[Fibrinolytic activity of rat blood following exposure to several atherogenic factors].
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Biomedical subjects
Publications and source records attributed to G V Andreenko.
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The level of plasminogen activator activity studied by histochemical method in the myocardial tissue of rats during experimental ischemia was decreased if compared with control animals. The maximal decrease was seen the next day after the occlusion of the coronary artery, particularly in the necrotic zone. Plasminogen activator activity level began to increase in 3 days. Histochemical data were confirmed biochemically.
The effect of purified plasmin preparation on kallikreinogen isolated from rat blood plasma was studied by chromatography on DEAE-Sephadex. Plasmin was shown to affect kallikreinogen to form an active enzyme--kallikrein. The dependence of the activation degree on the plasmin activity and incubation time was studied. Evidence was found for the absence of Hagemann factor in the reaction medium, which excludes the possibility of indirect action of plasmin on kallikreinogen through the Hagemann factor fragments formed.
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