Biomedical subjects
Publications and source records attributed to G Shambaugh.
1. Stimulation of brain cell growth by fetal plasma is reduced when levels of IGF-I are low and IGFBP-1 are high. 2. Fetal brain cells respond to IGFs in cell culture, and effects of plasma are reduced by addition of IGFBP-1. 3. Increased levels of IGFBP-1 and reduced availability of IGF-I may contribute to reduced brain growth when maternal nutrition is impaired.
TRH immunological and biological activities were found in extracts of human placentas. Eight term placentas delivered by cesarean section were extracted with 2 N acetic acid, followed by glacial acetic acid. Lyophilized samples were resuspended in phosphate-buffered saline. TRH activity, identified in each placental extract by specific RIA, averaged 19.8 +/- 3.3 pg/mg protein. TRH in the placental extracts was similar to synthetic TRH by four criteria. 1) Serial dilutions of placental extracts defined an immunoassay inhibition curve with a slope (-1.93) identical to synthetic TRH (-1.88). 2) When placental extract or synthetic TRH was chromatographed on Sephadex G-10, TRH immunoreactivity was found in similar fractions of the eluate. 3) Both placental extract and synthetic TRH stimulated TSH release from rat pituitaries in vitro. 4) Human serum degraded placental extract and synthetic TRH in a qualitatively similar manner. Placental minces did not accumulate exogenous [3H]TRH, suggesting that the placental TRH may originate endogenously.
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