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G S Bilaspuri

Publications and source records attributed to G S Bilaspuri.

18 recordsLinked to original sources
PubMed2004

A quantitative study of seminiferous tubular cells in the developing Murrah buffalo testis.

We report here a systematic quantitative study of the seminiferous tubular cells of Murrah buffaloes. The most advanced germ cell types in the different age groups (months) were A(0) spermatogonia (SG) (1 and 3), early pachytene (6 and 9), late pachytene (12), secondary spermatocytes (15 and 18), elongating spermatids (21 and 24), elongated spermatids attached to Sertoli cells (30), elongated spermatids detached from Sertoli cells (36) and spermatozoa (42 and 48). Central primitive Sertoli cells (CPSC) and basal primitive Sertoli cells (BPSC) were present in the sex cord of one-month-old calves, while Sertoli cells (SC) were first seen in nine-month-old calves. The number of gonocytes were maximal at six months but they were not seen after this time. Prespermatogonia (PSG) and SG were at a maximum at nine months of age but PSG were not seen after 36 months. The number of SG decreased significantly after nine months up to 36 months of age. Although spermatocytes and spermatids appeared in earlier developmental stages, a rapid increase in their number was recorded after 36 months. The number of SC was maximal in 18-month-old animals. BPSC predominated in the sex cord of animals aged one to six months, SG at 9-12 months of age, primary spermatocytes from 15-30 months and spermatids from 36 to 72 months and in older animals. We concluded that a decrease in the number of SG in buffalo calves after nine months of age might be responsible for a delay in sexual maturity. Moreover, the small number of spermatocytes and spermatids present before 36 months of age may be associated with the low yield of different germ cell divisions and with the cellular degeneration. A rapid increase in the number of spermatocytes and spermatids after 36 months resulted in sexual maturity between 42 and 48 months.

Age Factors↗
PubMed2000

Changes in interstitial cells during development of buffalo testis.

Interstitial cells were identified and counted in the testis of Murrah buffalo calves and bulls at the age of 1, 3, 6, 9, 12, 15, 18, 21, 24, 30, 36, 42, 48 and 72 months and older. Six types of cells were identified in the testicular interstitium of 1-month-old calves. These were mesenchymal cells, fetal type Leydig cells, fibroblasts, myoid cells, pericytes and endothelial cells. Adult Leydig cells were visible in 3-month-old calves, but mesenchymal cells were not seen from 18 months onwards. The percentage of mesenchymal cells reached a maximum in 1 month, fetal type Leydig cells in 3 months, adult Leydig cells in 72 months and beyond, fibroblasts in 36 months, myoid cells in 18 months, pericytes in 21 months and endothelial cells after 15 months. Changing percentages of various interstitial cells revealed that myoid cells may have differentiated into fibroblasts and mesenchymal cells, which then differentiated into adult Leydig cells.

Animals↗
PubMed1997

Cyclophosphamide-induced structural and biochemical changes in testis and epididymidis of rats.

Effect of cyclophosphamide administration (100 mg/kg body weight, ip, for 5 consecutive days) was studied on albino rat testis and epididymidis after 3 and 6 weeks of treatment. Cyclophosphamide decreased testis and cauda epididymidal weights, sperm count, motile and viable spermatozoa and increased percentage of abnormal spermatozoa. The biochemical changes observed in the testis include increase in acid and alkaline phosphatase activities and decrease in proteins and activity of lactate dehydrogenase. The levels of lipids and total cholesterol were not affected. In the epididymidis cyclophosphamide caused decrease in the tubular diameter and increase in its epithelial height. It is concluded that cyclophosphamide brings about structural and biochemical changes in the testis and epididymidis.

Animals↗
PubMed1994

Spermatogenic cells and stages of the seminiferous epithelial cycle in the Indian gerbil field rat, Tatera indica.

Four types of spermatogonia (A0, A, In, B) are distinguished in T. indica. Four generations of A type, a single generation of intermediate (In) and two generations of B type spermatogonia are identified. Seven divisions of spermatogonia have been observed, in Stages 1b, 2b, 3b, 4b, 5, 6 and 7 respectively. Spermatocytes have been observed in prophase, metaphase, anaphase and telophase; in terms of size and morphology, the phases of prophase could be further divided. Pre-leptotene and leptotene appear in Stage 8 and Stage 1 respectively. Round spermatids become asymmetrical in Stage 1b. For the first time in rodents, Stages 1, 2, 3, 4 and 8 have been subdivided. The percentage frequencies of Stages 1-8 are 12.93, 10.69, 17.54, 10.33, 3.76, 11.20, 7.88 and 25.67 respectively. Although the morphology of the spermatogenic cells and associations of these cell types are basically similar to those in albino rats, T. indica markedly differs from all other rodents studied in having two generations of B spermatogonia and subdivisions of Stages 1, 2, 3, 4 and 8; in addition, the relative frequencies of the stages distinctly differ from those recorded in previous studies of rats. Hence, the durations of different cell types in T. indica may differ markedly from those in other rodents.

Animals↗
PubMed1991

Histochemical changes in adenosine triphosphatase activity during folliculogenesis and corpus luteum formation and regression in the rat ovary.

Histoenzymological changes in Adenosine triphosphatase (ATPase) activity were studied during folliculogenesis in immature and mature rat ovary. Its presence in oocytes of small follicles and absence in those of large follicles postulate a correlation between their absorptive mechanism during the development of the oocyte. The presence of ATPase activity in the theca, corpora lutea and interstitial gland tissue may be related to the vascular endothelium which is associated with the transport system across the membrane.

Adenosine Triphosphatases↗
PubMed1986

The seminiferous epithelial cycle and spermat ogenesis in rams (ovis aries ).

The efficiency of spermatogenesis and degenerations of different spermatogenic cells under normal conditions of the environment have been investigated in rams. The meiotic divisions and the position of first-generation spermatids in haematoxylin-eosin stained testicular preparations were used to identify eight stages of the seminiferous epithelial cycle (SEC). The stages of relatively long duration (i.e., 1,2,3,4,8) were sub-divided. The percent-ages of frequency for the 14 stages reported were also studied. Three generations of type A (A(1), A(2), A(3)), one generation of type intermediate (In) and two generations of type B (B(1), B(2)) spermatogonia were recognized. A(2) and B(2) spermatogonia as well as primary and secondary spermatocytes did not degenerate. Contrarily, A(1), A(2), A(3), In and B(1) spermatogonia showed 25, 13.7, 27.3 and 21.2% degenerations respectively. We concluded that compared with the previously used eight-stage classification, subdividing stages with long durations as done in this study facilitates investigating the degenerations of spermatogenic cells. The efficiency of spermatogenesis in rams was 47.58% since one A(3) spermatogonium produces 30.45 spermatids/spermatozoa against the expected number of 64.

Journal Article↗
PubMed1985

Cytochemical studies on the basic nuclear proteins of buffalo, goat and ram spermatozoa.

A cytochemical study of the basic nuclear proteins in buffalo, goat and ram spermatozoa has been made. The various techniques used suggest that these basic proteins are different from somatic proteins. Free, non-DNA-associated and RNA-associated basic proteins are absent. The basic proteins are rich in arginine, poor in lysine and consist of proteins (histones) other than protamines. It is concluded that these sperm histones are stable proteins or basic keratins. They are more concentrated in the distal portion of the head as compared to the proximal one. The results have been compared with those obtained in other animals.

Animals↗
PubMed1984

Histochemical studies on steroid dehydrogenases in the testis of the goat (Capra hircus).

Detailed histochemical localization of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 17 beta-HSD was made in the goat testis using both NAD and NADP coenzymes. The substrates used for 3 beta-HSD were dehydroepiandrosterone (DHA) and pregnenolone whereas 17 beta-HSD was localized with testosterone and oestradiol. In general, the activity of the enzymes varied with the cell type, substrate and coenzyme. In seminiferous tubules, DHA and NAD were the preferred substrate and coenzyme respectively for 3 beta-HSD. In addition, in interstitial tissue, NAD was the preferred coenzyme with DHA whereas no such preference existed with pregnenolone. 17 beta-Hydroxysteroid dehydrogenase showed a similar pattern in the two main compartments of the testis, as testosterone and oestradiol were equally utilized and NAD was the preferred coenzyme in both these compartments. The activities of the enzymes increased during the process of spermiogenesis and were higher in seminiferous tubules than in interstitial tissue, especially in elongated spermatids and spermatozoa.

17-Hydroxysteroid Dehydrogenases↗
PubMed1983

Histochemical distribution of sudanophilic and unsaturated lipids in the testes of buffalo, goat, and ram.

The sudanophilic and unsaturated lipids have been histochemically localized and correlated with seminiferous epithelial cycle in the testes of buffalo (Bubalus bubalis), goat (Capra hircus) and ram (Ovis aries). All sudanophilic lipids are unsaturated. The masked lipids may be present either in small quantity or absent altogether. In the seminiferous tubules, 3 types of lipid bodies (L1, L2, L3) are present. L3 bodies in the buffalo are of relatively small size but are more in number. L2 bodies appear only in the elongated spermatids. All spermatogenic cells also have diffused sudanophilic lipids. Sertoli cells contain only L1 and L3. The lipids in the spermatids and Sertoli cells show cyclic variations reverse to each other. In the interstitial tissue, the amount of lipids is much less in goat and ram as compared to that in buffalo.

Animals↗
PubMed1983

Effects of different fixatives on the development of acrosomal system during spermiogenesis in buffalo, goat and ram.

The effects of Zenker-formol, Carnoy, Orth and Bouin's fixatives on the development of acrosomal system during spermiogenesis in buffalo, goat and ram were compared. Using Zenker-formol, Orth and Bouin's fixatives it was found in the Golgi and cap phases that the acrosome is derived from the inner and outer zones of acrosomal system. With Carnoy's fixative it was seen that the weakly stained granules are surrounded by a vacuolar space. In the acrosome and maturation phases the acrosome and head cap stain uniformly in the tissue fixed in Zenker-formol, Orth and Bouin's fixatives. In Carnoy fixed tissue the PAS-positive material in the spermatids is weakly stained.

Acetates↗
PubMed1982

Distribution of oxidases in the testis of buffalo, goat and ram: an histochemical study.

Peroxidase, monoamine oxidase (MAO) and cytochrome oxidase (CCO) have been histochemically localized in the testis of buffalo, goat and ram. The results in these three species were more or less similar. Peroxidase was localized only in the interstitial tissue and could be used as a marker enzyme for this testicular compartment. MAO and CCO were present in both the interstitial tissue and the seminiferous tubules. The detailed patterns of MAO and CCO distribution were also similar and showed cyclic changes in the spermatids which have not been reported before. The results have been compared and contrasted with those of previous workers and their possible physiological significance discussed.

Animals↗
PubMed1980

Quantitative studies on spermatogenesis in buffalo (Bubalus bubalis).

The qualitative behaviour of spermatogenetic cells and Sertoli cells in the buffalo (Bubalus bubalis) was studied by identifying 8 stages of the seminiferous epithelium cycle based on meiotic division and morphology as well as the position of more mature spermatids. To achieve better results, stages 1, 2, 3, 4 and 8 were sub-divided. Six peaks of spermatogonial mitosis suggested six generations of spermatogonia. Of these, three were due to A (A1, A2, A3), two to B (B1, B2) and one to intermediate spermatogonia. However, another category of spermatogonia (A0), which divided rarely, were also present at all stages of the cycle. A1, A3, ln-, B1-spermatogonia and secondary spermatocytes showed 20, 18.75, 10.2, 15.5 and 10 p. 100 degeneration, respectively, No degeneration was observed in A2 and B2-spermatogonia, primary spermatocytes or spermatids. The degeneration of these various spermatogenetic cells greatly affected the efficiency of spermatogenesis.

Animals↗
PubMed1976

Stages of seminiferous epithelial cycle and relative duration of spermatogenic processes in the buffalo (Bos bubalus).

By using haematoxylin-eosin preparations, the seminiferous epithelial cycle (SEC) in the buffalo has been divided into 8 stages; stages 1, 2, 3, 4 and 8 have been subdivided. The percentage frequency of these stages and substages has been determined by using the serial sections. The approximate relative duration for the various spermatogenic cells and processes has been worked out from the percentage frequency of a substage or a stage, and the number of substages or stages in which the particular cell or the cell phase was present. The total approximate duration of spermatogenesis in the buffalo is 457 units, which has been divided by the duration of one SEC (i.e. 100 units). The buffalo spermatogenesis is constituted by 4.57 cycles of seminiferous epithelium. The results of the present studies have been compared and contrasted with those of previous studies on various mammalian species including farm animals. For its kinetics of spermatogenesis, the buffalo resembles the bull closely.

Animals↗