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Biomedical subjects

G Picerno

Publications and source records attributed to G Picerno.

14 recordsLinked to original sources
PubMed1988

Importance of some factors on the dimorphism of Candida albicans.

The passage between the yeast and mycelial forms of Candida albicans B 311-10 was studied by using the minimal synthetic medium of Shepherd et al. modified without biotin and with low glucose concentrations. It was observed that biotin, aminoacids and particularly pH are not important factors in the dimorphism of C. albicans. The only factor of notable importance in the passage of yeast form to mycelial form in C. albicans was glucose concentration.

Amino Acids↗
PubMed1988

A synthetic medium for Saccharomyces cerevisiae.

Growth of Saccharomyces cerevisiae strain Castelli 20 has been observed in a minimal synthetic medium using different glucose concentrations and without biotin, during the first 30 hrs of its development at 28 degrees C. The yeast's growth was observed spectrophotometrically at 675 nm reading the Optical Density every hour. The minimal medium of Sheperd et al. (1980), with glucose 15 g/L and biotin was modified: the vitamin was eliminated and the concentration of glucose was gradually reduced down to 0.5 g/L. At 5 g/L of glucose concentration and without vitamins the strain grew very well. From our results up to 30 hrs biotin has no influence on the yeast's growth. The medium would be useful to study Saccharomyces cerevisiae physiology during the early period of its development.

Biotin↗
PubMed1985

[The Autobac MTS system].

The Autobac MTS is a semiautomatic modular system for the screening of urine cultures and the execution of both antibiogram and MIC. The principle on which these tests are based is that of turbidimetry. In this note, the authors examine in detail the determination of MIC, that is based on utilisation of the regression lines equation. The method used by Autobac has a greater precision than the break-point method. This fact is mostly due to the possibility of standardizing inoculum. The authors conclude that, on the ground of their experience, the MIC method always some disagreement with the literature for the tested antibiotics (aminoglycosides).

Aminoglycosides↗
PubMed1983

[Analysis of viral capsid anti-antigen antibodies of the Epstein Barr virus in infectious mononucleosis. Comparative evaluation of a fluorescence method with classic methods].

708 sera were tested by a FA method for the detection of antibodies anti-viral capsid antigen in Epstein-Barr virus disorders to establish its effective value in infectious mononucleosis diagnosis. The arise of IgG class's antibody was not significative between recent infectious mononucleosis's patients and controls. Preliminary study of a test to research IgM anti-viral capsid antigen confirmed its value in infectious mononucleosis's diagnosis, but only in early stages of infection. At least, after evaluation of the results obtained by FA test for IgG-anti-viral capsid antigen in 200 blood donors the authors suggest the utilization of this method as screening in these people to avoid infectious mononucleosis transmission by blood transfusion in newborn children or in immunosuppressed patients.

Adolescent↗
PubMed1979

[The post-transfusional toxoplasmosis (author's transl)].

The sera of 85 patients suffering from Cooley anemia, 100 normal children of the same group of age, and 550 blood donors have been tested for the presence of anti-toxoplasma antibodies by indirect immunofluorescence test. The sera of 10 thalassemic patients gave positive reactions at a dilution reaging from 1/64 to 1/2048; in 2 sera a positive reaction was obtained with specific anti-IgM antibodies, indicating an active infection. In the control group, only 2 positive sera have been found. In donors group, instead, 5 sera were positive, and in 2 cases IgM antibodies were detectable. The analysis of data confirms, although indirectly, that heavily transfused patients are at a particular risk to acquire the infection from Toxoplasma Gondii.

Adolescent↗
PubMed1978

[Research on the eventual cross-reactivity of anti-Wr(a) with various viral, bacterial and mycotic antigenes (author's transl)].

Among the sera of 1011 blood donors, they have been collected 34 anti-Wr(a) antibodies. By IgG antiglobulin test, the titer was 1/8 or more in 21 sera. After absorption on viral, bacterial and mycotic antigens, the sera were still reactive with Wr(a) + red blood cells. These results show that no tested antigen is cross-reactive with Wr(a) antigen. However, the AA. suggest that the research of a widley diffused antigen, cross-reactive with Wr(a) + red blood cells, is a valuable approach to the problem of IgG anti-Wr(a) antibodies in normal, never transfused blood donors.

Adult↗
PubMed1975

[Value of TPHA in the serological screening of lues].

In the present work, 21,400 not selected sera were tested with hemagglutination test (TPHA) and flocculation test (VDRL) with cardiolipin for screening of syphilis; sera that gave negative results against both tests showed remarkable agreement (97,81%). 468 sera gave positive results to least one of the tests employed in screening and were tested with the following procedures: fluorescent Treponemal antibody-absorption (FTA/ABS) test, complement fixation (by Kolmer, modified) with proteic (KP) and lipidic (KL) antigen; 100 of these sera were also tested with T. pallidum immobilizaiton (TPI) test. The agreement among the before mentioned tests and sera that gave positive results only to VDRL was significatively low; on the contrary, the agreement was remarkable in the case of those positive only to TPHA (99% with FTA/ABS, 83% with TPI, 52,5% with KP and 21,5% with KL). The results obtained in attesting sensitivity and specificity of TPHA indicate that it is a very valuable screening test, and suggest to the AA. to emphasize that TPHA/VDRL association a definitive conclusion in syphilis serological negativity.

Antibody Formation↗
PubMedDate not supplied

[Serological survey for virus and Mycoplasma infections (author's transl)].

The Authors describe the two years follow up of complement fixing antibodies vs 4 viral antigens (Herpes simplex, Herpes zoster, Cytomegalovirus, respiratory syncytial virus, Mycoplasma pneumoniae). They evaluate the seasonal trend and the eventual clinical correlations on 3267 sera and 254 spinal fluids from hospitalized patients.

Antibodies, Bacterial↗
PubMedDate not supplied

[Diagnostic patterns of CSF immune response (author's transl)].

The AA. critically evaluate the different methods used for the study of the CSF proteins and particularly examine the diagnostic meaning of some blood and CSF proteins ratios. According to the cases they report, the AA. think that some patterns characteristic of the nervous system diseases can be defined and they point out the importance of the contemporaneity of the examination of the CSF and of the serum in order to improve the significance of laboratory data.

Bacterial Infections↗