[Permeation and hexokinase as a regulator of glycolysis of rabbit erythrocytes and reticulocytes].
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Animals↗
Biomedical subjects
G Wilke
Publications and source records attributed to G Wilke.
Analysis of meiotic chromosomes in ejaculates of infertile patients with an increased number of immature germ cells in semen samples.
Seventy-one semen samples of 60 infertile patients with a suggested high number of immature germ cells were assigned for chromosomal analysis. The sperm probes of nine patients did not contain immature germ cell stages apart from spermatids. In 22 probands we could only find early prophase stages or not readable metaphase structures. Only 25 patients could be analysed cytogenetically. The semen sample of each patient contained degenerated germ cell structures associated with desynaptic and fragmented/pulverized metaphase cells. It follows that in each analysed probe we could detect anomalies which can be attributed to errors in the gametic development process and which are possibly connected with the existing teratozoospermia and oligozoospermia. The easy practicality for cytogenetic analysis represents the most important advantage of the described method. The analysis of immature germ cell stages in semen samples makes it possible to bypass or to reduce the incidence of tests biopsies for meiotic chromosome investigation.
Chromosome Aberrations↗
Prognostic value of an automated sperm analysis in IVF or insemination therapy.
During the course of sterility treatment semenograms of 271 IVF and 316 insemination patients were carried out by two automated semen analysers. The parameters of these analyses were correlated to pregnancies resulting from the treatment. Semen samples were analysed in the ejaculate and after swim-up preparation. In addition, the swim-up suspension of IVF patients was measured again 18 h after sperm preparation. Patients were divided into three groups: (1) couples who achieved a pregnancy, (2) couples who did not achieve a pregnancy, and (3) IVF patients with no fertilization of the oocytes. Because of large standard deviations in the quality of ejaculates, the results in the IVF group show no significant differences in the semen parameters of husbands of pregnant and non-pregnant women. In contrast husbands of women with no fertilization have a significantly lower sperm motility. After swim-up preparation these differences no longer occur. A further measurement, taken 18 h later, reveals that there are no differences in the sperm parameters between the pregnant and non-pregnant group. However, the semen quality in the group with no fertilization is significantly reduced. The results of the insemination patients are similar to those of the IVF group. Thus, the results from automated sperm analysers cannot replace either the microscopic or biochemical analysis of an ejaculate and, moreover, cannot be used as prognosis for the fertilization capacity of sperms or a following pregnancy.
Autoanalysis↗