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Biomedical subjects

G Sturm

Publications and source records attributed to G Sturm.

At least 55 records · Page 3Linked to original sources
PubMed1983

Inhibition of progesterone secretion in cultured human granulosa cells by a low molecular weight fraction of human follicular fluid.

Previous studies demonstrated that a low molecular weight peptide fraction (G10-3) from human follicular fluid (FFl) could inhibit steroidogenesis by rat granulosa cells in vitro. In the present study this FFl fraction was tested upon human granulosa cells. The G10-3 fraction (less than 1000 mol wt) was obtained by sequential gel filtration on Sephadex G50 and G10 of a steroid-free extract of a pool of human FFl collected from various-sized follicles at different stages of the menstrual cycle. Human granulosa cells were obtained from large, healthy follicles in the mid- to late follicular phase of eight patients, and cultured for 4-6 days in the absence or presence of G10-3. In all cases G10-3 produced a dose-dependent inhibition of basal progesterone secretion and, when tested, also of FSH-stimulated progesterone secretion. Inhibition of progesterone secretion appeared to be greater in cells obtained from less mature follicles as compared to cells obtained from follicles that were periovulatory. The results suggest the presence of a low molecular weight luteinizing inhibitor in human FFl.

Body Fluids↗
PubMed1983

Evidence for glutathione-S-transferase activity in human blood platelets.

Data will be presented pointing to the presence of glutathione-S-transferase activity in human blood platelets and the possible involvement of this enzyme in the process of platelet activation. Using 1-chloro-2,4-dinitrobenzene as a synthetic substrate of the glutathione-S-transferase a rapid dose-dependent depletion of platelet glutathione was measured. The formed GSH-CDNB conjugate was separated by thin-layer chromatography. Hints to the formation of leukotriene-like substances by glutathione-S-transferase catalysed reaction were obtained using the specific leukotriene C antagonist FPL 55 712 in aggregation studies.

Arachidonic Acid↗
PubMed1983

Influence of epidural fentanyl on stress-induced elevation of plasma vasopressin (ADH) after surgery.

The role of techniques used for management of intra- and postoperative pain on plasma levels of antidiuretic hormone (ADH) was evaluated in 107 patients undergoing abdominal or thoracic surgery. Fifty-one patients had neurolept-anesthesia (NLA) intraoperatively followed by intramuscular piritramide, a long-lasting synthetic narcotic, for relief of postoperative pain. Fifty-six patients had a combination of epidural bupivacaine and NLA intraoperatively followed by epidural fentanyl for relief of postoperative pain. All patients had daily measurements of serum levels of potassium and sodium, plasma levels of ADH, and plasma osmolality for the first 5 postoperative days. In 67 patients arterial blood-gas tensions also were measured at similar times. There were no significant changes in serum electrolyte levels, plasma osmolality, or blood-gas tensions intra- or postoperatively. Plasma ADH levels increased postoperatively in all patients, but in patients given NLA followed by postoperative intramuscular narcotics, plasma levels of ADH were more than twice as great as in patients given epidural anesthesia followed by epidural fentanyl.

Adult↗
PubMed1982

Biochemical characterization of the layers of subcutaneous adipose tissue in the pig body.

During the formation of two layers of adipose tissue in the pig's body, starting from the 80th day after birth, samples were obtained by biopsy and analyzed for gross constituents and enzymes concerned with fatty-acid biosynthesis. These two layers differ in total lipid and water content and demonstrate more subtle differences amongst DNA, protein, collagen and sodium concentrations when comparisons are made in regard to age, sex, and breeding selection for low-fat animals. Acetyl-CoA carboxylase, malic enzyme and glucose-6-phosphate dehydrogenase are more active in the inner layer, while 6-phosphogluconate and isocitrate dehydrogenases are distinguishable in the two layers of adipose tissue as well if age, sex, and breeding line are taken into consideration. The data form the basis for a more detailed study of lipogenic potentials in adipose tissue (next paper).

Acetyl-CoA Carboxylase↗
PubMed1982

A study of acetyl CoA-carboxylase in adipose tissues.

Acetyl-CoA-carboxylase activities were measured in adipose tissues of pigs during a breeding experiment for a low-fat line, and of rats and obese mice under different nutritional conditions. Acetyl-CoA-carboxylase behaves uniformly with the four major NADPH-generating dehydrogenases, like a block of lipogenic enzymes, and is found to be genetically determined in pigs. Correlation with body fat under a variety of experimental conditions confirms the rate-limiting character of acetyl-CoA-carboxylase, not only for the biosynthesis of fatty acids, but obviously also for their esterification and for triglyceride deposition. Activity ratios of this enzyme in different adipose tissues, e.g. outer versus inner layer of subcutaneous adipose tissue in pigs, epididymal versus subcutaneous, or epididymal versus perirenal adipose tissue in rats and obese mice, correlate well with predicted fattening in pigs and with fat deposition in laboratory rodents. Moderate biotin deficiency in obese mice leads to a preferred fat deposition in the epididymal fat pad in comparison with normal biotin supply. The concept of a lipogenic potential in the body is derived from the activity ratios of acetyl-CoA-carboxylase.

Acetyl-CoA Carboxylase↗
PubMed1982

[Modified neuroleptanalgesia with buprenorphine].

A modification of neuroleptanalgesia by substituting fentanyl with buprenorphine is presented. Both anaesthesia techniques could be applied alternatively. We did not recognize any significant difference between the two groups concerning haemodynamics, the secretion of the so called stress hormones (antidiuretic hormone, cortisol) as well as the postoperative respiratory depression. The long lasting analgesia, which could be achieved by buprenorphine, can be advantageous in certain surgical interventions. The lack of a potent antagonist for buprenorphine in addition to its longer half-life for--not being of advantage in any anaesthesia--is discussed.

Adult↗
PubMed1982

Distribution of memantine in brain, liver, and blood of the rat.

The distribution of 1,3-dimethyl-5-aminoadamantane (DMAA, D-145, memantine, Memantine), a compound used in the treatment of CNS disorders, is investigated in liver, brain, and blood of the rat. The tissue concentration of memantine is analyzed by gaschromatography after i.p. and p.o. administration. After i.p. application the blood levels are only about 1/10-1/20 of the concentrations found in liver and brain. The highest drug concentration in blood is obtained within the first 15 min, followed by liver and brain, 30 and 60 min after i.p. administration, respectively. The memantine concentration per g brain tissue is almost identical to the dose applied per g body weight. Lower brain levels are obtained if memantine is given p.o. However, in contrast to the rapid decrease after i.p. treatment, the plateau reached 1 h after p.o. administration does not level off within the next 3 h.

Amantadine↗
PubMed1980

Distribution of metabolism of the potential anti-parkinson drug memantine in the human.

GC-MS of tissue extracts obtained from a parkinsonian patient who died from secondary causes during memantine (1-amino-3.5-dimethyladamantane) treatment showed the drug to be largely unmetabolized. In the kidney and liver a second peak corresponded to less than 1% of the main temporal lobe, hypothalamus and pons. Thus treatment with 2 X 10 mg memantine/day reveals an accumulation of the drug in microM concentrations in the brain. This is probably high enough to explain the ameliorating effect of memantine treatment in patients suffering from Parkinson's disease.

Aged↗
PubMed1980

[Increased hair growth during prolonged tocolytic therapy with fenoterol. Measurements of testosterone, androstandiol, cortisol and ACTH (author's transl)].

28 patients out of 30 treated with a longtime i.v. infusion of Fenoterol showed an intensive hair growth all-over the skin not especially showing the Linea alba and the beard. To investigate the normal hair growth during pregnancy we examined 384 volontiers just before delivery. 2,8% showed an android hair growth and 3,6% a more intensive hair growth all over the skin. To investigate the role of hormones in the excessive hair growth following i.v. tocolysis with Fenoterol, we measured Testosterone, 5 a-androstan-3 beta-17 beta-diol, Cortisol and ACTH. In contrast to the reports in literature we found a decrease in Testosterone during the treatment with Fenoterol. After an initial fall Androstandiol showed a slight rise in the 3rd week of therapy. ACTH and Cortisol remained unchanged. Contrary to the literature we did not see any augmentation of plasma Cortisol. We conclude, that androgen metabolites are not the reason for the intensive hair growth. The hair growth may be due to a more intensive metabolism within the hair follicle.

Adolescent↗