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Biomedical subjects

G Weseloh

Publications and source records attributed to G Weseloh.

At least 55 records · Page 3Linked to original sources
PubMed1977

[Enzyme studies of synovial fluid from osteoarthritis knee joints (author's transl)].

In 40 patients who were operated on for meniscopathy, accompanied by more or less severe arthroses of the knee joints, the total activity of LDH-T and of the LDH-H isoenzyms could be evaluated. The data show that severe arthroses without any synovitic reactions as well as cases with inactive chronic synovitis showed small total activities of LDH, whereas cases with clinical and macroscopic-anatomically active synovitis (including the so-called activated arthroses) showed a great total activity of LDH. Furthermore the dominance of LDH-H isoenzyms has been found to indicate an aerobic situation of the metabolism.

Chronic Disease↗
PubMed1976

[The effects of the weather on hemophilic hemarthrosis (author's transl)].

Hemophilic hemarthroses occur more frequently in spring and autumn. In the present study, possible weather situations are investigated which are characterized by a particular action on the increasing of hemorrhagic tendency. In 574 hemarthroses in 45 patients, the regional weather events were set in relation to the hemarthroses by means of a computer program. The following weather constellations significantly increase the tendency to bleed: Regional west weather conditions combined with longer lasting ascending procedures. The weather dynamics are dominated by the warm face of weak frontal zones with formation of a warm front. The biotropic weather elements demonstrated are also shown to be biotropic in other disease processes.

Germany, West↗
PubMed1976

[Cytological examinations of synovial for judgement arthrotic conditions at knee joint (author's transl)].

Even if you use very refinded methods of investigation, there might be some difficulties in the estimation of praearthrotic and arthrotic conditions in the knee joint, especially under the aspect of differential diagnostical considerations. In those cases all the diagnostical measures available should be applied. In this paper the value and power of evidence of cytological investigations with arthrotic alterations of the knee joint are checked. For this purpose some synovial liquid was punctured in 25 cases--chosen out of a total number of 140--of operations (mostly on behalf of suspectes meniscopathy). The liquid was worked up in the method found by Vojtisek and analysed. It was possible to correlate the thus found results (analysis of the differential cell formation) with the findings which were objectively gained during the operation. Thereby you could see that the macroscopically visible changes of the synovialis, the menisci and the cartilage, which were divided into 5 groups regarding their form and their degree of significance, allowed a comparison with the findings of the differential cell formations. It is possible to attach certain forms of the differential cell formation to varying macroscopic-anatomic changes of the knee joint. The results are thoroughly discussed.

Cytodiagnosis↗
PubMed1975

[Distribution of the isoenzym lactatdehydrogenase in human cartilage (author's transl)].

The analysis of the lactatdehydrogenase (LDH)-isoenzym patterns in normal human cartilage will be of valuable help in the interpretation of enzymological data in cases of pathological joint changes. It may allow conclusions about the situation of the metabolism in a joint according to the particular circumstances. As so far no representative investigations into normal human cartilage have become known, the attempt has been made in this paper to evaluate the LDH-isoenzym pattern in normal cartilage. The result indicates that in normal human joint cartilage the LDH-5 dominates with an average of 75.3%, whereas the LDH-4 (21.7%) and the LDH-3 (3.2%) are considerably lower. Thus the result fits well into the joint cartilage data which have been published so far.

Adolescent↗
PubMedDate not supplied

[Cell proliferation in human arthrotic joint cartilage].

OBJECTIVE: Osteoarthritic (OA) cartilage is histologically characterized by the appearance of cell clusters, which are probably generated by mitotic cell division. The aim of this study was to analyze the distribution and amount of proliferating chondrocytes in healthy and osteoarthritic cartilage systematically. MATERIALS AND METHODS: 6 normal and 43 osteoarthritic cartilage/bone samples were obtained during autopsies or total knee replacements. The cartilage specimens were stained with safranin-o and scored according to the Mankin-System. Proliferating chondrocytes were identified by immunohistochemical detection of the antigen PCNA. The number of proliferating chondrocytes was determined by counting 100 chondrocytes in each cartilage layer. RESULTS: In normal and osteoarthritic human cartilage, proliferating chondrocytes were detected. The amount of proliferating chondrocytes increased during the progression of OA cartilage changes. Expression of PCNA was determined in single chondrocytes and clustered chondrocytes. Chondrocyte cell division was activated specifically in cartilage with severe OA changes. CONCLUSIONS: Osteoarthritic chondrocytes, which are known to increase the synthesis of different matrix proteins, show the phenomenon of mitotic cells division. Whether the increased amount of proliferating chondrocytes fails to compensate the rate of apoptotic chondrocytes, or whether the generation of cell clusters represents only a fruitless repair effort is a subject of recent research. However, the induction of chondrocyte proliferation is a potential target in the treatment of OA.

Cartilage, Articular↗
PubMedDate not supplied

[Metabolic activation of chondrocytes in human osteoarthritis. Expression of type II collagen].

OBJECTIVE/METHODS: Type II collagen is the dominating collagen in articular cartilage. It is essential for the structural integrity and the biomechanical properties of cartilage. Using immunohistology and in situ hybridization we systematically analyzed the protein and mRNA-expression of type II collagen in cartilage/bone sections without any signs of osteoarthritis and osteoarthritic samples with various degrees of osteoarthritis. RESULTS: In normal articular cartilage without any histologic signs of osteoarthritis type II collagen was distributed homogeneously. An expression of the type II collagen-mRNA was not detectable in any of these samples. In cartilage sections with a roughening of the cartilage surface and a superficial loss of the safranin O staining as early histologic signs of osteoarthritis the immunohistologic staining for type II collagen was reduced in the deep cartilage zone. An expression of the type II collagen-mRNA was found in 19 of 35 preparations. This expression, however, was restricted to the middle and deep zone of cartilage. A good and reproducible correlation of the specific gene and protein expression was found in samples with more severe osteoarthritic lesions. CONCLUSIONS: Detailed information on metabolic changes and the activation of chondrocytes in osteoarthritic cartilage are important to characterize certain stages of osteoarthritis and thus identify new prognostic factors. Increasing knowledge of the factors regulating the matrix synthesis and degradation in cartilage will provide the basis for new disease modifying therapies in osteoarthritis.

Adult↗
PubMedDate not supplied

[Collagen type VI content in healthy and arthritis knee joint cartilage].

AIM OF THE STUDY: Since collagen type VI seems to play an important role in cartilage metabolism and is increased in osteoarthritis, the aim of this study was to investigate whether different stages of osteoarthritis can be characterised by the content of collagen type VI in human knee cartilage. MATERIAL AND METHODS: Collagen type VI was investigated in 148 histologically normal and 117 osteoarthritic cartilage samples from 18 different localisations of human knee joints. It was quantified in cartilage extracts using an inhibition-ELISA. RESULTS: In normal cartilage the average content of collagen type VI was 0.48 per cent of total collagens. Interestingly, there was a high variability not only in osteoarthritic, but also in normal cartilage. The statistical analysis showed significant differences between normal femoral, tibial or retropatellar cartilage samples. Therefore, normal and osteoarthritic samples from different localizations had to be compared separately. A significant increase of collagen type VI was already found in early osteoarthritic lesions. CONCLUSIONS: Inspite of a statistically significant increase of collagen type VI in osteoarthritic cartilage, the range of concentrations found in normal and osteoarthritic samples overlap. In view of the high interindividual variability, collagen type VI is not very precise in the diagnosis of early osteoarthritic lesions if used as the only marker.

Adult↗