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G Sansone

Publications and source records attributed to G Sansone.

At least 19 recordsLinked to original sources
PubMed2006

Isolated single-cycle attosecond pulses.

We generated single-cycle isolated attosecond pulses around approximately 36 electron volts using phase-stabilized 5-femtosecond driving pulses with a modulated polarization state. Using a complete temporal characterization technique, we demonstrated the compression of the generated pulses for as low as 130 attoseconds, corresponding to less than 1.2 optical cycles. Numerical simulations of the generation process show that the carrier-envelope phase of the attosecond pulses is stable. The availability of single-cycle isolated attosecond pulses opens the way to a new regime in ultrafast physics, in which the strong-field electron dynamics in atoms and molecules is driven by the electric field of the attosecond pulses rather than by their intensity profile.

Journal Article↗
PubMed2006

Generation of high-energy self-phase-stabilized pulses by difference-frequency generation followed by optical parametric amplification.

We produce ultrabroadband self-phase-stabilized near-IR pulses by a novel approach where a seed pulse, obtained by difference-frequency generation of a hollow-fiber broadened supercontinuum, is amplified by a two-stage optical parametric amplifier. Energies up to 20 microJ with a pulse spectrum extending from 1.2 to 1.6 microm are demonstrated, and a route for substantial energy scaling is indicated.

Journal Article↗
PubMed2005

Controlling two-center interference in molecular high harmonic generation.

We experimentally investigate the process of intramolecular quantum interference in high-order harmonic generation in impulsively aligned CO2 molecules. The recombination interference effect is clearly seen through the order dependence of the harmonic yield in an aligned sample. The experimental results can be well modeled assuming that the effective de Broglie wavelength of the returning electron wave is not significantly altered by the Coulomb field of the molecular ion. We demonstrate that such interference effects can be effectively controlled by changing the ellipticity of the driving laser field.

Journal Article↗
PubMed2005

Measurement of harmonic phase differences by interference of attosecond light pulses.

By using a self-referencing technique, we have experimentally measured the influence of the carrier-envelope phase of femtosecond light pulses on the phase of the electric field of the radiation produced by high-order harmonic generation. We show that, in particular experimental conditions, the temporal evolution of the electric field of the attosecond pulses, is directly controlled by the carrier-envelope phase of the driving pulses.

Journal Article↗
PubMed2004

Observation of carrier-envelope phase phenomena in the multi-optical-cycle regime.

So far the role of the carrier-envelope phase of a light pulse has been clearly experimentally evidenced only in the sub-6-fs temporal regime. Here we show, both experimentally and theoretically, the influence of the carrier-envelope phase of a multi-optical-cycle light pulse on high-order harmonic generation. For the first time, we demonstrate that the short and long electron quantum paths contributing to harmonic generation are influenced in a different way by the pulse carrier-envelope phase.

Journal Article↗
PubMed2004

Effects of extender composition, cooling rate and freezing on the fertilisation viability of spermatozoa of the Pacific oyster (Crassostrea gigas).

The aim of this research was to optimise protocols for freezing spermatozoa of the Pacific oyster. All the phases of the cryopreservation procedure (choice of cryoprotectant, cooling, freezing, and thawing) were studied in relation to the species of spermatozoa to restore on thawing the morphological and physiological characteristics of fresh semen. The choice of type and concentration of cryoprotectant in which semen is incubated before freezing is fundamental for a successful cryopreservation: the cryoprotectants (dimethylsulfoxide--Me(2)SO, ethylene glycol--EG, propylene glycol-PG, and glycerol in concentrations between 5 and 15%) were tested for their toxicity on the semen exposed up to 30 min at +26 degrees C (room temperature) by evaluating its ability to fertilise and the embryo development to the regular D larval stage. The best cryoprotectants, Me(2)SO, EG, and PG 5, 10, and 15% respectively, were used for the pre-cooling (adaptation/cooling) tests. Two different adaptation/cooling procedures were tested: (A) from +26 degrees C to 0-2 degrees C (2.6 degrees C/min) and (B) at +26 degrees C for 15 min. Lastly, using the cryoprotectants and the adaptation procedure (B) that had given the best results in the preceding stages of the experiment, four cooling rates were tested: 6, 11, 16, and 21 degrees C/min. It was seen that the semen that was incubated with EG 10%, adapted at +26 degrees C for 15 min, and then cooled at a rate of 6 degrees C/min showed a percentage of regular D larvae on thawing comparable to that of fresh semen (p > 0.05).

Animals↗
PubMed2003

Above-threshold ionization at the few-cycle limit.

Photoelectron spectra measured for rare-gas atoms ionized with intense few-cycle laser pulses are presented. Several aspects of the few-cycle regime are discussed. In particular, the persistence of the plateaulike structure of spectra for high electron energies is shown. In contrast, a resonancelike feature at similar electron energies is suppressed as compared with longer laser pulses. Differences in the behavior of different species and implications for the electron-ion scattering cross section are pointed out.

Journal Article↗
PubMed2003

Generation of 3.8-fs pulses from adaptive compression of a cascaded hollow fiber supercontinuum.

We demonstrate generation of 3.8-fs pulses with energies of up to 15 microJ from a supercontinuum produced in two cascaded hollow fibers. Ultrabroadband dispersion compensation was achieved through a closed-loop combination of a spatial light modulator for adaptive pulse compression and spectral-phase interferometry for direct electric-field reconstruction (SPIDER) measurements as feedback signal.

Journal Article↗
PubMed2003

Single-shot kilohertz characterization of ultrashort pulses by spectral phase interferometry for direct electric-field reconstruction.

We describe a method of characterizing ultrashort optical pulses that is based on the technique of spectral phase interferometry for direct electric-field reconstruction and is capable of simultaneously measuring the amplitude and the phase of the electric field of a sub-10-fs pulse at kilohertz acquisition rates on a single-shot basis. Use of this technique results in a dramatic increase (>50x) in acquisition rate compared with that of existing diagnostics for full E-field characterization and opens the door to a range of new experiments in which shot-to-shot phase and amplitude fluctuations are studied at kilohertz rates.

Journal Article↗
PubMed2002

Time-resolved charge carrier generation from higher lying excited states in conjugated polymers.

Sub-ps three-pulse transient differential transmission spectroscopy using two excitation pulses is used to directly investigate the generation of charge carriers in ladder-type poly(para)phenyl in bulk film. The role of higher excited singlet states of both even and odd symmetry is examined and the dynamics of the major processes involved is described quantitatively. The charge generation efficiency is found to depend strongly on the delay between the two excitation pulses. This is explained by the interplay between internal conversion, excitation energy migration, and on-site vibronic relaxation.

Journal Article↗
PubMed2002

High-brightness high-order harmonic generation by truncated bessel beams in the sub-10-fs regime.

Low-divergence, high-brightness harmonic emission has been generated by using a fundamental beam with a truncated Bessel intensity profile. Such a beam is directly obtained by using the hollow-fiber compression technique, which indeed allows one to optimize both temporal and spatial characteristics of the high-order harmonic generation process. This is particularly important for the applications of radiation, where extreme temporal resolution and high brightness are required.

Journal Article↗
PubMed2001

A simple agar plate assay for screening siderophore producer yeasts.

Yeasts produce hydroxamate-type siderophores (iron-binding compounds) in response to Fe-stress conditions. Because these siderophores are important to the biocontrol of postharvest diseases of apple and pears, a method for screening siderophore producer yeast was developed. The screening method was carried out in special Petri dishes with eight or nine wells (25-mm diameter). These wells were filled with siderophore production medium and seeded with yeasts isolated from epiphytic apple microflora. After yeasts grew (24-48 h), holes (2-mm diameter) were made in the agar of each well. Holes were filled with an acid solution of ferric perchlorate. After 10-15 min, reddish halos appeared in the bottom of the plate and their intensities were compared with standards. Standards were prepared in the same special dish with rhodotorulic acid solutions (concentrations between 0.05 and 1 g/l) plus 2% agar. When agar solidified into wells, holes were made and filled with ferric perchlorate solution. Color intensities of reddish halos were proportional to siderophore concentration and the detection limit was 0.1 g/l. It was possible to correlate the production of siderophore in solid medium with the results obtained in liquid medium. The methodology was also a useful tool for making a preliminary assessment of the influence of different factors on the siderophore production.

Bacteria↗
PubMed2001

Effect of nitrogen source and pH on siderophore production by Rhodotorula strains and their application to biocontrol of phytopathogenic moulds.

The production of rhodotorulic acid, a siderophore synthesized by Rhodotorula strains, was improved with the objective of achieving the biocontrol of phytopathogenic moulds. Rhodotorulic acid increased up to 60% in the presence of urea as a nitrogen source, pH near to 8 and a C:N ratio of 8:1. The siderophore-containing spent medium showed in vitro antifungal activity against important plant pathogens including Botrytis cinerea, which causes grey mould on a wide variety of host plants including numerous commercial crops. The antifungal activity was related to siderophore concentration.

Amino Acids↗
PubMed2000

Storage of buffalo (Bubalus bubalis) semen.

Characteristics of buffalo semen, diluents used for liquid storage, aspects involved in freezing and thawing of semen are reviewed, and fertility results after artificial insemination (AI) with frozen-thawed semen are given.

Animals↗
PubMed2000

Effect of differential addition of glycerol and pyruvate to extender on cryopreservation of Mediterranean buffalo (B.bubalis) spermatozoa.

The composition of the extender in which semen is diluted before freezing plays a major role in successful cryopreservation of spermatozoa. Substances of high osmolarity, like glycerol, protect sperm cells during the freezing process and energy-rich compounds, like pyruvate provide extra energy during capacitation and fertilization. Since cryopreservation procedures for Buffalo spermatozoa have not been adequately defined, the aim of the study was to improve the survival rate of buffalo (Bubalus bubalis) spermatozoa after cryopreservation by optimizing the timing for adding glycerol and by enriching the cryoprotectant extender with an energy source substrate. Semen was collected with an artificial vagina from 5 bulls and the ejaculates were immediately evaluated for motility, forward progressive motility and for viability, pooled and held at room temperature (28 degrees C) for 1 h. Then aliquots of pooled semen were subjected to dilution and equilibration in triplicate as follows: Experiment 1. Glycerol (3%) in a commercial extender was added to the semen at 28 degrees C and cooled to 5 degrees C for 1 h; then extender with 11% glycerol was added before further equilibration (initial glycerol addition; IGA) and the samples held at 5 degrees C for 1, 3 or 5 additional hours (IGA 1, n = 24; IGA 3, n = 24; IGA 5, n = 24) before freezing. Experiment 2. Glycerol (3%) was added and the mixture brought to 5 degrees C as described above. Then extender with 11% glycerol was added (late glycerol addition; LGA) and after equilibration for 1, 3 and 5 h (LGA 1, n= 24; LGA 3, n = 24; LGA 5, n = 24) the samples were frozen. In Experiments 3 and 4 Na pyruvate (1.25 mM) was added to the extender as described for IGA and LGA above (IPA and LPA samples). The effect of addition time (initial vs late) of glycerol and pyruvate was evaluated by measuring sperm motility, progressively forward motility and viability. After freezing-thawing the percentage of motile spermatozoa was significantly higher (0.001<P<0.01) after a late addition of glycerol and pyruvate (LGA 5 and LPA 5). The optimizing of the timing of the glycerol addition and the presence in the extender of an energy source rendered a higher efficiency in thawed spermatozoa.

Animals↗
PubMed2000

Acute effects of rapamycin on glomerular dynamics: a micropuncture study in the rat.

The acute effects of cyclosporin (CsA, 20 mg(kg i.v.) and rapamycin (RAPA, 5 mg(kg i.v.) on glomerular dynamics were separately investigated by renal micropuncture in two groups of intact rats (group CsA and RAPA, respectively) and compared with vehicle-treated rats, used as controls (group CON). Left kidney glomerular filtration rate (GFR) was decreased by CSA (-35% vs. CON, P<0.05), but was not affected by RAPA (-14% vs. CON, NS), whereas the single-nephron GFR (SNGFR) was significantly decreased in both groups (-40% in CsA, P<0.01 and -26% in RAPA, P<0.05 vs. CON). In both groups glomerular plasma flow (GPF) was significantly reduced vs. CON (CsA: -48%, and RAPA: -25%) due to the increase in both afferent (Ra) and efferent (Re) glomerular resistances: group CSA showed a prevalent rise in Re (+98% vs. CON, P<0.001) than in Ra (+66%, P<0.001); in group RAPA the increment was modest and similar in Ra and Re (+33 and +32%, respectively, NS versus CON). A further group of rats was studied in which L-Arginine (ARG), the precursor of nitric oxide (NO), was administered (2.5 mg/Kg/min iv) with RAPA (group ARG). ARG limited the rise in Ra and Re, thereby preserving GPF; nevertheless, SNGFR remained low (-26% vs. CON, P<0.05) due to the decrease in the effective filtration pressure (-26% vs. CON). These data demonstrate that: (1) CsA is nephrotoxic at immunosuppressive doses; (2) RAPA, even at huge doses, has marginal effects on renal and glomerular dynamics; (3) the ARG-NO pathway is only partially involved in the vasoconstriction of superficial nephrons after RAPA administration.

Animals↗