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G V Selivanova

Publications and source records attributed to G V Selivanova.

49 records · Page 3Linked to original sources
PubMed1976

[A cytophotometric study of granules forming in frog erythrocytes during incubation in solutions of neutral red and novocaine].

Granules arising in the cytoplasm of Rana temporaria erythrocytes incubated in solutions containing either a stain (neutral red) or an alcaloid (novocaini) were studied cytophotometrically. The stuff concentrations in the granules were estimated under various conditions of incubations. The stuff concentrations in the granules appeared to be dozens- or hundreds--fold higher than in the incubation solutions. The average concentration in the granules did not depend either on the initial concentration of the stuff solution, or on the incubation time or on the volume of the incubation solution. The stuff concentration in the granule decreases with the increase in the granule size.

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PubMedDate not supplied

[Epidermal growth factor stimulates proliferation and DNA synthesis in ciliate cells].

We studied the effect of murine epidermal growth factor on cell proliferation and DNA synthesis in macronuclei of ciliate Tetrahymena pyriformis G1. Mitogenic effect of epidermal growth factor on proliferation-induced tetrahymena cells has been revealed. This effect is due to the induced progression of cells at G1 and, consequently, their earlier entering DNA synthesis phase of the first cell cycle. Epidermal growth factor had no mitogenic effect on the resting cells from stationary culture (G0 phase) whose development is independent of the growth factors in the medium.

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PubMedDate not supplied

[Features of the chromatin structure of erythrocytes depending on the properties of lysine-rich histones].

A comparative analysis of chromatin from erythrocytes of frog, trout and hen has been performed in correlation with properties of the nucleosomal linker histones of H1 family. In the nucleosomes from frog erythrocytes the linker histone is represented by H1(0)-like variant with amino acid sequence highly homologous to that of the hen histone H5, however the arginine content in the proteins differs (3 mol% in the frog erythrocyte H1 and 12 mol% in the hen erythrocyte H5). On the other hand histone H5 from trout being significantly different in the primary structure from the hen histone H5 is at the same time rich in arginine (9 mol%). The nucleosomal repeat length, estimated by using agarose gel electrophoresis is 201, 213 and 213 b.p. in erythrocyte chromatin from frog, trout and hen, correspondingly. Chromatin packing density in fixed nuclei from erythrocytes of frog, trout and hen as determined using cytophotometric measurements is 0.144, 0.444 and 530 pg/mu 3, correspondingly. The data support the previously made suggestion that the increase in arginine content in nucleosomal linker proteins is connected with the increase of chromatin compaction in the nuclei and elongation of the linker in the nucleosome.

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