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G S Wootton

Publications and source records attributed to G S Wootton.

3 recordsLinked to original sources
PubMed1975

The ratio of smooth muscle cell volume to cell surface area measured by electron microscopy with corrections for shrinkage.

The ratio of cell volume to cell surface area (V/A) has been measured by electron microscopy, for smooth muscle cells of the guinea-pig taenia coli. The extent of shrinkage which occurs during processing for electron microscopy has been estimated using Archimedes' principle. The corrected ratio of V/A is 0.8 mum which is lower than previously published estimates for this tissue. The contribution of the surface vesicles to V/A was not included in the present estimate, but the extra surface area contributed by these organelles would lower V/A even more.

Animals↗
PubMed1975

An association between mitochondria and vesicles in smooth muscle.

Two methods are described for measuring the mitochondrion-vesicle association seen by electron-microscopy in thin sections of the guinea-pig taenia coli. Both methods are based on comparisons of the observed distributions with predicted random distributions. It was found in control muscles that mitochondria were consistently nearer to vesicles than corresponding random points. 1 mM ouabain treatment reduced the mitochondrion-vesicle association for mitochondria which were closer to the membrane surface than 130 nm. Quantitative investigation of the freeze-etch structure of the membrane fracture faces is also reported, confirming the observation that membrane particles are more numerous in vesiculated membrane regions of smooth muscle.

Animals↗