PubMed HealthSearch

Biomedical subjects

G R Wilson

Publications and source records attributed to G R Wilson.

At least 19 recordsLinked to original sources

Water ingestion does not improve 1-h cycling performance in moderate ambient temperatures.

Eight endurance-trained cyclists rode as far as possible in 1 h on a stationary cycle simulator in a moderate environment (20 degrees C, 60% relative humidity, 3 m.s-1 wind speed) while randomly receiving either no fluid (NF) or attempting to replace their approximate 1.71 sweat loss measured in a previous 1-h familiarisation performance ride at approximately 85% of peak oxygen uptake with artificially sweetened, coloured water (F). During F, the cyclists drank mean 1.49 (SEM 0.14) 1 of which mean 0.27 (SEM 0.08) 1 remained in the stomach at the end of exercise and mean 0.20 (SEM 0.05) 1 was urinated after the trial. Thus, only mean 1.02 (SEM 0.12) 1 of the ingested fluid was available to replace sweat losses during the 1-h performance ride. That fluid decreased the mean average heart rate from 166 (SEM 3) to 157 (SEM 5) beats.min-1 (P < 0.0001) and reduced the final mean serum [Na+] and osmolalities from 143 (SEM 0.6) to 139 (SEM 0.6) matom.l-1 (P < 0.005) and from 294 (SEM 1.7) to 290 (SEM 1.9) mosmol.l-1 (P = 0.05), respectively. Fluid ingestion did not significantly attenuate rises in plasma anti-diuretic hormone and angiotensin concentrations, or decrease the approximate-15% falls in estimated plasma volume in the F and NF trials. Nor did fluid ingestion significantly affect the approximate 1.71.h-1 sweat rates, the rises in rectal temperature (from 36.6 degrees to 38.3 degrees C) or the ratings of perceived exertion in the two trials.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult

Postburn breast reconstruction: tissue expansion prior to contracture release.

Tissue expansion of the scarred chest following burns results in a poor breast mound shape with little projection or inframammary fold, since the expander, like normal developing breast tissue, is kept flat by the scarred skin envelope. We present a case that demonstrates that adequate projection of the breast and formation of an inframammary fold can be achieved by expansion if extensive release and skin grafting of contractures over the breast mound are performed after expansion. Maintained expansion will act as a stent reducing secondary contracture of the grafted-areas.

Adult

Divergent selection for postweaning feed conversion in Angus beef cattle: I. Mean comparisons.

Each year from 1979 through 1983, 35 Angus bull calves were selected from a herd at the Eastern Ohio Resource Development Center to be individually fed in a 140-d postweaning performance test. From these 35 individually fed bulls, the three highest and three lowest for feed conversion (feed:gain) were selected and randomly mated to approximately 20 cows each. A different set of high vs low feed conversion sires was used each year. Four replicates (403 progeny) from high vs low sires were evaluated by sire groups for subsequent postweaning and carcass performance. Progeny were slaughtered when estimated by ultrasonic measurement to have 8.9 mm or more of subcutaneous fat at the conclusion of a 140-d postweaning performance test. Progeny with less than 8.9 mm of subcutaneous fat were fed for additional 28-d periods until they reached the required minimum. No differences were found between high and low feed conversion progeny for 140-d feed intake (P less than .30) although high feed conversion progeny gained .09 kg/d more weight (P less than .01) during the 140-d postweaning test. Differences tended to exist between high and low feed conversion progeny for unadjusted (P less than .15) and maintenance-adjusted (P less than .15) feed:gain ratios. Progeny of the high feed conversion group had greater subcutaneous fat (P less than .05) at the end of the 140-d postweaning test and when slaughtered (P less than .05), indicating a genetic difference for composition of BW gain between high- and low-sired progeny. However, no significant differences existed for any other carcass traits evaluated. Bulls had more desirable unadjusted (P less than .001) and maintenance-adjusted (P less than .001) feed:gain ratios than heifers with increased 140-d ADG (P less than .001) and pen feed intakes (P less than .001).

Animals

Divergent selection for postweaning feed conversion in Angus beef cattle: II. Genetic and phenotypic correlations and realized heritability estimate.

A single generation divergent selection study, replicated four times (1983, 1984, 1985, and 1986), was conducted to assess genetic differences between progeny of high and low feed conversion sires in Angus beef cattle and to determine correlated response for weight gain (ADG140), feed intake (AVFD140), and BW (OFFTSTWT) in a time- (140-d) and fat-constant (8.9 mm) period. Realized heritability estimates for unadjusted (feed/gain; FEFF140; .26) and adjusted feed conversion (adjusted as recommended by the BIF, 1986; ADJFDEFF; .46) were obtained. The difference in heritability estimates reflects variation accounted for by adjustment for BW differences, and thus maintenance requirements, of individual progeny. Phenotypic and "pseudo" realized genetic correlations of FEFF140 with ADG140, AVFD 140, and OFFTSTWT were -.33 and -.66, .49 and -.26, and .15 and -.41, respectively. Phenotypic and "pseudo" realized genetic correlations of ADJFDEFF with ADG140, AVFD140, OFFTSTWT, and FEFF140 were -.54 and -.59, .30 and -.23, .27 and -.36, and .97 and .49, respectively. Subcutaneous fat (as estimated by ultrasonic measurement; BF140) had phenotypic and "pseudo" realized genetic correlations with FEFF140 of -.33 and .66, respectively, and with ADJFDEFF of -.44 and -.58, respectively.

Animals

Activation and germination of Bacillus thuringiensis spores in Manduca sexta larval gut fluid.

Incubation of Bacillus thuringiensis HD-1 spores in the larval gut fluid of Manduca sexta (tobacco hornworm) resulted in increased viable counts, conversion to phase-dark spores, and a loss of absorbance in spore suspensions, indicative of spore germination. Heat-activated and untreated spores incubated in water did not exhibit these changes. Only when spores were heat activated and incubated in germinants L-alanine and adenosine did changes in the spores approximate those observed in gut fluid. These data suggest that M. sexta larval gut fluid induces the activation and germination of B. thuringiensis spores.

Animals

Plasmid-associated sensitivity of Bacillus thuringiensis to UV light.

Spores and vegetative cells of Bacillus thuringiensis were more sensitive to UV light than were spores or cells of plasmid-cured B. thuringiensis strains or of the closely related Bacillus cereus. Introduction of B. thuringiensis plasmids into B. cereus by cell mating increased the UV sensitivity of the cells and spores. Protoxins encoded by one or more B. thuringiensis plasmids were not involved in spore sensitivity, since a B. thuringiensis strain conditional for protoxin accumulation was equally sensitive at the permissive and nonpermissive temperatures. In addition, introduction of either a cloned protoxin gene, the cloning vector, or another plasmid not containing a protoxin gene into a plasmid-cured strain of B. thuringiensis all increased the UV sensitivity of the spores. Although the variety of small, acid-soluble proteins was the same in the spores of all strains examined, the quantity of dipicolinic acid was about twice as high in the plasmid-containing strains, and this may account for the differences in UV sensitivity of the spores. The cells of some strains harboring only B. thuringiensis plasmids were much more sensitive than cells of any of the other strains, and the differences were much greater than observed with spores.

Bacillus cereus

Childhood illness as an aetiological factor in burns.

A prospective study of 208 children has shown that a significant number of them have an illness before suffering a thermal injury. The authors suggest that an unwell child is more likely to suffer a burn, and that first aid programmes should stress to parents to be even more careful when their child is unwell.

Accidents, Home

Predicting successful brachial plexus block using changes in skin electrical resistance.

Skin electrical resistance is determined by the degree of sweating of the skin which is, in turn, related to sympathetic nervous system activity in the area concerned. It is increased when the nerves supplying the area are damaged or blocked by local anaesthetic agents. We have assessed the temporal and spatial relationship between the onset of sympathetic and sensory loss in the hand following brachial plexus block in 44 patients. Skin electrical resistance, measured using a simple ohm meter, has been shown to increase within 2 min of brachial plexus blockade with 1% lignocaine and adrenaline 1:200,000. This increase is an early and reliable indicator of subsequent, and occasionally delayed, sensory loss.

Aged

Loss of split thickness skin grafts due to non-group A beta-haemolytic streptococci.

Over a 17-month period 77 patients requiring a split skin graft for a burn injury have suffered loss of previously well taken graft due to the growth of a beta-haemolytic streptococcus. Of these only 42 were streptococci of Lancefield group A (Streptococcus pyogenes); 16 were group B, 3 group C and 16 group G. Some strains of groups B, C and G produce cytopathic and spreading factors capable of destroying the new skin graft and regenerating epithelium. We suggest that the non-group A streptococci may be more pathogenic than previously recognised in this particular respect.

Burns

A new burn area assessment chart.

A series of body charts have been designed that are more representative of changing body proportion with increasing age. Being easier to use, these charts have led to a better estimate by the casualty doctor of the body surface area that has been burned.

Adolescent

The muscle twitch monitor: a rapid unequivocal method of monitoring free muscle transfers.

A free latissimus dorsi muscle transfer, which required anastomotic revision on two occasions within twenty-four hours of operation, illustrates how the change in response of electrically induced muscle contraction to ischaemia can be used as a rapid and sensitive method of monitoring free muscle transfers. It is a response that has the great merit of being easily recognised by untrained observers because it relies on an absolute rather than a relative change in the measured modality. Furthermore it is not influenced by the clinical condition of the patient or by changes in the flap's environment. Its potential is discussed.

Adult

Evaluation of a new antiseptic dressing in minor burns.

A prospective trial was conducted to compare a new povidone iodine impregnated dressing (Inadine) with a standard petroleum jelly gauze dressing for small superficial burns treated on an outpatient basis. The results show no difference between comfort and ease of removal of dressings, in the number of positive bacteriological cultures or the number of days to healing. Inadine is more than twice as expensive as petroleum jelly gauze.

Adolescent

Full thickness burns from ready-mixed cement.

The authors present 16 cases of cement burns admitted to the Nottingham burns unit over an 11-month period. Thirteen required skin grafting and were off work for a considerable period of time. The manufacturers do print warnings on their quotations and their delivery dockets, but the serious nature of some cement burns is not stressed. Some suggestions are made in an effort to prevent these serious injuries.

Adolescent

A simple method of measuring the electrical resistance of the skin.

A simple device has been developed to measure skin resistance. Normal values have been established and the effect of changing measurement parameters has been investigated. Peripheral nerve blocks have also been applied and their effect upon the skin resistance reported.

Adolescent