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Biomedical subjects

G R Tudhope

Publications and source records attributed to G R Tudhope.

At least 19 recordsLinked to original sources
PubMed1987

Superoxide dismutase, glutathione peroxidase and catalase in the red cells of patients with malignant lymphoma.

Erythrocyte superoxide dismutase, glutathione peroxidase and catalase activities have been measured in patients with untreated malignant lymphomas. Marked deficiencies of superoxide dismutase (P less than 0.001) and glutathione peroxidase (P less than 0.001) were found, whereas catalase levels were normal. There was no apparent difference in enzyme activities between the different histological types of lymphoma.

Catalase↗
PubMed1979

Thrombocytosis, thrombocythaemia and iron deficiency in patients with polycythaemia vera.

6 patients with polycythaemia vera who also developed concomitant iron deficiency are reported. When oral iron therapy was given, there was a marked reduction in the previously elevated platelet counts in 4 patients; during 2-9 years of observation of these patients there was a significant inverse relationship between haemoglobin concentration and platelet count. In the other 2 patients the elevated platelet counts did not fall when iron therapy was given. The significance of these different patterns of response in relation to the causal mechanism of elevated platelet counts in patients with polycythaemia vera is discussed.

Aged↗
PubMed1979

Spectrophotometric method applicable to in vitro studies of Heinz body formation in erythrocytes.

A simple quantitative procedure is described for the estimation of Heinz body formation in erythrocytes, based on the spectrophotometric measurement of the amount of crystal violet absorbed by precipitated Heinz bodies. Good correlation is shown between this method and the counting of Heinz body-containing erythrocytes in a microscopical preparation. This technique is particularly applicable to in vitro studies of the comparative effects produced in the erythrocyte by oxidant agents.

Erythrocyte Count↗
PubMed1978

Studies with primaquine in vitro: superoxide radical formation and oxidation of haemoglobin.

1. The production of superoxide radicals from primaquine diphosphate in aqueous solution has been demonstrated, using as indicator the reduction of cytochrome C with inhibition of the reaction by superoxide dismutase. 2. Primaquine-mediated oxidation of haemoglobin to methaemoglobin was reduced by the addition of catalase and increased by superoxide dismutase. Mannitol, a hydroxyl radical scavenger, abolished the increase in methaemoglobin observed in the presence of superoxide dismutase. EDTA reduced the oxidation of haemoglobin with and without superoxide dismutase. 3. Although the oxidation of haemoglobin in the presence of primaquine includes the effects of hydrogen peroxide, superoxide and hydroxyl radicals and metal ions, the results indicate that hydrogen peroxide, rather than the superoxide radical, is the main oxidizing species. The increase in haemoglobin oxidation occurring with superoxide dismutase may result from the augmented rate of hydrogen peroxide formation from superoxide radicals.

Catalase↗
PubMed1975

Lipid peroxidation in human erythrocytes in tocopherol deficiency.

Lipid peroxidation following exposure to H2O2 vapour was abnormally great in the erythrocytes from certain patients with low plasma tocopherol concentrations. Following treatment of such patients with tocopherol, erythrocyte lipid peroxidation was markedly reduced. The in vitro addition of tocopherol reduced lipid peroxidation in erythrocytes from both normal subjects and tocopherol-deficient patients.

Aged↗