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Biomedical subjects

G Sullivan

Publications and source records attributed to G Sullivan.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Factors affecting virus plaque confirmation procedures.

Direct plaque counts obtained by using the monolayer cell culture assay technique reliably confirmed the number of viruses isolated. Analysis revealed some significant differences in the false-positive rate, depending on the test method used or virus samples evaluated. Plaques from laboratory stock viruses showed a higher confirmation rate than sewage plaque isolates. Test results with laboratory stock viruses suggested that confirmation rates may be affected by virus types present in the sample. Plaques picked by the stab and scrape method and immediately passed into cell culture tubes produced the most reliable counts as compared to those picked by the stab only method or those stored at -70 degrees C in Earle's balanced salt solution with or without fetal calf serum. Plaque confirmation using this method was 90% or better. Although the term 'false positive plaque' has been applied to a particular plaque that was not confirmed, five of ten plaques picked by the stab and scrape method in one series of experiments were confirmed when repicked by the same method from original plaque bottles, indicating that a substantial number of unconfirmed plaques may be caused by plaque transfer techniques.

Animals↗
PubMedDate not supplied

Psychiatric disorder and outcome in irritable bowel syndrome.

A link between irritable bowel syndrome (IBS) and psychiatric illness is well recognized. The authors set out to establish whether a group with a risk of poor outcome IBS could be identified at presentation to a general hospital clinic in a prospective series of 70 subjects. Potential risk factors showed no correlation with IBS outcome at 6-9 months. There was a high rate of persistent mental illness. Clinicians offering specialist care for IBS should consult with psychiatric services to provide assessment irrespective of IBS outcome if major psychopathology is not to be neglected.

Adolescent↗
PubMedDate not supplied

Migraine precipitated by head trauma in athletes.

Three members of a university football team were evaluated because of migraine symptoms precipitated by head trauma. Analysis of the clinical data from these cases, as well as eight previously reported in athletes, reveals that the head trauma is usually minor and not associated with amnesia; and, after a symptom-free interval most often of several minutes, visual, motor, sensory, or brainstem signs and symptoms begin. These usually last for approximately 15 to 30 min and are followed by headache frequently accompanied by nausea and vomiting. In 9 of 11 cases, attacks have occurred with subsequent head trauma. Only 4 of the 11 athletes admitted to spontaneous episodes, however, the incidence may be higher since they have not been followed by a sufficient period of time. Prophylaxis with antimigrainous drugs does not appear to be indicated. The decision as to future participation in contact sports is based primarily on the results of a thorough neurologic evaluation. The possible long-term sequela of this apparent "benign" condition, particularly in those athletes with repeated episodes, is not known since the entity has only recently been recognized. A migraine attack occurring in the course of an athletic event, particularly contact sports, can simulate a serious neurologic emergency. Despite its frequency in the general population and a propensity for onset in the first three decades of life, migraine has not been appreciated in the past as a possible significant sports medicine problem.

Adolescent↗