Biomedical subjects
Publications and source records attributed to G Scarlata.
The incorporation into agar medium of either histidine or various buffers affects the colony size and the type of superficial outgrowth (fried egg), stabilizing the initial low pH of the medium.
Complement-fixing activity of a T strain of Mycoplasma was found to be associated with its lipid components. Heat stability and lability of periodate and beta-glucosidase treatments led to the conclusion that complement-fixing, active lipids had carboydrate determinants. Periodate treatment of chromatographic fractions of a chloroform-methanol extract showed that only antigens contained in the acetone fraction were periodate labile. Lipids also appeared to be involved in the passive hemagglutination, since the lipid fraction active in complement fixation also combined and blocked the action of antibodies which agglutinated sensitized erythrocytes with strain P108.
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In 1984-87, 10 isolates of Salmonella enterica subsp. bongori ser. 48:Z35:-, 9 of human source, were identified at the Southern Italy Centre of Enterobacteriaceae. This serotype had never been identified in Southern Italy before 1984. The combined use of different typing methods, with particular reference to restriction enzyme fingerprinting of plasmid and chromosomal DNA, supports the hypothesis that all Bongor serovars derive from a single strain.
The principal components (PC) analysis of standardized Rf values in four eluent systems [ethyl acetate/methanol/30% ammonia (85:10:15), cyclohexane/toluene/diethylamine (65:25:10), ethyl acetate/chloroform (50:50), and acetone with the plate dipped in potassium hydroxide solution] and of gas chromatographic retention indices in SE 30 for 277 compounds provided a two-principal-components model that explains 82% of the total variance. The scores plot allowed identification of unknowns or restriction of the range of inquiry to very few candidates. Comparison of these candidates with those selected from another PC model derived from TLC data only allowed identification of the drug in all the examined cases.
The retention factor of 54 drugs in eight eluent mixtures is reported. Principal component analysis (PCA) of these data provided a significant two-components model. These two parameters characteristic for each drug allowed an objective identification of unknown samples, provided they were included in the considered set. The analysis showed that the eluent mixtures cluster into three groups. The PCA model, using only three eluents (one for each group), was also able to restrict the range of inquiry to a few "candidates" and, in some cases, to allow unambiguous identification of the drug. These results, based on a simple and quick analytical determination (thin layer chromatography) and a reliable statistical procedure (PCA), appear to be of significant practical importance in the field of analytical toxicology.
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