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Biomedical subjects

G Senaldi

Publications and source records attributed to G Senaldi.

88 records · Page 5Linked to original sources
PubMed1987

Activation of the alternative complement pathway: clinical application of a new technique to measure fragment Ba.

A new laser nephelometric technique for the measurement of the alternative complement pathway fragment Ba has been developed. Activation of the alternative complement pathway was assessed in 16 patients with Gram negative bacteraemia, six with Gram positive bacteraemia, 20 with rheumatoid arthritis, and 18 healthy subjects. Patients with Gram negative bacteraemia had significantly higher values of Ba (median 14.8%) than controls (9.3%) (p less than 0.01), while patients with Gram positive bacteraemia and rheumatoid arthritis had values similar to those of controls (10.1% and 9.5%). The technique proved sensitive and precise, and is suitable for the routine laboratory evaluation of complement activation through the alternative pathway.

Adolescent↗
PubMed1987

Peripheral blood lymphocyte subpopulations after splenectomy for trauma.

Absolute and percent numbers of peripheral blood lymphocyte (PBL) subpopulations were evaluated using monoclonal antibodies in 20 subjects splenectomised for trauma. A significant increase was observed in the absolute counts of total PBL, B, null and Leu 7+ cells and in the percent counts of null and Leu 7+ lymphocytes. The percent numbers of OKT11+, OKT4+ and OKT8+ lymphocytes were significantly reduced, but the OKT4+/OKT8+ ratio was not statistically different from normal controls. The increase in B and killer/natural killer cells may provide a compensatory mechanism responsible for surveillance against infections and tumors in splenectomised patients, despite the numerous humoral and cellular abnormalities they commonly display.

Adolescent↗
PubMed1986

Dynamic and ultrastructural studies of in vitro interaction between Toxoplasma gondii and cultured cell lines. I. Adhesion and cellular penetration.

Toxoplasma gondii is a protozoan parasite that requires an intracellular environment for growth and replication. This study describes the early phase of the interaction between the parasite and its host cell. With the aid of phase contrast microcinematography, it has been possible to observe adhesion to cell membrane, and the closely following intracellular penetration. This report also reviews the ultrastructural and biochemical grounds that could account for the infecting process.

Animals↗
PubMed1985

Characterization of circulating T-lymphocyte subsets in alcoholic liver disease by monoclonal antibodies: correlation with the absolute lymphocyte count.

Peripheral blood T-lymphocytes from 27 patients with alcoholic liver disease were assessed on the basis of their ability to react with OKT monoclonal antibodies. A significant positive correlation was observed between the absolute numbers of T-lymphocytes and suppressor/cytotoxic T cells and the absolute lymphocyte count. These data suggest that quantitative changes in T-lymphocyte subsets in alcoholic liver disease do not necessarily reflect an imbalance in T immunoregulatory mechanisms.

Antibodies, Monoclonal↗
PubMed1981

[Evaluation of the methylthymol blue and orthocresolphthalein direct methods of determination of serum calcium].

Two methods for the direct colorimetric determination of serum calcium (BMT and OCFT) have been evaluated and compared with atomic absorption spectrophotometry (AAS). Both colorimetric methods resulted simple to use, but temperature-sensitive: values for the ratio absorbance at 10 degrees C/absorbance at 45 degrees C were 1.6 (BMT) and 1.8 (OCFT). When readings were taken at 37 degrees C good precision was achieved with both methods (between-the-series coefficients of variation were 3.5 divided by 2.2% for OFCT and 1.9 divided by 3.4% for BMT). No interference from bilirubin (up to 180 mumol/l) was observed with both methods; slight positive interference from haemoglobin (1 g/l) was observed only with OCFT method. Results from the two colorimetric methods closely agreed in the range 1.0 divided by 4.5 mmol/l: statistical analysis of comparison data gave BMT = 0.0963 + 0.9521 OCFT, r = 0.9820. From the results of direct comparison with AAS (103 serum specimens, concentration spanning from 1.0 to 4.5 mmol/l) statistical analysis gave: BMT (y)/AAS(x): y = 0.0721 + 1.0226x (r = 0.9678); OCFT(y)AAS(x): y = -0.0208 + 1.0720x (r = 0.9836): it is concluded that both methods show a slight positive bias with respect to AAS.

Bromthymol Blue↗
PubMedDate not supplied

Serum beta 2-microglobulin levels in multiple myeloma and monoclonal gammapathy of undetermined significance: a clinical study of 55 patients.

Previous reports provided clear evidence that serum beta 2-microglobulin (beta 2m) is a marker highly correlated with the total mass of myeloma cells and suggested its use in the follow-up of patients with plasma cell tumors. Serum beta 2m levels were measured in 38 patients with multiple myeloma (MM), in 17 patients with monoclonal gammapathy of undetermined significance (MGUS) and in 32 normal control subjects. While statistically significant differences could be established between controls and both MM and MGUS patients, between patients with MM at stage III and patients with MM at lower stages as well as between patients with MGUS and patients with mostly advanced MM, it was not possible to statistically separate patients with MM at stage I from patients with MM at stage II, patients with untreated MM from patients with treated MM and, finally, patients with MGUS from patients with low cell mass MM. These results substantially confirm the already published data and lead to the conclusion that serum beta 2m determination is a useful test in the clinical management of monoclonal gammapathies, but it does not allow a differential diagnosis between benign and malignant forms of the disease.

Aged↗