PubMed Health⌕ Search

Biomedical subjects

G Talukder

Publications and source records attributed to G Talukder.

At least 55 records · Page 3Linked to original sources
PubMed1992

The blood group and haemoglobin types of the Santals.

A total of 488 blood samples from tribal as well as non-tribal donors of Bihar and West Bengal were analysed for presence of abnormal haemoglobin variants. Out of 488 samples ABO and Rh blood groups were studied in 468 samples. Predominance of group B was noted in both the tribal and non-tribal groups and absence of Rh(d) was noted in non-tribal population with the exception of the tribals where 4.42% Rh(d) gene was found. Incidence of haemoglobin E gene was very low in most cases but relatively higher in non-tribal populations, while the range of raised haemoglobin A2 varied from 5.84% to 8.80%.

ABO Blood-Group System↗
PubMed1992

Cytotoxic effects of Sumicidin, a type II synthetic pyrethroid, on mice in vivo at 6, 12 and 24 h after exposure.

The ability of Sumicidin, a synthetic pyrethroid, to induce cytotoxicity in Swiss albino mice was evaluated before completion of the first cell division following oral exposure. Mice were administered orally different concentrations (32.50, 75 and 150 mg/kg body wt) of Sumicidin in a single dose. Mitotic index and chromosomal aberrations were screened after 6, 12 and 24 h following exposure. The frequency of dividing cells decreased significantly, indicating mitostatic action. The frequency of chromosomal aberrations was directly related to the concentrations used and the duration after exposure, and was significant at all concentrations. The highest frequency of aberrations was observed 24 h after exposure.

Animals↗
PubMed1991

Modifying role of Phyllanthus emblica and ascorbic acid against nickel clastogenicity in mice.

Nickel, a major environmental pollutant is known for its clastogenic and carcinogenic potential. Dietary inhibitors of mutagenesis and carcinogenesis are of particular importance since they may have a role in cancer prevention. In the present investigation, aqueous extract of edible dried fruits of Phyllanthus emblica, a well known medicinal plant, was fed to Mus musculus for seven consecutive days prior to treatment with different doses of nickel chloride (10, 20 and 40 mg/kg body wt.); the fruit extract significantly reduced the frequency of CA/cell, the percentage of aberrant cells and the frequency of micronuclei induced by all doses of nickel in the bone marrow cells of mice. Ascorbic acid, a major constituent of the fruit, fed for 7 consecutive days in equivalent concentration as that present in the fruit, however, could only alleviate the cytotoxic effects induced by low doses of nickel; at the higher doses it was ineffective. The greater efficacy of the fruit extract could be due to the interaction of its various natural components rather than to any single constituent. The study assumes importance in view of the widespread human exposure to nickel compounds.

Animals↗
PubMed1991

Karyotype studies of patients with gonadal tumors.

Chromosomes from the peripheral blood of 10 patients with gonadal tumors were studied to detect any inherent chromosomal defect. Aneuploidy, breakages and loss of chromosome X were observed. In all cases the karyotype was compatible with the phenotype.

Adenocarcinoma, Mucinous↗
PubMed1991

Cytogenetic damage induced in vivo to mice by single exposure to cesium chloride.

Female laboratory bred albino mice (2n = 40) were orally administered cesium chloride (CsCl) in aqueous solution as a single dose and the damage induced at the chromosomal level was observed in bone marrow cells after 6, 12, 18, and 24 hours of exposure. The concentrations of the chemical given were calculated as fractions of the LD50, namely 1/5, 1/10, and 1/20. The cytogenetic endpoints screened for were chromosomal aberrations (CA) and divisional frequency or mitotic index (MI). The frequency of chromosomal aberrations induced was directly proportional to the concentration of the chemical administered. The highest dose was the most toxic and was considered to be the maximum tolerance level. Effects on divisional frequency were variable, the highest concentration being significantly mitostatic, the middle one ineffective, and the lowest slightly mitogenic. In general, the observations indicate that CsCl is clastogenic when administered orally to mice in vivo and the effects are dose-dependent.

Analysis of Variance↗
PubMed1991

Comparative efficacy of chlorophyllin in reducing cytotoxicity of some heavy metals.

The potential of chlorophyllin in reducing clastogenicity was studied against two concentrations of each of three potent metallic clastogens (cesium chloride, mercuric chloride and cobalt chloride) in bone marrow cells of mice in vivo. The respective salts and chlorophyllin were administered orally to mice by gavaging in different combinations. Simultaneous administration of chlorophyllin with both concentrations of each salt reduced the clastogenic effects in the order Cs greater than Hg greater than Co. Chlorophyllin could not decrease the clastogenic effects when administered 2 h before the salts.

Animals↗
PubMed1991

Cytotoxicity of zinc chloride in mice in vivo.

Intraperitoneal administration of zinc chloride (ZnCl2) to Swiss albino mice in vivo induced a significant (p less than or equal to 0.05) increase in the frequencies of chromosomal aberrations of the bone-marrow cells at all concentrations used following acute (7.5, 10, 15 mg/kg body weight) and chronic (2.0, 3.0 mg/kg body wt) treatment. The degree of clastogenicity was directly proportional to the concentrations (p less than or equal to 0.05, trend test) and indirectly to the period of treatment (p less than or equal to 0.05, ANOVA test). It induced a dose-dependent, statistically significant increase (Mann-Whitney U statistics, Student's t-test) in sperm-head abnormalities. The data designate ZnCl2 as a potent clastogen and as a toxic chemical at the concentrations used.

Animals↗
PubMed1991

Modification of cesium toxicity by calcium in mammalian system.

The interaction between cesium chloride CsCl and calcium chloride CaCl2 was observed in bone marrow chromosomes of mice. The two salts were administered orally to laboratory bred Swiss albino mice in vivo singly or one followed by the other, or both simultaneously. CsCl induced chromosomal aberrations in frequencies directly proportional to the dose administered. The frequency of aberrations was reduced significantly when the two chemicals were administered simultaneously or when CaCl2 was given 2 h before CsCl. Thus, CaCl2 is able to protect against the cytotoxicity of CsCl.

Administration, Oral↗
PubMed1991

Chromosomal aberrations induced by cobaltous chloride in mice in vivo.

The effects of cobaltous chloride in inducing chromosomal aberrations were observed on laboratory bred mice in vivo after single oral administration of different fractions (1/10, 1/20, 1/40) of the lethal toxic dose of the salt. Bone marrow cells were flushed out and processed for chromosome studies following colchicine, hypotonic, giemsa, air drying procedure. The parameters screened were chromosomal aberrations, with and without gaps and break per cell. Slides were screened after the expiry of 6, 12, 18, and 24 h. Statistical analysis indicated the clastogenic effects of the salt. The degree of chromosome damage was directly related to the concentration, and also to the period after administration. The different stages of the cell cycle were affected.

Administration, Oral↗
PubMed1991

Frequency of chromosome aberrations induced by trimethyltin chloride in human peripheral blood lymphocytes in vitro: related to age of donors.

Human peripheral blood lymphocytes of healthy male and female individuals of different age groups were treated with two aqueous doses (0.5 microgram and 1.0 microgram) of trimethyltin chloride in mitogen stimulated and serum supplemented culture medium for 72 h at 37 degrees C. Chromatid and chromosome types of aberrations were observed to be increased in both treated sets in all age groups. Significant variations were observed between age groups (P less than 0.001) and between experimental sets (P less than 0.001). Moreover, interaction of chemical and donors age was statistically highly significant (P less than 0.05-P less than 0.001). However, no linear correlation between the increase of donor's age and aberrations was observed.

Adolescent↗
PubMed1991

Effect of chlorophyllin on mercuric chloride-induced clastogenicity in mice.

The effect of chlorophyllin (1.5 mg/kg body weight) on the clastogenicity of mercuric chloride (HgCl2) was studied in vivo in mouse bone marrow cells. HgCl2 (3.0, 6.0 and 12.0 mg/kg body weight) administered by gavage induced chromosomal aberrations at frequencies directly proportional to the dose. Chlorophyllin was not clastogenic, and significantly reduced the mitotic index when given alone. Chlorophyllin administered simultaneously with HgCl2 significantly reduced the frequencies of chromosomal aberrations in a dose-dependent manner. When given simultaneously with the lowest HgCl2 concentration tested (3.0 mg/kg body weight), chlorophyllin provided total protection. A lower degree of protection was given by chlorophyllin administered 2 hr before HgCl2. The data demonstrate the potential of green plant components to modify the genotoxic activity of HgCl2 when administered orally.

Administration, Oral↗
PubMed1991

Inhibition of clastogenic effects of cesium chloride in mice in vivo by chlorophyllin.

The antagonistic effect of chlorophyllin was tested in reducing the clastogenic action of cesium chloride (CsCl) in vivo on mice bone marrow cells. CsCl induced chromosomal aberration in frequencies directly proportional to the dose administered. Chlorophyllin, when given alone, was not clastogenic even at a concentration of 1.5 mg/kg body wt. of the animal. Simultaneous administration of chlorophyllin and CsCl reduced chromosomal aberrations significantly at 24 h. Exposure to the same dose of chlorophyllin 2 h before exposure to CsCl also decreased clastogenic effects but to a lesser extent. These findings are of importance in view of the uptake of radioactive Cs by green plants after nuclear fallout.

Animals↗
PubMed1991

Relationship of clastogenic effects of zirconium oxychloride to dose and duration of exposure in bone marrow cells of mice in vivo.

Zirconium oxychloride was administered as a single oral dose to laboratory-bred Swiss albino mice corresponding to 1/2, 1/6 and 1/20 of the LD50 values. Bone marrow cells were screened after 6, 12 and 24h for chromosomal aberrations following an air-drying-Giemsa schedule. The frequencies of chromosomal breaks and alterations induced increased significantly at a rate directly proportional to the concentration used. The increase was also related to the period after exposure, although to a less extent than the concentration used. No direct relationship could be observed to the sex of the animal.

Administration, Oral↗
PubMed1991

Cytotoxic effects of cobalt chloride on mouse bone marrow cells in vivo.

Various dilutions (1/10, 1/20, 1/40) of the lethal toxic dose of cobalt chloride, a non-carcinogenic salt, were found to be clastogenic to bone marrow cells of mice when administered orally in vivo. The clastogenic effects, mainly chromosome breaks, increased significantly with increasing concentration. The frequency of cell division was affected only by higher concentrations of the salt.

Administration, Oral↗
PubMed1991

Effects of aluminium salts on bone marrow chromosomes in rats in vivo.

Oral administration of aluminium sulphate to laboratory bred Rattus norvegicus for prolonged period induced dose dependent inhibition of dividing cells and an increase in chromosomal aberrations. The effect was not influenced by the duration of exposure. The toxicity of the two salts, aluminium sulphate and potassium aluminium sulphate, did not differ significantly at doses in which the metal contents were kept constant.

Alum Compounds↗
PubMed1991

Effects of culture media on spontaneous incidence of mitotic index, chromosomal aberrations, micronucleus counts, sister chromatid exchanges and cell cycle kinetics in peripheral blood lymphocytes of male and female donors.

The spontaneous incidence of mitotic index (MI), chromosomal aberrations (CA), micronucleus counts (MNC), sister chromatid exchanges (SCE) and cell cycle kinetics (CCK) were studied in human peripheral blood lymphocytes grown in M199 and RPMI-1640 culture media. Lower frequencies of CAs, MNC and SCEs were observed in lymphocytes cultured in medium RPMI-1640. The reduction of the MI and the replicative index in M199 medium showed delayed cell cycle kinetics.

Adolescent↗
PubMed1990

Clastogenic activity of strontium chloride on bone marrow cells in vivo.

Oral administration of different concentrations of Strontium chloride to laboratory bred mice in vivo induced chromosomal aberrations in bone marrow cell metaphase preparations. The degree of clastogenicity was directly proportional to concentration used at 6, 12, and 24 h of treatment. Duration of treatment could only be related positively in the lower doses. The females showed greater susceptibility than the males at all concentrations used.

Animals↗