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Biomedical subjects

G Stauch

Publications and source records attributed to G Stauch.

At least 19 recordsLinked to original sources
PubMed1989

Effects of Nd-YAG laser and cryosurgery on the oral mucosa in rabbits.

In 32 rabbits, lesions of comparable depths were created in the oral mucosa using a Nd-YAG laser (Medilas II, 1.06 microns, MBB Co., Munich) and a cryosurgery unit (Kryo-S, Erbe Co., Hannover). Histologic specimens were examined between 1 h and up to 8 weeks following operation. The laser lesion was restricted horizontally to the laser-exposed area; the full extent of the lesion could already be seen after 1 day. Cryosurgical lesions showed their maximum extent 3 days following treatment. Wound healing processes following laser application, were accelerated compared to cryosurgical procedure.

Animals↗
PubMed1989

[The prognostic value of lymphangiosis carcinomatosa and malignancy grading in T1/T2-N0 cancers of the breast using 10 or fewer examined axillary lymph nodes].

In 270 cases of T1 and T2 carcinomas of the breast with ten or less axillary lymph nodes having been examined, the prognostic relevance of the histopathological statement of nodal negativity was assessed in a retrospective study. In this collective study, the mean number of lymph nodes examined was 4.1 +/- 1.9 for the 153 N0 carcinomas and 4.4 +/- 2.2 for the 117 cases with axillary lymph node metastases, respectively. The patients with N0 carcinomas were grouped according to the number of lymph nodes examined, thus resulting three subgroups, those with 1-2, 3-6, and 7-9 lymph nodes having been sampled and subjected to histopathologic evaluation. There was an approximately equal distribution in these three groups with respect to tumor size and tumor types; a correlation between the patients' age and the number of lymph nodes examined could not be found. A comparison of the three groups revealed conspicuous differences among the N0 carcinomas regarding both the disease-free survival time (DFS) and the overall survival time (OS), whereas a higher representativity of the determination of the nodal state, correlating with the number of lymph nodes examined, could not be confirmed. The disease-free survival time, the rate of disease recurrence as well as the median survival time and the 5-year survival rate, however, could be shown to be significantly dependent on tumor-related conditions, especially the grade of malignancy in connection with the presence or absence of peritumoral lymphatic or blood vessel invasion.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1988

[Comparison of raster mammography and real time sonography with histology in carcinoma of the breast].

In a retrospective study, the results of real-time sonography and grid mammography in 161 patients with histologically confirmed breast cancer were analyzed and compared with the histopathologic characteristics of the tumours to assess the diagnostic accuracy of these preoperative image-producing methods. The results showed the determination of the tumour size as obtained by the image-producing methods to correlate closely with the histopathologic findings. Furthermore, both real-time sonography and mammography yielded certain indications allowing prognostically valuable conclusions in respect of malignancy and invasiveness of the carcinomas. Taking into account the different but complementing diagnostic results of both methods, the combination of grid mammography and real-time sonography must be considered as the presently most reliable preoperative diagnostic procedure.

Breast Neoplasms↗
PubMed1988

[Determination of the growth fraction using monoclonal antibody Ki-67 in breast cancers].

In 329 breast tissues (120 benign lesions and 209 invasive breast carcinomas) the growth fraction was determined using the monoclonal antibody Ki-67. The percentage of Ki-67 positive cells or the Ki-67 growth fraction was determined on histologic and cytologic specimens after immunoperoxidase staining. In benign breast lesions, the Ki-67 growth fraction never exceeded 11%, whereas in breast cancer 14.4 +/- 9.9% of cells were Ki-67 positive ranging from 1 to 48%. Breast carcinomas were classified according to the histologic grading system of Bloom and Richardson (G1-G3). Poorly differentiated carcinomas. (G3) had significantly higher Ki-67 growth fractions than well differentiated tumors (G1). Ki-67 was also correlated with axillary lymph node status. Growth fractions of N+ carcinomas were significantly higher than those of N0 tumors (16.4 +/- 10.4% versus 12.4 +/- 9.4%; p = 0.006). Thus, this new method yields similar results to those obtained by other researchers through the use of flow cytometry and thymidine labelling. As determination of Ki-67 growth fractions is an easy and rapid technique, it is ideal for routine clinical use. Ki-67 may be useful in prognosis and in the selection of patients for various treatment modalities such as adjuvant therapy. A wide-spread use of Ki-67 would be helpful to gain further experience.

Antibodies, Monoclonal↗
PubMed1987

The correlation of growth fractions with histologic grading and lymph node status in human mammary carcinoma.

The monoclonal antibody Ki-67 reacts with a human nuclear associated with cell proliferation that is expressed only in the G1, S, G2, and M phases of continuously cycling cells. This offers a simple opportunity to determine the growth fraction of tumors by immunostaining of fresh tissue. One hundred fifty-four invasive carcinomas of the breast were used in this study. The average number of Ki-67 positive cells was 15.3 +/- 10.1% (range, 1%-48%), whereas in 41 benign lesions of the breast only 4.4 +/- 2.6% (range, 1%-10%) of cells were positive. A correlation was found between growth fractions and histologic grading. On average, N+ tumors with less than four positive lymph nodes had a significantly higher growth fraction (20.4 +/- 14.2%) than N0 tumors (13.0 +/- 9.2%), whereas N+ tumors with more than three lymph node metastases had only 17.3 +/- 3.6% Ki-67 positive cells. This method yielded similar results to those obtained by other researchers through the use of flow cytometry and thymidine labeling. Determination of growth fractions by Ki-67 is suitable for routine use and may be useful in prognosis and in the selection of patients for various treatment modalities.

Antibodies, Monoclonal↗
PubMed1987

Determination of growth fractions in benign breast disease (BBD) with monoclonal antibody Ki-67.

Certain types of benign breast disease (BBD) carry an increased risk of malignancy. Several morphological criteria such as an atypia score are used to define this group, and the use of a kinetic parameter may provide additional information. Therefore, the growth fractions of 120 benign breast lesions were determined using the monoclonal antibody Ki-67. The values obtained range from 0.3% to 10.8% (average 3.1% +/- 2.2%) compared to breast carcinomas with an average of 15.3% +/- 10.1%, range 0.8% to 47.8%. All specimens were classified using the terminology of Azzopardi. The prevailing histological entities were cystic disease, fibroadenoma, and blunt duct adenosis. Between these groups no differences in growth fractions were observed. Postmenopausal patients had slightly lower values than premenopausal women. Further prospective studies are needed to evaluate whether those cases of BBD with a high number of Ki-67 positive cells have an increased risk of breast cancer, independently from conventional histological classifications.

Adenoma↗
PubMed1986

[Mastopathy and breast cancer: is there a typical hormone profile?].

Carcinoma of the breast and mastopathy are statistically correlated with the functioning of the gonads. The authors studied whether in 58 women with carcinoma of the breast and in 36 patients with mastopathy any change was seen in the secretory pattern of gonadal steroids and whether there were any latent changes in the secretion of prolactin and TSH. A group of 43 women with benign gynaecological diseases served as controls. Before making the diagnosis via immediate section for microscopic examination we determined the basal values of prolactin, LH, FSH, TSH and the oestrogen fractions E1, E2, E3, total oestrogens and progesterone. In addition, we measured the increase in prolactin and TSH after stimulation with metoclopramide. Postoperatively this was followed by measuring the basal values of prolactin and TSH and their secretory performance in respect of TRH. All histological diagnoses were made by an experienced pathologist. Examinations were performed under strictly standardised conditions, taking into account stress, circadian variation and the nutritional status. The following group-specific significant results were obtained (p less than 0.05-0.01): Mastopathy: high serum values of all oestrogen fractions and of total oestrogens, high PRL release through MTC stimulation, prolactin secretory effect after MTC in accordance with the degree of severity of epithelial proliferation, early onset of menopause. Carcinoma of the breast: Low PRL and TSH release on MTC stimulation, low progesterone concentrations during the second cycle phase, relatively high serum concentrations of oestradiol during the first cycle phase, PRL basal values increased postoperatively, compared with preoperative values. The results led to the following conclusions: In mastopathy patients there is a connection between high oestrogen concentrations and consecutively high prolactin release. The morphological substrate is represented by an enhanced proliferation of epithelium. In patients with cancer of the breast reduced progesterone concentrations during the second half of the cycle lead us to suspect an increased incidence of luteal insufficiency; in addition, it appears that the daily prolactin secretion rate in this group is on a reduced level.

Adult↗
PubMed1986

Growth fractions in breast cancers determined in situ with monoclonal antibody Ki-67.

The growth fractions of 160 mammary carcinomas and 30 benign mammary lesions were determined in situ by immunostaining with the monoclonal antibody Ki-67. Benign lesions had a mean value of 3% Ki-67 positive cells, whereas the mean value of mammary carcinomas was 16.6%. A comparison of the mean values of Ki-67 positive cells with the histological grade of the tumours showed a correlation between these two variables--that is, histological grade 1 showed 9%, grade 2 16%, and grade 3 26% proliferating cells. Considering the individual Ki-67 values in the different histological grades, it was evident that there was considerable scatter in the number of proliferating cells, so that the proliferation rates of grades 1, 2, and 3 overlapped each other. This indicates a dissociation between histological grade of malignancy and size of the growth fraction in most breast cancers. Follow up studies are needed to establish which of the two variables--that is, morphological degree of malignancy, or the proportion of Ki-67 positive cells--correlates better with response to treatment and survival in individual cases.

Antibodies, Monoclonal↗
PubMed1985

[Endometrioses in a surgical patient sample].

In general surgery patients, endometrioses can occur in the gastro-intestinal tract; they can also appear as cicatricial endometrioses or can be manifested as other, rare localisations of endometriosis tissue. In many cases it is not possible to arrive at a pre-operative diagnosis which is histologically established. Hence, treatment in endometriosis of the colon is surgical resection in order to delineate it against carcinoma and for the purpose of treating cicatricial stenosis which is usually associated with endometriosis. In cicatricial endometrioses and cysts in the inguinal region, diagnosis became possible only after surgery and postoperative histological examination.

Adult↗
PubMed1984

Oncogenic activity of the BK type of human papova virus in inbred rat strains.

The oncogenicity of the human polyomavirus BK (BKV) was tested in newborn inbred rats. It was found that the tumor rate was negatively correlated with the levels of T antibody 3 months after inoculation and the frequency of animals with detectable T antibodies 1.5 months after inoculation. By contrast, no influence of viral HI titers on the tumor rates was found. Thymectomy of animals resulted in most experiments in increased tumor rates. Inoculation with BKV of animals later than 24 hours after birth yielded a decrease of tumor rates. The results obtained suggest that T antibody titers present at a critical time after inoculation are associated with low oncogenicity of BKV. The oncogenicity of BKV was comparatively tested in rat strains possessing the allele "l" or the allele "a", respectively. The oncogenicity was significantly higher in rats with the allele "l" than in rats with the allele "a". Rats with the allele "l" showed lower T antibody response than rats with the allele "a". These differences could be explained by the finding that cells of "a" origin showed in vitro a higher percentage of T antigen bearing cells than did cells of a strain possessing the allele "l". In comparison to previous results obtained with BKV inoculated outbred WISTAR rats, the oncogenicity of comparable BKV doses in inbred rats was generally higher and the latency period of tumor manifestation shortened.

Animals↗
PubMed1984

Distribution of polyoma virus in the organs of newborn Wistar rats after subcutaneous inoculation.

Polyoma virus of the Stuart Eddy strain was tritium-labelled by cultivating the virus in phosphate free Hepes buffered medium in presence of tritium labelled nucleotide triphosphates. The distribution of labelled virus in the organs and in the blood of newborn Wistar rats was examined at graded time intervals from two hours until 40 days after inoculation. The highest amounts of virus per organ was refound in the liver and the highest amount per mg organ weight in the spleen. The virus concentration in most organs exceeded by far the concentration found in the blood, indicating adsorption of virus in these organs. It was found that the distribution of tritium in the target organs for tumor appearance (kidneys and brain) did not differ basically from that in the non-target organs. As a consequence, the distribution of virus in the organs does not explain the fact that the kidneys and the brain are the predilection sites for tumor induction. Tritium labelling did not measurably influence the oncogenicity, infectivity and hemagglutinating activity of the virus.

Animals↗
PubMed1982

Aortic valve replacement and ascending aorta replacement in ankylosing spondylitis: report of three surgical cases and review of the literature.

Out of 887 consecutive patients who underwent aortic valve replacement between January 1976 and December 1981 at Hannover Medical School Hospital, 3 patients had severe aortic valve insufficiency associated with ankylosing spondylitis (Morbus Bechterew). One of them had huge aneurysmatic dilatation of the ascending aorta and successfully underwent replacement of the ascending aorta by a vascular prosthesis. Microscopical examination of the resected aortic wall showed characteristic findings of aortitis in ankylosing spondylitis. The 3 patients are in good clinical condition at 5 and 6 months, and 2 1/2 years, respectively, after uneventful surgery. It is concluded that aortic valve replacement in patients with ankylosing spondylitis can be performed feasibly and clinical results have been satisfactory. The risk of aneurysmatic dilatation of the ascending aorta resulting from aortitis associated with ankylosing spondylitis is emphasized.

Aorta↗