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Biomedical subjects

G Pott

Publications and source records attributed to G Pott.

At least 55 records · Page 3Linked to original sources
PubMed1980

Diagnostic significance of connective tissue enzymes.

In spite of the extensive literature, little is known of the diagnostic value of the enzymes of connective tissue metabolism. This review summarizes the available information and assesses the significance of enzyme measurements for the diagnosis of hereditary and nonhereditary connective tissue diseases, with emphasis being given to the roles of abnormal activities of collagenases and lysosomal glycosidases in the detection of fibrotic processes.

Acetylglucosaminidase↗
PubMed1980

Rapid determination of fibronectin by laser nephelometry. Fibronectin concentrations is plasma in human diseases, I.

Fibronectin (Synonyms: cold-insoluble globulin, antigelatin factor) is a cell surface glycoprotein. It has been shown that remarkably large amounts of fibronectin are present in human plasma. Owing to the difficult and expensive radioimmunoassays, only a few reports on the relation of fibronectin and human diseases have so far been published. In this communication a rapid and easily handled immunoassay, using the light scattering effect, is described. The first results on the measurement of fibronectin concentrations in the plasma of patients with chronic active liver diseases are reported.

Antibodies↗
PubMed1980

Activity of isoenzyme A and N-acetyl-beta-glucosaminidase in fibrotic liver tissue.

Isoenzymes of N-acetyl-beta-glucosaminidase were separated in the supernatant of 72 homogenized normal and fibrotic human liver. Patterns of isoenzymes were obtained by electrofocusing in thin layers of polyacrylamide gel. The activity of mesenchymal reaction in the liver tissue was determined by histological examination. The distribution of N-acetyl-beta-glucosaminidase isoenzymes was compared with the mesenchymal activity in the liver tissue. The results show a good correlation of isoenzyme A activity with mesenchymal activity.

Acetylglucosaminidase↗
PubMed1979

Activity of procollagen-prolyl-hydroxylase and N-acetyl-beta-glucosaminidase in liver biopsies from patients with chronic liver diseases.

In 5 mg of liver tissue obtained by needle biopsy from patients with chronic liver diseases, activity of both Procollagen-prolyl-hydroxylase and N-Acetyl-beta-glucosaminidase was measured. The activity of both enzymes increased due to the activity of fibrosis, but prolylhydroxylase increased more. The relation of these two enzyme activities in the development of liver fibrosis is discussed.

Acetylglucosaminidase↗
PubMed1979

Influence of somatostatin on carbohydrate absorption in human small intestine.

Oral carbohydrate tolerance tests with xylose, galactose and lactose were performed. After an interval of at least 24 h the same tests were repeated following an i.v. bolus and during infusion of somatostatin. Somatostatin does not influence xylose absorption. However, absorption of galactose and lactose is significantly reduced (p less than 0.01/0.008) during somatostatin infusion. On the other hand, serum levels of galactose remain unchanged despite administration of somatostatin, when galactose is given parenterally. The results support the assumption that the absorption process in small intestine is affected by somatostatin. Possible effects of somatostatin on hormones regulating the intestinal absorption and on energy-depending carrier mechanisms are discussed.

Adult↗
PubMed1979

[Correlation of morphometric parameters with enzyme-activities in cases of liver diseases with fibrosis (author's transl)].

50 liver specimens with fibrotic liver disease were taken from current autopsy material and, after preparation, subjected to automatized image analysis by QUANTIMET 720 for quantitation of the connective tissues and their cellularity. In cell-free supernatants of liver tissue homogenates we determined the activities of N-acetyl-beta-glucosaminidase (beta-NAG, E.C. 3.2.1.30), a lysosomal glycosidase that is involved in proteoglycane metabolism of connective tissue. Beta-NAG-isoenzymes were demonstrated by electrofocusing in thin layers of polyamide gel. The results showed statistically significant correlations between beta-NAG activity and morphometrically assessed quantities of connective tissue, and of mesenchymal cells found in the latter or in the whole specimen, respectively. Demonstration of tissue or serum levels of beta-NAG or its isoenzymes provides a readily practicable method for assessing mesenchymal activities of the liver.

Acetylglucosaminidase↗
PubMed1978

[Isoenzyme P of N-acetyl-beta-glucosaminidase in sera from patients with liver diseases (author's transl)].

The isoenzyme P of N-Acetyl-beta-Glucosaminidase (beta-NAG) is demonstrated in sera from healthy persons and patients with acute and chronic liver diseases by electrofocusing in polyacrylamide gel. Elevation of isoenzyme P is observed only together with elevation of Glutamate-Pyruvate-Transaminase (GPT) in patients with acute and chronic liver diseases of different etiology and activity. The correlation between isoenzyme P and GPT is significant. On the contrary whole beta-NAG is elevated due the activity of the mesenchymal reaction in the injured liver tissue, not related to enzymes of the cytosol like GPT.

Acetylglucosaminidase↗
PubMed1978

Isoenzymes of N-acetyl-beta-glucosaminidase from human liver and serum: separation by electrofocusing in thin layers of polyacrylamide gel.

Isoenzymes of N-acetyl-beta-glucosaminidase can be separated by electrofocusing in thin layers of polyacrylamide gel. Patterns of isoenzymes from human liver tissue and serum show changes in liver diseases. Preliminary results indicate, that N-acetyl-beta-glucosaminidase isoenzyme A is increased in liver tissue from patients with chronic liver diseases in relation to the activity of mesenchyme reaction. The described method will be discussed in comparison with known separation techniques.

Acetylglucosaminidase↗
PubMed1978

[Consideration of the mesenchyme-reaction in patients with cronical liver-diseases (author's transl)].

The lysosomal glycosidase N-Acetyl-beta-glucosaminidase (beta-NAG) is involved in the breakdown of connective tissue. The activity of this enzyme was determined in sera and biopsies of liver tissue from patients with chronic diseases of the liver of different aetiology. Elevation of enzyme activity was found to be related to an increase of the mesenchymeraction in the injured liver tissue. No correlation was found between activity of beta-NAG and of GPT, gammaGT and CHE.

Acetylglucosaminidase↗
PubMed1977

[Lysosomes and lysosomal enzymes in the wall of imbalanced arteries of rats (author's transl)].

The electron microscope demonstration of reaction products of the acid phosphatase within matrix vesicles (=extracellular membrane-bound corpuscles) of the wall of hemodynamic or metabolic imbalanced arteries stimulated experiments about the activities of lysosomal enzymes in the wall of wrongly loaded arteries. In our case arteries without any hemodynamic function (="null function") were investigated 4, 5, 7, 13 and 14 days after applying a ligature. In relation to the nonligated testicular arteries of the other side the wall of theligated vessels show an increase of lysosomal glycosidases (beta-N-Acetyl-glucosaminidase and beta-galactosidase) about 40-70%. The significance of the extracellular lysosomes for transformations of the vessel wall under physiological and pathological conditions is discussed.

Acetylglucosaminidase↗
PubMed1977

[Serum lysozyme concentrations in patients with morbus Crohn before and after bowel resection (author's transl)].

Serum lysozyme (muramidase) concentrations were determined in five patients with Morbus Crohn before and after resection of inflammated bowel areas. The serum lysozyme activity which was elevated before surgical treatment in all patients fell to normal values after bowel resection within a few hours. Our findings suggest, that the elevated serum lysozyme reflects an increased lysozyme production in the inflammated bowel tissue.

Colitis, Ulcerative↗
PubMed1976

[Enzyme activities of beta-hexosaminidase in urine from patients with renal diseases (author's transl)].

Enzymatic activities of beta-hexosaminidase and beta-galactosidase were determined in 24 h urine specimens from patients with renal diseases, from individuals with essential hypertension and patients with other diseases. Patients suffering from various renal diseases had significantly higher activities of the beta-hexosaminidase than individuals with essential hypertension or other diseases. However for beta-galactosidase only the mean value of the enzymatic activities was elevated. The possible mechanisms causing an increase of these enzymes in 24 h urine samples are discussed.

Galactosidases↗