[Somatic growth and psychomotor development of the premature infant in the first 3 years of life. Influence of social factors studies by a domicilliary survey].
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Biomedical subjects
Publications and source records attributed to G Pilotti.
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Two cases of purulent meningitis by Group B streptococci are reported. The bacteria agents were clearly transmitted transplacentally from mother to child. The bacteriological and clinical data are reported and discussed in relation to international literature.
The authors investigated and compared lymphocyte subsets natural killer in 33 newborns and in 33 healthy adults. From 5 ml of heparinized venous blood were separated mononuclear cells on density-gradient Ficoll-Hypaque. The vitality was determined with immunofluorescent method (orange acridine and ethiobromide). It was positive if resulted at least 95%. Lymphocyte phenotype was also examined after incubation with monoclonal antiserum Becton-Dickinson conjugated with FITC and PE for 45' at 4 degrees C. Two hundred cells were counted on each slide with fluorescent microscopy. Total number of T-lymphocyte (Leu-4) was increased in newborns; total B-cells (Leu-12) were similar in newborns and healthy adults; T-helper/T-suppressor (Leu-3a/Leu-2a) ratio was above the normal range for increase T-helper. The percentage of subsets with double marker Leu-2a+/Leu-3a+ was always about 15-20%. NK cells with phenotype HNK-1 (Leu-7) were a little number in newborns while the percentage of NK-cells-Leu-11a was similar among adults and newborns.
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