Biomedical subjects

G Schouten

Publications and source records attributed to G Schouten.

5 recordsLinked to original sources

PubMed2001

The human cell line PER.C6 provides a new manufacturing system for the production of influenza vaccines.

Influenza viruses for vaccine production are currently grown on embryonated eggs. This manufacturing system conveys many major drawbacks such as inflexibility, cumbersome down stream processing, inability of some strains to replicate on eggs to high enough yields, and selection of receptor-binding variants with reduced antigenicity. These limitations emphasize the need for a cell line-based production system that could replace eggs in the production of influenza virus vaccines in a pandemic proof fashion. Here we present the efficient propagation of influenza A and B viruses on the fully characterized and standardized human cell line PER.C6.

Animals↗

PubMed1999

Yoghurt enriched with Lactobacillus acidophilus does not lower blood lipids in healthy men and women with normal to borderline high serum cholesterol levels.

OBJECTIVE: To investigate whether intake of Lactobacillus acidophilus strain L-1 lowers serum cholesterol in healthy men and women. DESIGN: Randomised, placebo-controlled parallel trial. SETTING: Subjects were free-living. Blood sampling and distribution of yoghurts were administered at a local hospital. SUBJECTS: Seventy-eight adult men and women with cholesterol levels of 3.9-7.8 mmol/L (mean +/- s.d., 5.4+/-0.7). INTERVENTIONS: Subjects consumed 500 mL of control yoghurt daily for two weeks. They were then randomly allocated to receive 500 mL per day of control yoghurt or of yoghurt enriched with Lactobacillus acidophilus L-1 for another six weeks. The yoghurts were spiked with a trace of lithium; compliance as assessed by plasma lithium was excellent. RESULTS: Energy and nutrient intake was constant, and identical for the two groups. Mean body weight was stable. Baseline blood lipid concentrations in the control and treatment groups were highly similar. The effect of consumption of Lactobacillus acidophilus L-1 vs. control on total cholesterol was -0.02 mmol/L (95% CI, -0.18-0.15) after three weeks and 0.04 mmol/L (95% CI, -0.12-0.20) after six weeks. Serum LDL and HDL-cholesterol and triacylglycerol levels were also unaffected. CONCLUSIONS: Yoghurt enriched with Lactobacillus acidophilus L-1 does not lower serum cholesterol in men and women with normal to borderline high cholesterol levels.

Adolescent↗

PubMed1995

Brightness constancy in a Ganzfeld environment.

In this paper, experiments are described in which brightness constancy was studied in a Ganzfeld environment. Luminance variation by means of neutral density filters was applied to stimuli consisting of a Ganzfeld with superimposed disks. To this end, a special-purpose apparatus was constructed. Sequential dichoptical brightness matches with a reference stimulus were carried out for the disks as well as the homogeneous surround. The results of these measurements indicate that (1) besides a clear tendency toward brightness constancy, small but systematic effects of the average luminance level are present and (2) the brightness of the Ganzfeld is hardly affected by the presence of the disks. Finally, it is shown that the experimental results can be modeled adequately in terms of a concept that involves an accumulation of contrast information.

Color Perception↗

PubMed1995

Brightness indention: a novel compression mechanism in the luminance-brightness mapping.

In a previous study, Schouten and Blommaert (1995) explicitly showed that brightness constancy implies a substantial compression in the luminance-brightness mapping. Here it is argued that additional compression mechanisms are required for scenes with large luminance ranges. To trace these, a series of experiments was conducted which involved expansion of the luminance range. We used a simple spatial configuration consisting of a disk on a contourless homogeneous surround (Ganzfeld). The contrast between the disk and Ganzfeld acted as the independent variable, while the size of the disk was parametrically varied. Sequential dichoptical brightness matches with a reference were carried out for both the disk and the surround. The results reveal a compression mechanism which we term brightness indention. This indention, which has not previously been reported in the literature, only occurs if the Ganzfeld is less luminous than the disk, and it takes the form of a brightness decrease of the immediate surroundings of the disk.

Humans↗

PubMed1992

Maturational gonadotropin from the African catfish, Clarias gariepinus: purification, characterization, localization, and biological activity.

A gonadotropic hormone of the African catfish, Clarias gariepinus, was was purified and chemically characterized. Its biological activity was tested and its localization in the gonadotropic cells of the pituitary demonstrated. An ethanolic extract of 500 pituitaries of adult male and female African catfish was subjected to ion-exchange chromatography on DE-52. The 31- to 38-kDa fraction was further purified on Sephadex G-75. On rpHPLC over an ODS 120T column two major components appeared as single bands after SDS-PAGE. From the amino acid composition and sequence analysis of these fractions, compared with those of salmon and carp GTH II-alpha and salmon GTH II-beta it was concluded that they represent catfish GTH alpha- and II-beta-subunits. The biological activity of the complete hormone (the 31- to 38-kDa fraction from the G-75 column) was tested on the production of 11 beta-hydroxyandrostenedione and 17 alpha-hydroxy-20 beta-dihydroprogesterone by catfish testis in vitro. Polyclonal antibodies were raised against the purified beta-subunit. Immunocytochemical study using these showed them to bind specifically to hypophysial gonadotropic cells. To date only one form of GTH has been demonstrated in the African catfish.

Amino Acid Sequence↗