The mentoring relationship: what makes it work?
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Education, Nursing↗
Biomedical subjects
G Reichert
Publications and source records attributed to G Reichert.
Increased amount of a mitochondria-associated 21 x 10(3) dalton protein in human gastrointestinal tumors.
Differences in the structure and number of mitochondria in tumor cells were found. Using isoelectrofocusing two-dimensional polyacrylamide gel electrophoresis which allows detection of alterations in the protein pattern of tumor mitochondria, we studied both quantitative and qualitative changes in the mitochondrial protein pattern of human gastrointestinal tumors and corresponding normal matrix tissues. One low molecular protein spot was found to be quantitatively changed in the tumors. The approximate molecular weight was 21 x 10(3) daltons and the pI value 5.7.
Adult↗
Characterization of native 40 S particles from Krebs II mouse ascites tumor cells: resolution, nomenclature and molecular weights of the nonribosomal proteins.
Native 40 S particles from Krebs II mouse ascites tumor cells were isolated on a large scale. A nonribosomal protein moiety of about 30 proteins could be removed from the ribosomal particles by treatment with 250 mM KCl. These proteins were analysed by two-dimensional polyacrylamide gel electrophoresis and turned out to be mostly acidic in nature. The molecular weights of about 17 proteins were determined by three-dimensional gel electrophoresis. Radioactively labelled nonribosomal protein spots were excised from two-dimensional gel electrophoresis. Radioactively labelled nonribosomal protein spots were excised from two-dimensional gels and transferred directly or after electrodialysis onto the third dimension gel. The proteins fell into a molecular weight range from about 20,000 to 300,000.
Animals↗
Phosphorylation in vivo of non-ribosomal proteins from native 40 S ribosomal particles of Krebs II mouse ascites-tumour cells.
Four non-ribosomal proteins from native 40 S ribosomal subunits with mol.wts. of 110 000, 84 000, 68 000 and 26 000 were phosphorylated in vivo when ascites cells were incubated in the presence of [32P]Pi. The 110 000-, 84 000- and 26 000-dalton proteins are identical with phosphorylated products from native 40 S subunits after phosphorylation in vitro by a cyclic nucleotide-independent protein kinase. Phosphoserine was the major phosphorylated amino acid of the proteins phosphorylated in vivo and in vitro.
Amino Acids↗
Characterization of native 40S particles from krebs II mouse ascites tumor cells. I. Phosphorylation in vitro of non ribosomal proteins by a cAMP independent protein kinase.
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Adenosine Triphosphate↗
Comparison of alterations in the histamine-diamine oxidase system during acute intestinal ischaemia in pigs, dogs and rabbits; evidence for a uniform pathophysiological mechanism?
Among various vasoactive substances histamine was also suggested to induce circulatory arrest following superior mesenteric artery occlusion. Thus the involvement of the histamine-diamine oxidase system was studied in intestinal ischaemia using three animal species. In pigs, dogs and rabbits aminoguanidine, the specific inhibitor of diamine oxidase, shortened the survival time after mesenteric infarction. Under these conditions the diamine oxidase activity in the intestinal wall was reduced in animals treated by saline whereas the histamine content was not altered significantly. Plasma histamine levels were increased considerably in the portal vein of pigs during the revascularization period if the animal were pretreated by aminoguanidine. Similar findings were obtained in dogs. It was concluded that in all three species investigated the diamine oxidase protects the organism against the deleterious effects of at least one of its vasoactive substrates-histamine.
Amine Oxidase (Copper-Containing)↗
Diamine oxidase activity in gastric and duodenal mucosa of man and other mammals with special reference to the pyloric junction.
In the gastric mucosa of human subjects and of various mammals methylation was accepted as the main pathway of histamine catabolism. However, augmentation of gastric acid secretion by aminoguanidine, the strong inhibitor of diamine oxidase, indicated an influence of diamine oxidase activity on this secretory process. Therefore a careful reinvestigation of the occurrence of diamine oxidase activity was started from the distal duodenum in the direction of the cardia. In all species studied, diamine oxidase activity decreased from distal duodenum towards the pylorus. In dogs, landrace pigs and in human subjects the diamine oxidase activity clearly exceeded the pyloric borderline gradually becoming zero in corpus or fundus. In rabbits, however, and especially in mini-pigs no diamine oxidase activity was found beyond the pylorus. Among individuals gastric diamine oxidase activity showed a variable prevalence and could not be found regularly in all the subjects. In one patient with prepyloric ulcer a strong influence of pathophysiological processes on gastric diamine oxidase activity could be suspected. Thus, in every alteration of the gastric mucosa under experimental or clinical conditions also an alteration of gastric diamine oxidase activity should be taken into account.
Aged↗
Diamine oxidase activity and histamine release in dogs following acute mesenteric artery occlusion.
Following superior mesenteric artery occlusion and revascularization in dogs all animals died in a circulatory collapse state. However, pretreatment by aminoguanidine, the strong and specific inhibitor of diamine oxidase, accelerated the circulatory break-down significantly and increased the venous plasma histamine concentrattions up to levels which also in normal dogs are effective in the circulatory system. Furthermore, the haematocrit increased significantly more in the aminoguanidine-treated animals than in the dogs treated by saline. No changes in plasma diamine oxidase activity were observed in saline-treated animals during intestinal ischemia and following revascularization. In aminoguanidine-treated animals no enzymic activity could be measured. The results were interpreted by a protective role of intestinal diamine oxidase in intestinal ischemia. Enhancement of the enzymic activity in patients, for instance by heparin, may be helpful in mesenteric infarction disease.
Amine Oxidase (Copper-Containing)↗