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Biomedical subjects

G Reiner

Publications and source records attributed to G Reiner.

85 records · Page 5Linked to original sources
PubMed1984

Different suitability of 3 filarial antigens (Litomosoides carinii, Dipetalonema viteae, Dirofilaria immitis) to act as allergens in the Passive Cutaneous Anaphylaxis Test and to serve as antigens in an ELISA in the course of experimental filarial infections (L. carinii, D. viteae, Brugia malayi, B. pahangi) of Mastomys natalensis.

Crude extracts of adult worms of 3 different filariae (Litomosoides carinii, Dipetalonema viteae, Dirofilaria immitis) were evaluated for their suitability to serve as allergens in the Passive Cutaneous Anaphylaxis Test (PCA) and as antigens in an ELISA which detected mainly IgG antibodies. Studies were done in the course of 4 different filarial infections (L. carinii, D. viteae, Brugia malayi, B. pahangi) of Mastomys natalensis, using sera from different times after infection up to 350 days p.i.--In the PCA L. carinii and D. viteae antigens, apart from L. carinii infection caused reactions to a similar degree. In the L. carinii infection the homologous antigen was more effective. The D. immitis antigen was clearly less effective: high titres were found only during the early prepatency of D. viteae and Brugia infections and during the early patency of L. carinii and D. viteae infections. In all other cases, if at all, it led to weak reactions only.--In the ELISA different time courses were obtained as well. In Brugia infections values obtained with D. viteae and D. immitis antigens were significantly correlated but were not related to those obtained by the L. carinii antigen. However, the L. carinii antigen detected high levels of antibodies especially during prepatency of B. malayi, B. pahangi and D. viteae infections. No relations were found between the antigens for the D. viteae infection. In the case of the L. carinii infection the values of all 3 antigens were significantly correlated.

Allergens↗
PubMed1983

Time courses of antibody levels in Mastomys natalensis after infections with Litomosoides carinii, Dipetalonema viteae, Brugia malayi or B. pahangi, Determined by ELISA.

Using a Litomosoides carinii adult antigen, time courses of antibody levels were followed by an ELISA in L. carinii, Dipetalonema viteae, Brugia malayi and B. pahangi infected Mastomys natalensis. Using various groups of infected animals, periods up to 400 days after infection were covered. In L. carinii infected Mastomys, antibodies were first detected 11 days p.i. and levels increased rapidly until day 40. Temporarily reduced levels about the beginning of patency were followed by increasing values until about 100 days p.i. Then the antibody content of the sera remained more or less constant until about 250 days p.i. although maximum levels were found at day 170. Thereafter, the antibody concentration in the sera declined slowly but high levels were still observed 390 days p.i. The antibody content was usually higher in animals with high microfilariae densities than in those with low microfilariae counts but relations could not be proven statistically. In D. viteae infected Mastomys, maximum antibody values were reached within the beginning of patency. Levels were not altered markedly until about 110 days p.i. Thereafter they decreased slightly but then remained constant until the end of the investigation period 350 days p.i. B. malayi infected animals showed a rapid increase of the antibody content in the sera; a maximum was reached by 20 days after the infection. Thereafter, somewhat constant levels were found for 4--5 months. After 300 days p.i. the antibody levels declined progressively, accompanied with increasing parasitaemia densities; after 380 days the levels reached about 2/3 of the maximum. However, despite this, no relation was found between the levels of parasitaemia and antibody in individual animals. In B. pahangi infections the main prepatent antibody increase occurred during week 5 p.i., when maximum values were observed. The beginning of patency and the early patency were accompanied with slightly declining antibody levels. From 150 days p.i. until the end of the investigation 400 days p.i., the antibody content of the sera was fairly constant.

Animals↗
PubMed1983

Clinical and experimental aspects of hormone receptors in breast carcinoma patients.

Estrogen (ER) and progesterone (PgR) receptor contents were determined in 214 patients with primary mammary carcinoma. Premenopausal and postmenopausal females (43 and 48%, respectively) were found to be ER and PgR positive. ER levels between 100 and 500 fmol/mg protein were observed in 37% postmenopausal and 14% premenopausal patients, respectively. ER distribution between lymph node-positive and -negative patients did not differ significantly. Subjects without detectable ER had recurrences earlier than did subjects with ER. On the other hand, no differences in recurrence rates were noted when PgR-positive and -negative patients were compared. In addition, the determination of ER did not permit selection of patients for adjuvant cytotoxic chemotherapy. Furthermore, the action of tamoxifen was investigated in the rat pituitary as a model system. Tamoxifen was found to act as antiestrogen at the level of transcription by inhibiting the accumulation of estrogen-stimulated preprolactin mRNA. Combined data suggest that patients with positive lymph nodes and positive receptors may benefit from combined endocrine plus cytotoxic adjuvant chemotherapy.

Animals↗
PubMed1981

[Steroid receptor levels in human breast carcinomas in relation to serum hormone levels in postmenopausal patients (author's transl)].

Serum levels of LH, FSH, HPRL, oestradiol, progesterone and testosterone were determined by radioimmunoassay in 45 postmenopausal patients in the morning before they were operated on for primary breast cancer. Cytosol oestradiol - (E2R) and progesterone receptor (PgR) levels were estimated by Scatchard plot analysis of saturation assays using the charcoal method. When serum hormone levels and receptor contents were correlated according to the distribution free test of Spearman, all correlations were found to be insignificant, with the exception of E2R/PgR (p less than 0.001).

Aged↗
PubMed1981

[Tumor histology and steroid receptors in breast carcinoma].

In Specimens of 115 patients with breast cancer 4 tumorparameters (tumorsize, tumorboder, nucleargrade, lymphocytic stromal reaction) 3 features of regional lymphnodes (sinushistiocytosis, T-cellreaction, lymphnode metastases) and estrogen and progesteron receptors were determined. A strong sinushistiocytosis and T-cellreaction could be verified mainly in metastases in free lymphnodes. The steroid receptor content does not correlate with histological parameters.

Breast Neoplasms↗
PubMed1980

[Oestrogen receptors and prognosis in breast cancer].

The determination of oestrogen receptors seems to be of prognostic value in the radically-operated breast cancer patient. Patients with negative receptor values exhibit early recurrence in a significantly higher percentage of cases than those with positive values. The prognostic implications of oestrogen receptor determination seem to be even higher than an assessment of axillary lymph node involvement. In metastatic breast carcinoma a correlation exists between response to hormonal therapy and receptor assay. The clinical implications for therapeutic management are discussed.

Adult↗
PubMed1980

[Prognostic value of estrogen receptors in breast cancer (author's transl)].

One hundred and ninety-one patients with operable breast cancer were followed for up to 34 months after operation and the early recurrence of disease was noted in relation to the presence or absence of estrogen receptor. Recurrence rates were significantly higher in patients whose tumors did not contain receptors than in those whose tumors did. The response to cytotoxic chemotherapy in patients with advanced breast carcinoma seems to be independent of the presence of E2R in cancer tissue.

Breast Neoplasms↗
PubMed1979

[Prognostic factors in the drug therapy of metastasizing breast cancer].

175 patients with metastatic breast cancer, treated with chemotherapy, were analyzed retrospectively to identify the characteristics of prognostic importance in predicting response to chemotherapy and survival from onset of the chemotherapy. The most significant factors were the sites of metastatic disease and an estimate of the total extent of disease.

Antineoplastic Agents↗
PubMed1979

[Various amounts of estrogen receptors in breast neoplasms].

An agar gel electrophoresis was performed to demonstrate various amounts of estrogen receptors of three different topics in 38 mammary carcinomas. A good correlation between estrogen receptors in primary mammary carcinomas and axillary lymph node metastases could be found.

Breast Neoplasms↗
PubMed1979

[Topical differences in estrogen receptor content of primary breast cancer].

In 50 primary cancers of the mammary gland, tissue samples from three different parts of each tumor were analyzed for estrogen content by agar gel electrophoresis. In 12 tumors receptor-rich and receptor-poor zones were detected within the tumor for the 4-s fraction, and in 11 tumors for the 8-s fraction. Confidence limits (99%) indicate that these findings might range from 10.4% to 42.5% for the 4-s fraction, and 10.2%-44.1% for the 8-s fraction.

Breast Neoplasms↗
PubMed1979

[Childhood injuries treated at a university clinic for traumatology (author's transl)].

The accidents to all children up to the age of 14 years admitted to the I. Universitätsklinik für Unfallchirurgie in Wien in 1976 (2977 children) were analysed as to age-distribution, cause of accident, type and location of injury. There was an increase in accidents to older children. 13% of the injuries were due to traffic accidents; 60% occurred in the course of play; 27% on the field of sport. More than 60% were only minor injuries such as abrasions, lacerations, crushed fingers and toes etc., 30% bone fractures and ligamental injuries; 2,5% brain traumas. Most of the bone fractures were localized iment and no lethal injuries.

Accidents↗
PubMedDate not supplied

Diagnosis and treatment of bleeding colonic diverticula.

BACKGROUND/AIMS: Colonic diverticula are the most frequent cause of major lower intestinal bleeding and pose a diagnostic and therapeutic challenge to the attending physician. Emergency surgical resection is associated with a high mortality and morbidity and patients who will stop bleeding spontaneously cannot be distinguished from those who will continue to bleed. Our aim was to evaluate the efficacy of barium enema as a sole less invasive treatment option for severe diverticular bleeding. METHODOLOGY: We evaluated 102 patients admitted with colonic diverticular bleeding, from 1993 to 1997, who needed transfusion of 2 or more units of blood. We compared the clinical efficacy of surgical resection, conservative treatment, and therapeutic barium enema with regard to the cessation of bleeding, morbidity, mortality, and rebleeding rate. The therapeutic strategies used after further episodes of bleeding were also registered. RESULTS: Transfusion requirements were highest in patients who underwent surgical treatment, while the least amount of blood was required by the barium enema group (6.9 +/- 3.1 vs. 3.6 +/- 1.5 units of blood). However, the quantity of transfused blood did not correlate with the initial hemoglobin level, which was highest in the conservative treatment group and lowest in the operative group (9.0 +/- 1.2 vs. 8.1 +/- 1.3 g/dL). These data support the fact that the most severe bleeding would necessitate surgical resection and that therapeutic barium enema may be considered more effective than conservative treatments. With regard to the outcome of treatment, conservative treatment led to a rebleeding rate of 43.3%, which differed significantly from a 15.9% rebleeding rate after therapeutic barium enema (P = 0.009). No rebleeding was registered in surgically treated patients. Sixty percent of patients in whom therapeutic barium enema failed were treated by colonic resection without mortality, while 77% of patients who had rebleeding after conservative treatment were successfully treated with barium enema. Overall, barium enema was the most frequently applied second-line treatment (56.5%). The mortality after surgery was significantly higher than that after other treatment modalities (33% vs. 1%; P = 0.0001). CONCLUSIONS: If diverticular bleeding is clinically suspected as the cause of major lower intestinal hemorrhage, barium enema is a more promising alternative than conservative treatment because of diagnostic and therapeutic importance in the long-term. In the event of urgent secondary surgery following the failure of barium enema to stop bleeding, we recommend a sigmoidoscopy and, optionally, an angiography before surgery in order to first localize the bleeding. We conclude that therapeutic barium enema is the treatment of choice for the first bleeding episode, while surgical resection should be performed if rebleeding occurs.

Aged↗