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Biomedical subjects

G V Samsonov

Publications and source records attributed to G V Samsonov.

At least 19 recordsLinked to original sources
PubMed1990

[Modification of hemoglobin by glutaric aldehyde and electrochemical properties of the conjugates].

Electrochemical properties of macromolecules of modified haemoglobin obtained by polycondensation with glutaric aldehyde have been investigated by means of potentiometric titration, PAG-electrophoresis, ion-exchange chromatography, and (for evaluation of isoelectric point) distribution between two aqueous polymeric phases. Introduction of additional functional groups into the macromolecule is possible by using various agents blocking polycondensation, which makes it possible to change the resulting charge and the isoelectric point.

Aldehydes↗
PubMed1981

[Oxygen transport in rats following total blood replacement with solutions of high-molecular modified hemoglobin].

In acute experiments on Wistar rats, after replacement of 92-94% of the blood with solution of high-molecular modified hemoglobin (HMH), 10.1-11.2 g%, the latter's oxygen transport function was studied. Prior to and after blood replacement, the gaseous composition of the blood and alveolar air, acid-alkaline condition of the blood, curves of hemoglobin dissociation, hematological parameters and survival of animals, were studied. The HMH was shown to preserve the ability to uptake and release oxygen, after administration into the vascular bed. The HMH solutions transporting the oxygen, sustain the life in rats during 9.7 +/- 2.3 hrs at a lethal degree of hemodilution (hematocrit 3.9 +/- 0.7%). Nevertheless, owing to high affinity between the HMH and oxygen and to low oxygen capacity of the solutions, the HMH is unable to maintain the initial conditions for oxygen transport to tissues.

Acid-Base Equilibrium↗
PubMed1975

Some properties of the protease from Aspergillus terricola.

The protease from Aspergillus terricola (terrilytin) was isolated and purified by the ion exchange method and by gel chromatography. The data on its molecular weight, isoelectric point, N-terminal amino acids were described. The protease included polysaccharide which stabilized the enzyme activity under the storage and lyophylization. Polysaccharides isolated from different fungi and yeasts were found to stabilize and activate terrilytin rising the affinity of enzyme to substrate. Terrilytin was revealed to exhibit the thrombolytic effect and expressive affinity to the fibrin in comparison with other substrates of high and low molecular weight. The enzyme showed the antigenic activity. From immune rabbit sera 7S gamma-globulins inhibiting proteolysis of caseine and 19S gamma-globulins activating the proteolysis of fibrin and fibrinogen by terrilytin were isolated by gel chromatography.

Amylases↗