Home health care clinical experience: responding to the health care reform agenda.
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Biomedical subjects
Publications and source records attributed to G W Morris.
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Organizing a presentation that flows smoothly, logically, and creatively requires extensive preparation. Adequate preparation time gives you an opportunity to receive constructive criticism by having your colleagues critique your speech prior to public presentation. Continuous practice before a "friendly" audience helps to build confidence. Remember, after assessing your audience, knowing when and where your presentation will be given, how your speech will be presented, and attending to the above "tips," your presentation will be a winner.
Observations made during the freezing and thawing of mouse and human oocytes and mouse embryos with the cryomicroscope suggest that physical factors as well as physicochemical factors may play a role in the development of lethal damage upon thawing. The point of contact with the approaching ice front may predispose that area to the appearance of future cytoplasmic blebbing. The ice front distorts the oocyte and this distortion remains during its subsequent thermal history and is unrelated to desiccation distortion. Ice initiates the formation of both intra- and extracellular gas bubbles which are apparent upon thawing; with the progression of the thawing process they can be seen to grow in volume. Growth of these bubbles can give rise to expanding vesicles which can totally destroy an embryo. The consequences of these physical factors for the successful cryopreservation of oocytes, embryos, tissues and organs are discussed.
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