PubMed Health⌕ Search

Biomedical subjects

G Schwabe

Publications and source records attributed to G Schwabe.

15 recordsLinked to original sources
PubMed2003

Formoterol as relief medication in asthma: a worldwide safety and effectiveness trial.

The aim of the study was to compare the safety and effectiveness of as-needed formoterol with salbutamol in a large international real-life asthma study. Children and adults (n=18,124) were randomised to 6 months as-needed treatment with open-label formoterol 4.5 microg Turbuhaler or salbutamol 200 microg pressurised metered dose inhaler or equivalent. Primary safety variables were asthma-related and nonasthma-related serious adverse events (SAE)s and adverse events (AE)s resulting in discontinuation (DAE)s. The primary efficacy variable was time to first asthma exacerbation. The incidences of AEs, SAEs and DAEs arising from SAEs were not significantly different between treatments. DAEs for nonserious AEs were higher with formoterol. Asthma-related AEs decreased with formoterol (1,098 (12.3%) versus 1,206 (13.5%)), asthma-related SAEs were similar (108 (1.2%) versus 121 (1.4%)) but more asthma-related DAEs occurred in the formoterol group (89 (1.0%) versus 48 (0.5%)). Time to first exacerbation was prolonged (hazard ratio 0.86) and less as-needed and maintenance medication was used with formoterol. Reductions of exacerbations with as-needed formoterol versus salbutamol increased with increasing age and asthma medication level. This real-life study demonstrates that formoterol as-needed has a similar safety profile to salbutamol, and its use as a reliever therapy is associated with fewer asthma symptoms and exacerbations.

Administration, Inhalation↗
PubMed2001

Protection against cold air and exercise-induced bronchoconstriction while on regular treatment with Oxis.

This study aimed to compare the duration of protection against exercise-induced bronchoconstriction (EIB) after inhalation of formoterol (Oxis) Turbuhaler with that of terbutaline Turbuhaler and placebo Turbuhaler in asthmatic patients treated regularly with formoterol Turbuhaler 9 microg b.i.d. and inhaled steroids. The study. performed at three centres (Göteborg and Lund, Sweden, and Trondheim, Norway), consisted of an open-label part with formoterol Turbuhaler 9 microg b.i.d. and a randomized, double-blind, cross-over part with a single dose (on top of the regular treatment) of either formoterol Turbuhaler 9 microg, terbutaline Turbuhaler 0.5 mg or placebo Turbuhaler. The patients attended the clinic six times: twice for screening visits, three times for randomized treatment and once for a follow-up visit. Patients received regular b.i.d. treatment with formoterol 9 microg for a mean period of 16 days. Formoterol gave a post-exercise fall of 12, 10, 15 and 17% in forced expiratory volume in 1 sec (FEV1) 15 min, 4, 8 and 12 h after inhalation. The differences compared with placebo (falls of 26, 22, 23 and 22%) and terbutaline (falls of 17, 18, 22 and 22%) were all statistically significant (P<0.05 for all comparisons). Patients on regular treatment with formoterol Turbuhaler 9 microg b.i.d. have a significant protection against EIB up to 12 h after inhalation of formoterol 9 microg. The protection was also significantly better than that of terbutaline Turbuhaler 0.5 mg.

Adult↗
PubMed2000

Formoterol via Turbuhaler gave better protection than terbutaline against repeated exercise challenge for up to 12 hours in children and adolescents.

We aimed to compare the protective effect of single doses of 4.5 and 9 microg of formoterol fumarate (F), 0.5 mg terbutaline sulphate (T) and placebo (P), all via Turbuhaler, against exercise-induced bronchoconstriction (EIB) in children. Twenty-seven asthmatic children, showing a fall of > or =20% in FEV1 after a standardized exercise challenge test (ECT) combined with cold air (-10 degrees C) inhalation, were randomized in this cross-over, double-blind study. They had a mean age of 12.6 years (range 8-17 years), mean baseline FEV1 90% (73.9-105.6%) of predicted normal value. Seventeen children used inhaled glucocorticosteroids (120-750 microg day(-1)). ECTs were performed 15 min and 4, 8, and 12 h after drug administration. F significantly reduced the fall in FEV1 after ECT to 5.4% (15 min), 5.2% (4 h), 8.2% (8 h) and 9.3% (12 h) after 4.5 microg, and 2.5%, 3.0%, 5.0% and 5.4% after 9 microg, compared with a fall of 18.4%, 15.7%, 15.6% and 16.5% in FEV1 after P. The fall after T was 3.3%, 11.6%, 14.4% and 19.1% after 15 min, 4, 8 and 12 h respectively. The difference between F and T was statistically significant from 4 h and onward (P-value for all comparisons < 0.05). Children using a single dose of either formoterol Turbuhaler 4.5 or 9 microg had significantly better bronchoprotection against repeated exercise challenge up to 12 h compared with placebo and from 4 h onward compared with terbutaline Turbuhaler 0.5 mg.

Administration, Inhalation↗
PubMed1998

Centrotemporal spikes in families with rolandic epilepsy: linkage to chromosome 15q14.

OBJECTIVE: To localize a gene predisposing to benign epilepsy of childhood with centrotemporal spikes (BECTS). BACKGROUND: BECTS, or rolandic epilepsy, is the most prevalent idiopathic epilepsy syndrome in childhood. Functional relevant defects in the alpha 4 subunit of the neuronal nicotinic acetylcholine receptor (AChR) have been demonstrated in autosomal dominant nocturnal frontal lobe epilepsy, which, like BECTS, is an idiopathic partial epilepsy. METHODS: A DNA linkage study was conducted screening all chromosomal regions known to harbor neuronal nicotinic AChR subunit genes. Twenty-two nuclear families with BECTS were analyzed. RESULTS: In an "affected-only" study, best p values and lod scores were reached between D15S165 and D15S1010 on chromosome 15q14. In multipoint nonparametric linkage analysis a nominal p value of 0.000494 was calculated by GENEHUNTER. Best parametric results were obtained under an autosomal recessive model with heterogeneity (multipoint lod score 3.56 with 70% of families linked to the locus). These markers are localized in direct vicinity to the alpha 7 subunit gene of the AChR. CONCLUSIONS: We found evidence for linkage of BECTS to a region on chromosome 15q14. Either the alpha 7 AChR subunit gene or a closely linked gene are implicated in pedigrees with BECTS. The disorder is genetically heterogeneous. Surprisingly, the same chromosomal area has been reported to be linked to the phenotype in families with an auditory neurophysiologic deficit as well as in families with juvenile myoclonic epilepsy, another idiopathic but generalized epilepsy syndrome.

Adolescent↗
PubMed1993

Regulation of adenovirus 12 E1A transcription: E2F and ATF motifs in the E1A promoter bind nuclear protein complexes including E2F1, DP-1, cyclin A and/or RB and mediate transcriptional (auto)activation.

Nuclear factors NFI and NFIII are involved in transcription of the E1A oncogene of adenovirus 12. In addition, the E-box binding transcription factor ESF-1 was found to activate basal transcription from the proximal transcription start site TS2. Deletion of a region upstream from the distal start site TS1 was reported to abolish E1A transcription completely. Two motifs for transcription factors, one for members of the E2F family and one that was related to ATF motifs in the HTLV-1 LTR, are localized in this region. We examined the binding of nuclear proteins to these motifs and studied their role in (auto)activation of Ad12 E1A transcription from TS1. We found several cell type specific DNA-protein complexes in Electrophoretic Mobility Shift Assays (EMSA). For HeLa, 293, U937, and A549 cells, participation of E2F-1, DP-1, cyclin A, and RB was involved in formation of some complexes only, assuming participation of factors different from E2F-1 or DP-1 in others. One main and 2-3 minor specific complexes appeared in EMSA when the ATF-motif was examined. Partial cross-competitions occurred in competition experiments between the neighbouring E2F and ATF-motifs, suggesting cofactors or bridging proteins in formation or stabilization of some complexes. Transcription from TS1 mediated by these motifs was analysed using a CAT-reporter system, where neither the ATF- nor the E2F-motif alone imparted striking activation of transcription. In contrast, considerable and synergistic activation was observed when both sites were present in the CAT-construct. E1A autoactivation mediated by these sites was about twofold compared with a ninefold activation described for the complete E1A promoter.

Activating Transcription Factors↗
PubMed1990

Uptake of fluorescent plasmalogen analogs by cultured human skin fibroblasts deficient in plasmalogen.

One of the consequences of hereditary peroxisomal dysfunction in the cerebro-hepato-renal (Zellweger) syndrome (CHRS) is a dramatic decrease in the biosynthesis and cellular content of ether lipids. In the present study effects of reduced cellular plasmalogen levels on membrane-membrane interactions were investigated. Cultured CHRS fibroblasts were incubated with unilamellar phospholipid vesicles consisting of 1-O-alkenyl-2-acyl- or 1,2-diacyl-sn-glycerophosphocholines and ethanolamines, carrying either the trans-parinaroyl or the 1,6-diphenyl-1,3,5-hexatriene propionyl group in position 2. Transfer of the fluorogenic phospholipids from vesicles to cells was followed by measuring the concomitant increase in fluorescence intensity. Transfer of phospholipids from cells to vesicles was monitored by incubating cells, prelabeled with [3H]oleic acid, in the presence of phospholipid vesicles. Fibroblasts from healthy donors or CHRS fibroblasts supplemented with the plasmalogen precursor 1-O-hexadecylglycerol served as controls. Plasmalogen-deficient cells exhibited a significantly increased tendency to take up exogenous choline or ethanolamine plasmalogens. Cellular plasmalogens were transferred from control cells to vesicles at a higher rate if the acceptor vesicles consisted of plasmalogens as compared to diacylglycerophosphocholine. Thus, it appears as if mechanisms existed which preserve cellular plasmalogen levels during interaction with exogenous phospholipid pools. Preliminary experimental evidence suggests that the observed exchange of phospholipids between cultured fibroblasts and vesicles occurs by a protein-catalyzed process.

Biological Transport↗
PubMed1985

Restriction endonucleases in Azospirillum.

Azospirillum brasilense, A. amazonense, and A. lipoferum strains were screened for restriction endonucleases using phage lambda DNA. The extract of A. brasilense 29711 cleaved lambda DNA into specific fragments. It was concluded that this strain possesses a class II restriction endonuclease which was named AbrI. AbrI has a single recognition site on lambda DNA at position of approx. 33 500 bp. AbrI was characterized as an isoschizomer of XhoI, which cuts lambda DNA at 33 498 bp and cleaves double-stranded DNA at the sequence 5'-C TCGAG-3'. From other Azospirilla strains only A. amazonense QRZ42 extracts (AamI activity) cleaved DNA into specific fragments under certain conditions.

Bacteriophage lambda↗
PubMed1979

[The frieze of the "Mystery villa" in Pompeii. Contribution to the development of female psychology].

The large frieze of the "Villa of the Mysteries" at Pompeji is interpreted on the base of the psychology of C. G. Jung, especially of his student Erich Neumann. According to his psychological theories the frieze is depicting the development of the female principle to individuation, arranged with extraordinary artistic inspiration. Through the myth of Ariadne-Theseus-Dionysus the change of the woman, disappointed from the personal man and hero is shown. Through the experience of the transpersonal male principle in herself she is entering in a new level of conscience, the patriarchism, and so coming to the highest development, to the "Selbst". The experience of the male principle is made through Dionysus, a symbol of change in the theory of the "Archetypen" found by C. G. Jung.

Art↗