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Biomedical subjects

G V Ling

Publications and source records attributed to G V Ling.

At least 55 records · Page 3Linked to original sources
PubMed1983

Canine prostatic fluid: techniques of collection, quantitative bacterial culture, and interpretation of results.

Specimens of prostatic fluid were collected by ejaculation from 104 dogs after collection of a specimen of urethral contents and after collection of a urine specimen by antepubic cystocentesis. For purposes of data analysis, results of bacterial culture of the specimens were grouped according to individual colony counts in each specimen group (ie, urine, urethra, prostatic fluid). For purposes of this study, prostatic infection was defined as greater than or equal to 2 log10 of one or more bacterial species in prostatic fluid over the number of colonies of the same species in the paired urethral specimens.

Animals↗
PubMed1983

Cephalexin for oral treatment of canine urinary tract infection caused by Klebsiella pneumoniae.

Oral treatment with cephalexin resulted in bacteriologic cure in 24 of 25 (96%) dogs with urinary tract infection caused by Klebsiella pneumoniae. All 25 of the isolates of K pneumoniae were susceptible in vitro to cephalexin at a concentration of less than or equal to 64 micrograms/ml, well below the mean concentration of cephalexin attained in the urine of clinically normal dogs given similar doses of this drug.

Administration, Oral↗
PubMed1981

Results of urinalysis and bacterial culture of canine urine obtained by antepubic cystocentesis, catheterization, and the midstream voided methods.

Results of urinalysis and bacterial culture of urine specimens obtained during midstream voiding, by catheterization, and by cystocentesis were studied in 50 clinically normal dogs (25 females and 25 males). All of the specimens obtained by cystocentesis were bacteriologically sterile. Bacterial growth occurred in 26% of the catheterized specimens and in 85% of the voided specimens. The proportion of positive urine cultures from catheterized and voided specimens was higher for females than for males. Results or urinalysis did not correlate well with results of bacterial culture.

Animals↗
PubMed1981

Tetracycline for oral treatment of canine urinary tract infection caused by Pseudomonas aeruginosa.

Oral treatment with tetracycline resulted in bacteriologic cure in 23 of 25 (92%) dogs with urinary tract infection caused by Pseudomonas aeruginosa. All 25 of the isolates of Pseudomonas spp were susceptible to in vitro concentrations of tetracycline (less than or equal to 64 microgram/ml), well below the mean urine concentrations attained in the urine of clinically normal dogs given standard oral doses of tetracycline.

Administration, Oral↗
PubMed1981

Urinary tract infection resulting from catheterization in healthy adult dogs.

The urinary bladders of 70 clinically normal adult dogs of both sexes and a variety of breeds were catheterized after a urine specimen was taken from each dog by antepubic cystocentesis. All of the specimens were negative for bacterial growth after 72 hours' incubation at 37 C. Three days later, a 2nd urine specimen was taken from each dog by cystocentesis. All 35 specimens collected from male dogs at this time were culture-negative, whereas 7 of 35 (20%) specimens collected from female dogs were culture-positive. It was concluded that urinary bladder catheterization in female dogs is associated with substantial risk and should be used only when alternate methods of urine collection are not available.

Animals↗
PubMed1981

Pharmacokinetics of trimethoprim and sulfadiazine in the dog: urine concentrations after oral administration.

Serial blood samples were obtained from 12 healthy adult dogs given equivalent subcutaneous and oral doses of the antibacterial combination, trimethoprim-sulfadiazine (1:5). By using a 1-compartment open model, pharmacokinetic parameters for both drugs were estimated from the mean serum concentration data after oral administration. Trimethoprim and sulfadiazine were rapidly absorbed, reaching maximum concentrations in 1 and 4 hours with serum elimination half-lives of 2.5 and 9.9 hours, respectively. After a single oral dose (30 mg/kg, combined ingredients) was given, both drugs were present in urine for up to 24 hours at concentrations exceeding the minimum inhibitory concentrations for common pathogenic bacteria.

Administration, Oral↗
PubMed1981

Urine concentrations of gentamicin, tobramycin, amikacin, and kanamycin after subcutaneous administration to healthy adult dogs.

Gentamicin, tobramycin, amikacin, and kanamycin were given subcutaneously in separate trials to healthy adult dogs of both sexes. Daily dosage levels were as follows: gentamicin, 6.6 mg/kg of body weight; tobramycin, 3 mg/kg; amikacin, 15 mg/kg; and kanamycin, 11 mg/kg. Gentamicin, tobramycin, and amikacin were given in divided doses of 8-hour intervals for 5 consecutive 8-hour periods, whereas kanamycin was given in divided doses at 12-hour intervals for 4 consecutive 12-hour periods. Mean 8-hour urine concentrations +/- 1 SD were gentamicin, 107 +/- 33 microgram/ml, tobramycin, 66 +/- 39 microgram/ml; and amikacin, 342 +/- 153 microgram/ml. Mean urine concentrations (+/- 1 SD) for kanamycin were 473 +/- 306 microgram/ml in urine collected between 0 and 6 hours after dosing and 63 +/- 47 microgram/ml in urine collected 6 to 12 hours after dosing.

Amikacin↗
PubMed1980

Incidence of R-plasmids in fecal flora of healthy household dogs.

Rectal swabs were taken from healthy household dogs that, insofar as could be determined, had not received antimicrobial drugs. Tetracycline-resistant coliforms comprised 80 to 100% of the total number of coliforms in 61 (65%) of the 94 dogs sampled. The median number of other resistance determinants possessed by these tetracycline-resistant coliforms was 5.1. Of the tetracycline-resistant strains studied, 97% were resistant to streptomycin; 76% were resistant to sulfonamides; 59% were resistant to ampicillin; 59% were resistant to kanamycin/neomycin; and 40% were resistant to chloramphenicol. A total of 64% of the strains was shown to transfer resistance by conjugation or by the aid of the sex factor F. Of the strains transferring resistance, 33% were found to transfer all of their resistance determinants.

Animals↗
PubMed1980

Urine concentrations of five penicillins following oral administration to normal adult dogs.

Penicillin G, penicillin V, ampicillin, hetacillin, and amoxicillin were administered separately per os to clinically normal adult dogs of both sexes at 8-hour intervals for five consecutive 8-hour test periods. All urine was collected from each dog during each test period and was assayed for antimicrobial activity. Daily doses of the antimicrobics used were as follows: penicillin G, 110,000 U/kg of body weight; penicillin V, 77 mg/kg; ampicillin, 77 mg/kg; hetacillin, 77 mg/kg; and amoxicillin, 33 mg/kg. Mean 8-hour urine concentrations (+/- 1 SD) were as follows: penicillin G, 294 +/- 210 U/ml; penicillin V, 148 +/- 98 micrograms/ml; ampicillin, 309 +/- 55 micrograms/ml; hetacillin, 300 +/- 156 micrograms/ml, and amoxicillin 201 +/- 93 micrograms/ml. Among the three antimicrobials administered at the same daily dosage, ie, penicillin V, ampicillin, and hetacillin, the mean urine concentrations of penicillin V were significantly lower (P < 0.01) than were concentrations of ampicillin and hetacillin. The mean urine concentrations of the latter two did not differ significantly (P > 0.05).

Administration, Oral↗
PubMed1980

Relation of antibody-coated urine bacteria to the site(s) of infection in experimental dogs.

Antibody-coated bacteria tests, using fluorescein-conjugated rabbit anti-canine immunoglobulin (Ig)G and anti-IgA, were conducted on 77 specimens of bladder urine obtained by antepubic cystocentesis from 25 dogs with urinary tract infections. Urine specimens from 3 dogs infected with Staphylococcus aureus were tested a total of 27 times, specimens from 10 dogs infected with Escherichia coli were tested 25 times, urine from 6 dogs infected with Proteus mirabilis was sampled and tested 18 times, urine from 3 dogs with Klebsiella pneumoniae was sampled and tested 4 times, and specimens from 3 dogs infected with P rettgeri, Enterobacter cloacae, or Streptococcus faecalis were tested once each. Specimens of urine from the renal pelves of each dogs were obtained by percutaneous nephropyelocentesis at the same time as the specimens of bladder urine. The results of bacterial cultures of these specimens were compared with the results of the corresponding antibody-coated bacteria tests. False-positive results (ie, evidence of antibody coating of bladder urine bacteria but no bacterial growth from corresponding renal pelvis urine specimens) were obtained in 13 of 22 (59%) tests of dogs infected with S aureus, 12 of 13 (92%) tests in dogs infected with P mirabiis, 5 of 18(28%) tests in dogs infected with E coli, and 1 of 4 (25%) tests in dogs infected with K pneumoniae. False-negative results (ie, bacterial growth from renal pelvis urine specimens but no evidence of antibody coating of bacteria in the corresponding specimen of bladder urine) occurred in 5 of 7 (71%) specimens infected with E coli, in 4 of 5 (80%) specimens infected with P mirabilis, and in 5 of 5 (100%) specimens infected with S aureus. It was concluded that the antibody-coated bacteria test does not provide accurate information to support the diagnosis of pyelonephritis in the dog.

Animals↗
PubMed1980

Urine concentrations of chloramphenicol, tetracycline, and sulfisoxazole after oral administration to healthy adult dogs.

Chloramphenicol, tetracycline, and sulfisoxazole were administered (orally) in separate trials to clinically healthy adult dogs of both sexes at 8-hour intervals for five consecutive 8-hour test periods. All urine was collected from each dog during each test period and an aliquot from each period was assayed for antimicrobial activity. Daily doses of the antimicrobics were as follows: chloramphenicol 99 mg/kg of body weight, tetracycline 55 mg/kg, and sulfisoxazole 66 mg/kg. Mean 8-hour urine concentrations (+/- 1SD) for chloramphenicol were 124 +/- 40 micrograms/ml; for tetracycline, 138 +/- 65 micrograms/ml; and for sulfisoxazole, 1,466 +/- 832 micrograms/ml. The mean 8-hour percentages of the doses of drug eliminated in active form in the urine were 6.3 +/- 2.6% for chloramphenicol, 11.2 +/- 2.0% for tetracycline, and 68.5 +/- 2.1% for sulfisoxazole.

Administration, Oral↗
PubMed1979

Gentamicin for treatment of resistant urinary tract infections in dogs.

Gentamicin was administered parenterally for 6 days to 43 dogs with urinary tract infections. The daily dosage of 6.6 mg/kg (3 mg/lb) was divided into equal parts and given IM or SC at 8-hour intervals. Dogs selected for treatment with gentamicin had urinary infections that had not responded to treatment with other antimicrobial agents or had bacterial isolates from urine that were resistant to several antimicrobial agents on in vitro susceptibility tests. Response to treatment, defined as negative urine culture on the last day of therapy or 4 to 14 days after completion of the therapeutic course, included 20 of 22 (91%) infections caused by Escherichia coli, 8 of 9 (89%) infections caused by Kebsiella pneumoniae, 6 of 7 (86%) infections caused by Proteus spp, and 6 of 7 infections caused by Pseudomonas spp. These four species comprised 84% of the bacteria isolated from the dogs in this study.

Animals↗
PubMed1979

Canine hyperadrenocorticism: pretreatment clinical and laboratory evaluation of 117 cases.

The physical findings, clinical signs, age, breed and sex distributions, and laboratory data of 117 dogs with untreated Cushing's syndrome were reviewed. Poodles, Dachshunds, and Boxers of all ages were found to be at increased risk, as were dogs of all breeds greater than or equal to 6 years old. Polydipsia, polyuria, progressive bilaterally symmetric alopecia, and abdominal distention were the most frequently observed clinical signs and physical findings. Lymphopenia, eosinopenia, above normal values of serum alkaline phosphatase, serum cholesterol, and sulfobromphthalein dye retention, and below normal urine specific gravity were the most frequent abnormalities found in the laboratory data. About 50% of the dogs had urinary tract infections. Final diagnosis was established on the basis of abnormally high plasma corticosteroid values in response to an intramuscular injection of adrenocorticotropic hormone.

17-Ketosteroids↗
PubMed1979

Trimethoprim in combination with a sulfonamide for oral treatment of canine urinary tract infections.

Trimethoprim, in combination with sulfadiazine or sulfamethoxazole was administered orally for 7 to 14 days to 84 dogs with urinary tract infections (UTI). The daily dosage of 26.4 mg/kg (12 mg/lb) was divided into 2 equal parts and administered at about 12-hour intervals. Response to treatment, based on negative urine culture during or after therapy, was 37 of 45 (82%) for UTI caused by Escherichia coli, 11 of 15 (73%) UTI caused by Proteus mirabilis, 8 of 12 (67%) UTI caused by Klebsiella pneumoniae, 6 of 6 (100%) UTI caused by Staphylococcus aureus, and 5 of 9 (56%) UTI caused by Streptococcus spp. These 5 species comprised 88% of the bacteria isolated from the dogs in this study.

Administration, Oral↗
PubMed1979

Influence of environment on adrenal cortical response to ACTH stimulation in clinically normal dogs.

Effect of testing environment on adrenal cortical responses to an injection of ACTH in clinically normal dogs was examined in three locations, presumably of increasing order of stress elicitation: in a home; veterinary hospital (VH), 4 hours in a cage; and VH, overnight in a cage. Basal cortisol (hydrocortisone) values for plasma were significantly lower (P less than 0.001) for the home group (1.8 microgram/dl) when compared with values for the VH, 4-hour cage (3.8 microgram/dl) or the VH, overnight cage (3.9 microgram/dl) groups. However, significant differences (P greater than 0.05) were not observed 2 hours after ACTH admininstration for the home group (13.7 microgram/dl); VH, 4-hour cage group (14.8 microgram/dl); or VH, overnight cage group (16.0 microgram/dl). Responses of individual dogs were consistent (P less than 0.005). The testing environment did not markedly affect results of adrenal cortical function tests for dogs when ACTH stimulation was utilized. The response of dogs to ACTH, as monitored by immunologic assay techniques (competitive protein-binding assay or radioimmunoassay), was consistent and was useful as a diagnostic aid for adrenal malfunction.

Adrenal Cortex↗
PubMed1979

Percutaneous nephropyelocentesis and nephropyelostomy in the dog: a description of the technique.

Percutaneous nephropyelocentesis or nephropyelostomy was done on 20 anesthetized dogs of both sexes with the aid of fluoroscopy. The upper urinary tracts were visualized by the use of IV radiopaque contrast material and distended by application of an abdominal compression band positioned over the caudal abdomen. Renal pelvic urine was readily obtained by both procedures in each dog. Bloody urine was an infrequent result, and usually occured only after repeated attempts had been made to enter the renal pelvic lumen. To ascertain the degree of renal parenchymal damage caused by passage of the needle and catheter, dogs were euthanatized and necropsied on the 1st, 3rd, 7th, and 14th days after the procedure was done. Repair of the tissue damage was rapid and the lesions were difficult to visualize grossly by the 14th day.

Animals↗