Biomedical subjects

G V Kochetkova

Publications and source records attributed to G V Kochetkova.

At least 19 recordsLinked to original sources

PubMed1977

[Relationship between the quality of the inoculum material and carminomycin biosynthesis by a culture of Actinomadura carminata].

The effect of the inoculum mycelium quality on carminomycin biosynthesis by Actinomadura carminata was studied. The time of the organism growth on the culture medium containing cornsteep liquor continued for 6 hours without losing by the inoculum of its seeding qualities during that period. The mycelium growth in the inoculum was more intensive under conditions of moderate aeration, i.e. 0.98-2.64 mg O2H1-min. Anincrease in the aeration rate up to 18.56 mg O2/1-min resulted in the growth suppression up to 40 per cent. No correlation between the aeration rate during the inoculum growth and the culture capacity for carminomycin biosynthesis and of the content of the complex in active components the fermentation medium were observed, when a 5-10 per cent of inoculum was used.

Actinomycetales↗

PubMed1977

[Effect of the pH of the medium on the growth of a carminomycin producer and on the biosynthesis of the carminomycin complex].

The effect of the nutrient medium acidity on the growth of the carminomycin-producing organism and formation of the carminomycin complex with its aid was studied. Satisfactory development of the organism was observed when pH of the nutrient medium on the culture inoculation was 4.9 to 8.5. A shift in the alkaline and acid values of pH to 6.3--6.4 was registered during the first 24 hours of the culture growth. The biosynthesis of carminomycin depended on the culture growth time and pH value of the nutrient medium and culture fluid during the fermentation process. Maximum production of the carminomycin complex and its active components was observed on the 7th--8th day of the culture growth. The medium reaction close to the neutral one was optimal for biosynthesis of the carminomycin complex and its active components. Under acid, as well as alkaline conditions the antibiotic production was markedly lower.

Actinomycetales↗

PubMed1976

[Effect of aeration conditions on the biosynthesis of carminomycin by an actinomadura carminata culture].

The effect of the aeration rate on biosynthesis of carminomycin by Actinomadura carminata and biochemical changes in the fermentation broth on the use of 3 complex media of different composition was studied. The carminomycin-producing organism can grow and produce the antibiotic within the ranges of the aeration changes from 0.98 to 18.56 mgO2/1-min. A decrease in the maximum rate of oxygen dissolution up to 0.98 mgO2/1-min resulted in some decrease in the activity level. Intensive aeration, i.e. 18.56 mgO2/1-min induced suppression of the antibiotic production by 25 per cent.

Aerobiosis↗

PubMed1976

[Madumycin biosynthesis by an Actinomadure flava culture under conditions of varying aeration].

Production of madumycin I and madumycin II by 2 strains of Actinomadure flava 2171 was studied. Intensive aeration was an obligatory condition for the antibiotic biosynthesis. The content of madumycins I and II in the culture fluid depended on the strain, aeration conditions and fermentation cycle. Strain 2171/852 produced maximum amounts of madumycin I and madumycin II by the 2nd--3rd and 5th days of cultivation respectively. Strain 2171/2544 mainly produced madumycin I during the whole period of fermentation.

Aerobiosis↗

PubMed1976

[Lincomycin formation by strain R-367 on a complex medium not containing corn steep].

A new complex medium for biosynthesis of lincomycin by strain R-367 was developed using one-factor experiments and mathematical schemes of planning. A complex of mineral salts containing nitrogen, sulphur, phosphurus, magnesium and trace elements was introduced into the content of the new medium. This provided elimination of corn steep liquor from the medium. The linomycin production level in the fermentation broth with the use of the new medium was at an average 2.5 times higher than that on the initial medium containing corn steep liquor.

Culture Media↗

PubMed1975

[Optimization of the nutrient medium makeup for the biosynthesis of carminomycin using mathematical methods of experimental design].

The nutrient medium for biosynthesis of carminomycin by Actinomadura carminata, strain 4281 was optimized with the methods of mathematical planning of the experiment. The starting variant of the medium, production of carminomycin on which was assumed equal to 100 per cent was enriched during the experiments carried out according to the patterns of the orthogonal latin squares. The production levels of carminomycin on the above medium was more than 3 times higher than those on the starting medium.

Actinomycetales↗

PubMed1975

[Determination of the biological activity of heliomycin by a method of diffusion in agar].

A biological method for determination of geliomycin activity using agar diffusion is described. A nutrient medium containing Hottinger broth up to 35 mg per cent of amine nitrogen, 1.5 percent of agar-agar, tap water, pH 7.8 to 8.0 was used. Bacillus subtilis ATCC 6633 served as the test-culture. Geliomycin was dissolved in 0.1 N sodium hydroxide solution.

Agar↗

PubMed1974