Biomedical subjects
Publications and source records attributed to G R Knowles.
A previous report in this Journal of a computer method for assessing the size of a protein from its amino acid composition, and its application to virus protein data, contained an error in the published arithmetical formula. Persons using the incorrect formula would not have obtained incorrect estimates of possible protein sizes, but would have obtained a more equivocal set of possible sizes.
A new computerised technique has been devised for measuring the distribution of chiasmata along diplotene bivalents. The method involves the introduction into the field of view of the microscope, of a fine light spot which can be accurately manipulated along the chromosomes of each bivalent. The data recorded include (a) the positions of the chiasmata along the bivalent in terms of their relative distances from the centromere and (b) the individual bivalent and cellular chiasma frequencies. -- The method has been applied to the analysis of chiasma distribution patterns in the two known species of the genus Caledia, C. species nova 1 and C. captiva and in two chromosomal races of the latter. Statistical tests indicate that within bivalents at least 40% of the comparative distribution patterns of chiasmata between races and species are significantly different. Similar comparisons between populations within races reveal only 18% significant differences. -- The observed distribution patterns of chiasmata in this genus suggest that chiasma formation is sequential from centromere to telomere. -- The variation in the frequency and distribution of chiasmata between races and species suggests that the interference distances between successive chiasmata are, at least partially, independent of chiasma frequency and position. -- The interracial and interspecific differences in chromosome structure are correlated with changes in chiasma pattern.