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G Poy

Publications and source records attributed to G Poy.

7 recordsLinked to original sources
PubMed1978

Triggering of cultured neoplastic mast cells by antibodies to the receptor for IgE.

Cell surface receptors for IgE were isolated from detergent lysates of iodinated, IgE-saturated, rat basophilic leukemia cells by precipitation with anti-IgE antibodies followed by chromatography at acid pH. The isolated material showed a single 125I-band (m.w. approximately 58,000) on gel electrophoresis in sodium dodecyl sulfate and was used to immunize a rabbit. The resulting anti-serum was reacted with lysates of surface iodinated mouse or rat tumor mast cells. Analysis of the precipitates on (10%) gel electrophoresis revealed one major peak comprising greater than 80% of the detectable counts and having an estimated m.w. of approximately 58,000. The antiserum reacted with detergent-solubilized and cell-bound receptors in the presence or absence of excess IgE; it also inhibited the binding of 125I-IgE. Cultured mouse mastocytoma cells never exposed to IgE released 3H-serotonin when incubated with F(ab')2, but not Fab' fragments of the antiserum, which had been rigorously freed of IgE and anti-IgE. The release was inhibited in the presence of excess IgE, was Ca++ dependent, and equaled 80% of the maximum obtained with IgE and anti-IgE. We conclude that aggregation of the receptors for IgE provides the critical signals for cell activation.

Animals↗
PubMedDate not supplied

[Bipedal lymphography in mediastino-pulmonary sarcoidosis. Report concerning 9 cases].

The relevant literature is briefly reviewed and lymphographic data obtained from 9 patients admitted to the S. L. Gonzaga Hospital are examined. In all cases, the diagnosis of sarcoidosis had been given prior to lymphography and confirmed histologically. This small series helps to show the extent of lymph node lesions in sarcoidosis and the more commonly observed adenographic features.

Adolescent↗