Biomedical subjects

G Troncone

Publications and source records attributed to G Troncone.

41 records · Page 3Linked to original sources

PubMedDate not supplied

Apoptosis and cytologic differentiation in hepatocellular carcinoma on fine needle aspiration samples.

OBJECTIVE: To evaluate possible alterations of the mechanisms leading to apoptosis in hepatocellular carcinoma by studying bcl-2 expression on fine needle aspiration biopsy (FNAB) samples using immunocytochemistry. STUDY DESIGN: The study was performed on a series of 84 hepatocarcinomas aspirated under ultrasound guidance. A Papanicolaou-stained smear for each case was destained and restained for bcl-2 by using the immunoperoxidase technique. bcl-2 Expression was then correlated with cytologic grading and the size of the tumor. RESULTS: In 16 cases (19%), bcl-2 immunostaining gave a specific cytoplasmic signal. Fourteen of these positive cases were well differentiated, and two were pleomorphic tumors. Six positive cases were smaller than 5 cm, 3 were larger than 5 cm, and 7 were diffuse. CONCLUSION: A significant percentage of hepatocellular carcinomas produce and accumulate bcl-2 protein in cell cytoplasm. bcl-2 Expression can be detected on destained cytologic smears. bcl-2 Expression seems to correlate with the cytologic degree of differentiation but not with the size of the tumor.

Apoptosis↗

PubMedDate not supplied

[Leydig-cell tumors of the testicle].

Leydig's cells tumor account for 1-2% of all testicular tumors. The authors report a rare case of benign Leydig's cells tumor in a young man with gynecomastia. They examine the embryogenesis, the hormonal modifications, the criteria of malignancy, the treatment and results. They present a review of the literature pertaining to this rare tumors.

Adolescent↗

PubMedDate not supplied

Fine needle aspiration biopsy diagnosis of giant-cell tumor of bone. An experience with nine cases.

The cytologic findings in nine cases of giant-cell tumor (osteoclastoma) of bone diagnosed by fine needle aspiration (FNA) biopsy are described. The aspirates contained a dual population of cells, consisting of mononucleated tumor cells and multinucleated giant cells (osteoclasts). The tumor cells were usually evident in the FNA smears as cohesive perivascular clusters; some were present as sheets or single cells. The most striking feature, which may best suggest the diagnosis of giant-cell tumor in an FNA smear, was the attachment of the osteoclasts to the cohesive groups of tumor cells. Even when the cells were more dissociated, there was a rather regular distribution of the two types of cells. This series further demonstrates that FNA biopsy permits an accurate cytopathologic diagnosis of giant-cell tumor and that its use can clarify the clinical and radiologic impressions of these bony lesions preoperatively. The differential diagnosis between osteoclastoma and other giant-cell containing bone lesions is discussed in detail.

Adolescent↗

PubMedDate not supplied

Cytologic observations preceding high grade squamous intraepithelial lesions.

There is some evidence that a significant proportion (12%) of women, prospectively followed after negative cervical cytologic findings, develop tissue-proven high grade squamous intraepithelial neoplasia (HSIL) within a short period (up to 24 months). The present study was undertaken to address the validity of this statement since such a high prevalence of spontaneous HSIL, if true, may challenge the value of an annual cervicovaginal cytologic smear examination. Cytologic smears collected from 82 patients with tissue-proven HSIL and obtained in the preceding 24 months were evaluated. The vast majority (90%) of these smears revealed evidence of cervical epithelial abnormalities prior to the development of HSIL-88% at 24 months and 96% at 12 months. The cytomorphologic features of low grade squamous intraepithelial neoplasia were observed in 45% of the smears, while the remaining cases were not normal or negative but had cytologic evidence of human papillomavirus (HPV)-associated changes (described elsewhere), including binucleation and multinucleation, epithelial plaques and pearl formations, and hyperkeratosis, dyskeratosis and parakeratosis. Persistent negative cytology was found in only three cases. Although these HPV-related cellular changes may not have an independent predictive value for HSIL, our study demonstrated that their awareness and detection can help to identify women at risk of potentially more advanced lesions. Thus, an annual cytologic smear examination is valuable and accurately reflects the presence of HPV-associated epithelial changes and precursor lesions of HSIL.

Adolescent↗

PubMedDate not supplied

Fine needle aspiration biopsy of the spleen. A useful procedure in the diagnosis of splenomegaly.

This study analyzed a series of 140 consecutive fine needle aspiration biopsies of the spleen (sFNAB) collected over a period of nine years. sFNABs were performed by the authors using a 22-gauge spinal needle, and 23-gauge needle in pediatric patients, with a subdiaphragmatic approach. Ultrasound assistance was utilized in 35 cases. Four of the cytocentrifuge specimens were also prepared with part of the aspirated material and used for immunocytochemical staining. Study of these biopsy specimens revealed various benign conditions, such as white pulp hyperplasia, myeloid metaplasia, storage disease, and granulomatous processes and abscesses. Malignant neoplasms were represented by non-Hodgkin's and Hodgkin's lymphomas, leukemias, malignant histiocytoses and metastases. sFNAB was the only diagnostic procedure employed for the morphologic assessment of 60 benign and of 2 malignant cases. In all the other cases sFNAB gave the first diagnosis that was confirmed on surgical specimens. In 2 cases splenic bleeding occurred; one of them required splenectomy. Diagnostic and technical problems are discussed. The authors consider sFNAB a useful procedure, especially in the study of hematologic disorders and in all cases in which splenomegaly is not related or correlated with any clinical finding.

Adolescent↗