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Biomedical subjects

G Scassellati Sforzolini

Publications and source records attributed to G Scassellati Sforzolini.

At least 19 recordsLinked to original sources

[Not Available].

The quality of drinking water is known to have positive or negative influences on human health. At present, in the industrialized countries microbiological contaminants in public water supplies are under control, due to the widespread introduction of effective disinfection and potabilization systems. However, of special interest are other public health problems linked with the presence of many water components or contaminants. Since the beginning of this century, fluoride showed to have beneficial effects on dental caries and the practice of adding fluoride to drinking water has been widely followed in many countries for reduction of dental caries. More recently, a large body of scientific information indicates that various characteristics related to water hardness are correlated with incidence of cardiovascular disease. Moreover, the discovery of the presence in drinking water of inorganic and organic contaminants with mutagenic/carcinogenic properties gives rise to public health concern.

Beverages↗

Monitoring nitrite, N-nitrosodiethanolamine, and mutagenicity in cutting fluids used in the metal industry.

We carried out an integrated environmental/biological monitoring program to evaluate cancer hazards among metal industry workers exposed to cutting fluids. Several cutting fluids were sampled according to response to a semiquantitative nitrite rapid test in metal factories in central Italy. The nitrite-positive samples were analyzed for nitrite and nitrosodiethanolamine (NDELA) content and mutagenic activity. The nitrite-negative samples were analyzed only for mutagenicity. Of the total samples, 20.6% were nitrite positive, and all contained NDELA. However, nitrite content was not quantitatively predictive of the NDELA content, which varied enormously among samples (0.3-1900 mg/kg). Nitrite-negative samples were always nonmutagenic. Mutagenicity was found in half the NDELA-containing samples but was not related to nitrite or NDELA content. Nitrite screening of cutting fluids in the field is an interesting method for identifying samples that potentially contain NDELA and other unknown mutagens and, when performed with short-term mutagenicity tests, nitrite screening seems to be a valid tool by which industrial managers and health officers could minimize the health hazards associated with occupational exposure to cutting fluids.

Diethylnitrosamine↗

Urine mutagenicity and biochemical parameters as markers of exposure to petroleum pitch using a rat model.

A petroleum pitch sample collected in a carbon electrode factory was studied using a series of in vivo assays for genotoxicity and enzymatic induction capability. Rats were treated with the petroleum derivative in three doses: 100, 50, and 10 mg/kg body weight. The treatment produced a rapid excretion of mutagenic substances in the urines of the first 24 hr only in rats treated with high doses (100 and 50 mg/kg). No faecal mutagenic activity was observed. Analyses of urinary thioethers showed that urinary metabolites derived from the compounds present in the pitch-sample at the lowest dose-administered (10 mg/kg) were eliminated primarily as cysteine conjugates. The pitch sample was found to be a good inducer of pulmonary and hepatic aryl hydrocarbon hydroxylase, especially after a 50 mg/kg dose. Urinary D-glucaric acid content was always statistically increased in treated animals compared with controls, confirming the enzymatic induction activity. Hepatic glutathione-S-transferase activity increased following treatment with 50 and 10 mg/kg doses.

Animals↗

Thioethers, mutagens, and D-glucaric acid in urine of operating room personnel exposed to anesthetics.

Mutagenic hazards related to occupational exposure to nitrous oxide and enflurane was studied in the personnel of five operating rooms using a coupled environmental and biological monitoring approach. The environmental monitoring revealed air concentrations of the two anesthetics exceedings the TLVs by 10-15-fold. These values were correlated individually with the concentrations of the two anesthetics in the expired air of the exposed subjects. The biological monitoring was carried out by determining two parameters associated with mutagen exposure (urinary mutagenicity and thioethers) and a parameter associated with the enzymatic induction (D-glucaric acid) in the urine of exposed and unexposed subjects (N = 64 and N = 37, respectively). The results showed no difference in the two groups for urine mutagenicity and D-glucaric acid, but urine thioethers were significantly increased among highly exposed subjects.

Adult↗

Urinary excretion of mutagens, thioethers and D-glucaric acid in workers exposed to bitumen fumes.

The authors carried out biological monitoring of the mutagenic/carcinogenic hazards associated with exposure to bitumen fumes during paving operations, analysing some biological parameters in the urine of a group of exposed workers. The urine samples were studied for mutagenicity by the Ames test and for thioethers concentration. D-Glucaric acid urine excretion was also determined to investigate the enzymatic induction potential of bitumens. Even though, in a previous environmental monitoring phase, a low content of mutagenic/carcinogenic compounds was found in bitumen and air samples, urinary mutagenicity data of exposed workers were statistically higher than those of a group of unexposed subjects. The urinary mutagenicity increased further if exposure to bitumens was associated with cigarette smoking. Thioethers were higher only in subjects exposed simultaneously to bitumens and cigarettes. D-Glucaric acid excretion did not increase significantly. The authors think that this type of coupled environmental and biological monitoring is a valid tool for a better evaluation of the mutagenic/carcinogenic exposure to bitumens or similar complex mixtures.

Air Pollutants, Occupational↗

Environmental monitoring of mutagenic/carcinogenic hazards during road paving operations with bitumens.

Environmental monitoring of mutagenic/carcinogenic hazards associated with occupational exposure to bitumen fumes was performed during road paving operations. Bitumen samples were collected and analysed for polycyclic aromatic hydrocarbons (PAH) content by HPLC and for mutagenicity by the Ames test. The exposure of sixteen road workers to bitumen fumes was studied. Time-weighted average values of bitumen fumes were determined by personal samplers. PAH concentration in the air and the mutagenicity of airborne particulates were also analysed. The results showed that bitumen samples contained low levels of total PAH (microgram/g) and were not mutagenic. Environmental monitoring showed a low level of exposure to bitumen fumes, which were found to contain only trace levels of PAH and not to be mutagenic. The authors suggest that these workers' exposure to mutagenic/carcinogenic agents is low.

Air Pollutants↗

Enzyme induction in rat lung and liver by condensates and fractions from main-stream and side-stream cigarette smoke.

Aryl hydrocarbon hydroxylase (AHH) and dimethylnitrosamine demethylase (DMND) activities in pulmonary and hepatic tissues of male Sprague-Dawley rats were assayed following pretreatment with known inducers (benzo[a]pyrene, 3-methylcholanthrene, Aroclor 1254, phenobarbital) and with main-stream (MS) and side-stream (SS) cigarette smoke condensates and their related fractions. Biochemical assays by spectrophotofluorimetry (AHH activity) and spectrophotometry (DMND activity) and by a biological assay (Ames test) were performed to detect AHH and DMND induction. Ames test proved to be much less sensitive than the spectrophotofluorimetric analysis for AHH determination. Both main-stream and side-stream cigarette smoke condensates and some fractions, containing water-soluble bases, water-insoluble bases, and polycyclic aromatic hydrocarbons, were found to induce AHH activity in lung and liver, the lung being induced to the greatest extent. The highest levels of AHH inducibility were found for the SS-smoke condensate and related fractions. In particular, the insoluble bases fractions gave the highest induction. On the contrary, pulmonary DMND activity was not affected by pretreatment with the same materials, while hepatic DMND response was only minimally induced by Aroclor and phenobarbital treatment.

Animals↗

Mutagenic/carcinogenic hazards in a cold-rolling steel plant exposed to mineral oils: environmental monitoring phase.

A survey was carried out in a cold-rolling steel plant exposed to mineral oils to study the mutagenic/carcinogenic hazards, following a coupled environmental/biological monitoring. The present paper deals only with the environmental phase by determining polycyclic aromatic hydrocarbons (PAH) and applying a mutagenicity test (Ames test), and includes the following steps: (a) work process and environment study; (b) sampling and analysing mineral oils before and after use (PAH determination and mutagenicity analysis); (c) sampling and analysing oil mist (TWA determination, PAH determination and mutagenicity analysis). The results showed that: (1) both unused and used (recycled) mineral oils contained only trace amounts of PAH and were not mutagenic; (2) the TWA concentrations of oil mists were lower than the TLV (less than 5 mg/m3); (3) oil mists contained only trace amounts of PAH and were not mutagenic. The authors suggest that these results could be due to the moderate temperature of the oil during the work process and that there is a relationship between low PAH content and absence of mutagenicity in the oils and air samples.

Air Pollutants, Occupational↗

Micronucleus induction in cells co-exposed in vitro to 50 Hz magnetic field and benzene, 1,4-benzenediol (hydroquinone) or 1,2,4-benzenetriol.

The generation, transmission (e.g. power lines, transformers, service wires, and electrical panels), and use (e.g. home appliances, such as electric blankets, shavers, and televisions) of electrical energy is associated with the production of weak electric and magnetic fields (EMF) which oscillate 50 (Europe) or 60 (USA) times per second (power-line frequency), falling in the extremely-low frequency (ELF) region of the electromagnetic spectrum. Epidemiological reports suggest a possible association between exposure to ELF-EMF and an increased risk of cancer (e.g. childhood acute leukaemia). Benzene is an established human leukomogen. This xenobiotic, which is unlikely to be the ultimate carcinogen, is metabolized in the liver to its primary metabolite phenol, which is hydroxylated to hydroquinone (1,4-benzenediol) and 1,2,4-benzenetriol. In this in vitro approach, to test the genotoxic and / or co-genotoxic potency of ELF-EMF, the cytokinesis block micronucleus (MN) method with Jurkat cells has been used. A 50 Hz magnetic field (MF) of 5 mT field strength was applied for different length of time (from 1 to 24 h), either alone or with benzene, 1,4-benzenediol, or 1,2,4-benzenetriol. Our preliminary results show that, after 24 h exposure, the frequency of micronucleated cells in MF-exposed cultures is 1.9 fold higher than in sham-exposed (control) cultures. Benzene exposure does not show any cytogenetic activity, whereas 1,4-benzenediol or 1,2,4-benzenetriol alone significantly affect the number of MN in Jurkat cells, as compared to untreated cultures. Moreover, co-exposure to ELF-MF does not seem to affect the frequency of micronuclei induced by benzene, 1,4-benzenediol, or 1,2,4-benzenetriol.

Benzene↗

[Obstetric care in cardiopathic pregnant women].

Heart diseases in pregnancy represent the main not-obstetrical cause of death and the fourth in absolute way. Cardiopathies should be diagnosed before pregnancy also to try a possible correct contraception. After discussing the modifications of the cardiovascular apparatus in a healthy pregnant subject, the authors dwell upon the modifications affecting heart patients in pregnancy. Particular attention is given to the therapeutical interruption in pregnant cardiopathic patients.

Female↗

[Renal physiopathology in pregnancy].

The authors examined the main anatomo-functional modifications of kidneys during pregnancy, through the analysis of "flux" and glomerular and tubular "functions". Among the modifications occurring in pathological conditions they included pregnant patients with only one kidney, patients affected by kidney calculosis, anomalies of urinary tract and patients who underwent kidney transplant.

Female↗

[Epidemiology and clinical presentation of vulvar cancer].

Recently, we observed a lowering in the average age of women affected by vulvar cancer. Five years after the diagnosis, survival is little more than 50%. Such a severe prognosis is caused both by a late diagnosis and organization lacks of prevention. Etiology and symptomatology of vulvar cancer are widely treated; particular prominence is given to the differential diagnosis.

Female↗