Internal controls for normalizing DNA arrays.
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Biomedical subjects
Publications and source records attributed to G Vegarud.
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A method for kappa-casein genotyping in bulls has been developed. By analysis of DNA polymorphisms we are able to discriminate between the kappa-casein variant A and B in the bulls. This method will be an efficient tool in selection for the most desirable kappa-casein variant.
Addition of 5% xylitol to growing cultures of Streptococcus mutans OMZ 176 reduced the amount of extractable glycerol-phosphate polymers in these cells compared to S. mutans cultures grown in medium with 5% glucose added. The glycerol-phosphate polymers were extracted from the cells by hot phenol-water-extraction, and separated by column chromatography. Lipoteichoic acid (LTA) was identified by indirect haemagglutination tests and lipid analysis. The amount of LTA extracted from the cells or present in the medium was not significantly different. It is suggested that the accumulation of intracellular xylitol-phosphate observed in a previous study causes an effect similar to glucose starvation, which is known to affect the composition of the cell wall.
The present study showed that xylitol exhibited a dose-related inhibition of the growth of Streptococcus mutans in a Brain Heart Infusion medium. It is suggested that the mechanism involved may be an effect of the translocation of glucose across the bacterial cell membrane. Sorbitol showed no similar effect but caused a delay in the reaching of the stationary phase.
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