Biomedical subjects
Publications and source records attributed to G Soslau.
A cell line resistant to ethidium bromide has been developed from baby hamster kidney cells transformed by Rous sarcoma virus. The parental cells are normally non-producers but the resistant cell line appears to produce virus-like particles that are associated with the mitochondria as visualized by electron microscopy and determined biochemically.
Recently we demonstrated that ethidium bromide altered the plasma and subcellular membrane glycoproteins in control and virus transformed cells. It is reported here that ethidium bromide also stimulated the membrane associated process of sugar transport. The KM of the virus transformed cells and the ethidium bromide treated cells is the same as that of the control cells while the maximum velocity as compared to the control cells is significantly increased. The transport of 2-deoxyl-D-glucose was inhibited by glucose, cytochalasin B and neuraminidase but was unaffected by variations in cell density or pH of the incubation medium.
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Ethidium bromide intercalates between the bases of native DNA, resulting in several biological anomalies. The effects of ethidium bromide on the mitochondria of cultured mouse L cells were studied. At a concentration of 1 microg ethidium bromide/ml it was observed that concentrations of cytochromes a + a(3) and b decreased, a + a(3) more rapidly than b. In contrast, the concentration of cytochromes c(1) and c increased or remained the same as in control cells. Concomitant with the decrease of cytochromes a + a(3) and b was an enlargement of the mitochondria and a reduction in the cristae. The cristae that remained were abnormally organized. After prolonged treatment with ethidium bromide a second population of small, more normally organized mitochondria was apparent. These effects of ethidium bromide could be reversed.