derivatives.
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Plasma phytanic acid has been quantitated by mass fragmentography after its successive reduction and derivatization to the corresponding t-butyldimethylsilyl ether. The latter was estimated by selective ion monitoring of its characteristic (M-57) ion (M/z 355). The technique has the advantage of being more rapid, specific and sensitive than existing methods, permitting the determination of phytanic acid at levels greater than 5 mug/ml plasma.
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The mass spectra of t-butyldimethylsilyl fatty acid esters all display a pronounced (M-C4H9)+ ion. The proportion of the total ionization carried by this fragment, particularly for saturated and mono-, di-, and tri-unsaturated acid derivatives, facilitates their qualitative analysis at the subanogram level by mass fragmentography.
A simple preparation of androsta-2,4-dien-17beta-ol from testosteroneis described...
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A quality control (QC) programme to continually monitor the reliability of hospital ward capillary blood glucose levels has been developed based on a memory blood glucose meter interfaced to a portable microcomputer. Over a 38-week period some 12,500 ward QC tests were performed by approximately 200 accredited operators. Although the mean weekly coefficients of variation were all less than 15%, there was a marked variation between wards, with the frequency of testing of patients the major factor influencing reliability. However an external laboratory-based QC programme suggested a high degree of proficiency which was not achieved by any ward on internal QC assessment.
The relationship between the 1-h 50-g glucose challenge performed at 24-28 weeks and subsequent birth of a large-for-gestational-age infant was examined in a cohort of 368 Europid women who had no pre-existing medical condition and experienced no pregnancy complications. The results established that the 1-h 50-g glucose challenge blood glucose level had no predictive power with respect to the birth of a large-for-gestational-age infant. However, the 1-h blood glucose value was found to be significantly related to maternal height, weight and age, indicating that its level also represents processes unconnected to glucose intolerance. The study findings also re-confirmed the importance of parity, height, sex, and weight as determinants of large-for-gestational-age infants.