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Biomedical subjects

G Turowski

Publications and source records attributed to G Turowski.

At least 37 records · Page 2Linked to original sources
PubMed1996

[Immunology of early pregnancy. I. Immunologically competent cells in the endometrium and decidua].

Changes in the proportion of macrophages, granulocytes and subpopulations of lymphocytes in the secretory endometrium and decidua have been reviewed. The decidual transformation and principles of the neutralization of an adaptative response to embryonic tissues as well as the fetal growth promoting decidual factors have been discussed. The immunological response can broadly be divided into two types, cell mediated and humoral. Cell-mediated immune responses involve the activation of macrophages and the induction of macrophages and the induction of cytotoxic CD8+ and CD4+. T cells, whereas humoral immunity is characterized by antibody production. These two arma of the immune response are regulated by distinct subsets of CD4+ helper T cells, termed Th1 and Th2 cells, which secrete different patterns of cytokines. The pregnancy induced possible Th2 prevalence so as role of such factors like PGE2, TGF2, 1.25-(OH)2-Vit. D3, IL-2, progesteron, estrogens and dehydroepiandrosteron have been reviewed.

Antibody Formation↗
PubMed1996

[Immunology of early pregnancy. II. Decidua and trophoblast relationship].

Immunological competent cells and their cytokines exert a potent influence on both the placental function and the fetus. The different fetal and placental cells and in the same way modify cytokines production by the immunological system. The further characteristic of decidual lymphocytes subpopulations and their role in the regulation of embryonic development has been presented. The interactions between cytokines and growth factors derived from both decidua and trophoblast have been reviewed. The role of HLA-G, TLX and expressed cell differentiation antigens (complement system suppression) in the early pregnancy protection as well as trophoblast invasion inhibition have been discussed. The potential role of these factors in the early pregnancy loss has been considered.

Antigens, Differentiation↗
PubMed1995

[Class I HLA antigens in children from families with congenital adrenal hyperplasia].

Congenital adrenal hyperplasia (CAH) is a syndrome of adrenal steroid metabolism errors with an autosomal inheritance model. The most common metabolic defect is 21-hydroxylase deficiency. It has been demonstrated that 21-hydroxylase genes are in close association with HLA antigens. I HLA antigens were typed in a group of 32 families of children with CAH-type 21-hydroxylase deficiency with salt loss. The antigen frequencies were determined and compared to those of the control population. The studies revealed that two HLA antigens determined by the B Locus, i.e. HLA-B47 and HLA-B61, showed a highly significant frequency (chi 2 corresponding to 404,5259 and 23,7808, respectively). The calculated relative risk and etiologic fraction values were extremely high, distinguishing the population of patients and their parents. The RR value among patients was 427.1 for HLA-B47 and 7.8 for HLA-B61 antigen. Studies on the correlation between HLA and CAH indicate an association with HLA-B47 and HLA-B61 antigens.

Adrenal Hyperplasia, Congenital↗
PubMed1995

[HLA haplotypes in families of children with congenital adrenal hyperplasia].

The haplotypes of class I HLA system were determined in a group of 32 children with congenital adrenal hyperplasia and classic form of 21-hydroxylase deficiency. The haplotype frequencies were analyzed and compared to those of the control population. Haplotypes containing the HLA-B-47 antigen were significantly more frequent among the families of patients than among the control population. Among the patients, haplotypes HLA A3-B47-Cw6 and HLA A3-B47-Cwx constituted 50% of all the observed haplotypes with the HLA-B47 antigen. It is stressed that the frequency of homozygotic systems for antigens determined by locus HLA-A (33.34%) and for antigens with locus HLA-B (22.23) were much higher in the affected children.

Adrenal Hyperplasia, Congenital↗
PubMed1994

Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation.

Mucosal healing requires enterocyte migration (restitution) supplemented by proliferation. Proliferation and migration may be studied independently by thymidine uptake and proliferation-blocked cell migration using human Caco-2 enterocyte monolayers in culture. Since epidermal growth factor (EGF) promotes mucosal healing and the EGF receptor is a tyrosine kinase, we hypothesized that tyrosine kinases might therefore modulate enterocyte migration and proliferation. The tyrosine kinase inhibitors genistein and 2,5-dihydroxymethylcinnamate, which block kinase ATP-binding and substrate-binding sites, respectively, were studied alone and with EGF. Proliferation was blocked with mitomycin. Although each inhibitor decreased basal and EGF-stimulated monolayer expansion when cell proliferation occurred, neither genistein nor 2,5-dihydroxymethylcinnamate decreased migration when proliferation was blocked. However, each inhibitor prevented EGF stimulation of proliferation-blocked migration and thymidine uptake. More substantial inhibition of basal proliferation by genistein correlated with increased protein-linked DNA breaks, which may reflect nonspecific inhibition of DNA topoisomerase activity by genistein. The more specific 2,5-dihydroxymethylcinnamate blocked changes in the alpha 2 integrin subunit organization which may modulate EGF-stimulated migration. Antiproliferative effects of tyrosine kinase inhibitors decrease basal monolayer expansion but true basal enterocyte migration appears independent of tyrosine kinase regulation. However, a specific tyrosine kinase-dependent modulation of cell-matrix interaction inhibits EGF-stimulated migration.

Cell Division↗
PubMed1985

The maximum likelihood estimation of haplotype frequencies of HLA and HLA-like systems and addition loci of genes with the different modes of inheritance.

Conventional formula for the estimation of haplotype frequencies proposed by Yasuda was extended for the haplotypes constituting three HLA loci and two additional loci of genes with codominant alleles. The computer programme FREHAP estimates 32 haplotype frequencies rijkmn by the method of maximum likelihood, as well as it calculates the marginal frequencies rijk.. and r...mn, the matrix of expected numbers and the value of chi-square for the goodness of fit. The FREHAP has been programmed in FORTRAN IV for a CDC CYBER 72 computer.

Gene Frequency↗
PubMed1984

Central nervous system malformations in the Kraków region. II. Case control studies on the human leukocyte antigen genotype and haplotype frequencies.

A case control study of human leukocyte antigen haplotypes was performed on a sample of 44 index families of children with central nervous system (CNS) malformation and 36 families of healthy newborn infants. HLA typing for antigens determined by loci A, B, and C was done on parents and offspring. The HLA genotypes were inferred from segregation in families and the HLA gene and haplotype frequencies were obtained by direct gene counting. Case-control comparison did not uncover significant differences at any HLA allele. The haploytype pattern in the group of index cases did not show association with CNS malformations. The HLA phenotype compatibility among the probands' and controls' parents was within the same range except for locus C where parents of children with neural tube defects (NTD) less frequently shared common HLA-C antigens. The segregation of paternal haploytypes showed significant deviation in the families of NTD infants.

Adult↗
PubMed1984

The effect of penicillin on skin graft survival in mice.

The effect of penicillin administered to mice graft recipients on the allograft survival time as well as modulation of the rejection were studied. The skin graft donors (DBA mice strain) and recipients (CBA mice strain) were incompatible in the major histocompatibility system H-2. The control group was made up of the mice which had received skin grafts only. The penicillin was injected intraperitoneally 5 times in daily doses of 10,20, and 30 thousand units per mouse from the 6th to 2nd day before, or in doses of 10, 15, 20, 30 and 40 thousand units on days 0 to 4 after skin grafting. One experimental group of CBA mice had been treated with a single dose of 30,000 units of penicillin only, just before skin grafting. The treatment of mice with penicillin in doses of 15, 20 and 30 thousand units after skin grafting showed an immunostimulating influence on the immunological response of the graft host, manifested as statistically highly significant (p less than 0.001), shortening of graft survival, the onset and duration of time of graft rejection. The antibiotic administered to mice once on operation day prolonged the onset of graft rejection only. No statistical difference between the whole course of skin graft rejection in the mice pre-treated with penicillin and untreated mice of the control group was stated. The results indicate modulation effect of penicillin on the cell immune response of the host, depending on the dose, multiplicity and time of its administration in relation to operative day.

Animals↗