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Biomedical subjects

G Zhang

Publications and source records attributed to G Zhang.

At least 649 records · Page 36Linked to original sources
PubMed1991

[Conditional logistic regression analysis of coronary heart disease].

A case-control study on 215 pairs was carried out in Jinan. Conditional Logistic regression analysis showed that the following five factors were associated with coronary heart disease, i.e., hypertension, hypercholesterolemia and heavy smoking, serum copper and HDL-C/TC. The former three were risk factors, and the latter two were protective factors. There is remarkable dose-response relation between heavy smoking and coronary heart disease. The data analysis show that these five factors are contributory to coronary heart disease with synergism.

Case-Control Studies↗
PubMed1990

Anticonvulsant effect of N-ethylcarboxamidoadenosine against kainic acid-induced behavioral seizures in the rat prepiriform cortex.

Kainic acid (KA), microinjected unilaterally into the rat prepiriform cortex (PC), produces generalized motor seizures in a dose-dependent manner. The adenosine agonist N-ethylcarboxamidoadenosine (NECA), when co-injected with KA, protects against seizures in a dose-dependent and highly potent manner: ED50 = 25.6 +/- 2.1 pmol/rat. The seizure-suppressing effects of NECA are completely abolished by co-administration of the adenosine receptor antagonist 8-(p-sulfophenyl)theophylline (8-pSPT), suggesting that adenosine receptor activation underlies the efficacy of NECA against KA seizures. Moreover, dilazep, an effective blocker of adenosine uptake, when co-administered with KA, provides significant protection against seizures. Together, these findings suggest that adenosine receptors may play an important role in the regulation of the inhibitory neuronal circuitry of this paleocortical brain area.

Adenosine↗
PubMed1990

Patient acceptance of and satisfaction with an external negative pressure device for impotence.

Patient acceptance of and satisfaction with an external negative pressure device as a treatment for impotence were retrospectively analyzed among 100 men. The over-all satisfaction rate was 68%. Reasons for dissatisfaction with and discontinuing the use of the device included premature loss of penile tumescence and rigidity, pain or discomfort either during application of suction or during intercourse and inconvenience. Negative pressure therapy is an effective treatment for impotence of various etiologies and should be among treatment options offered to the impotent patient.

Consumer Behavior↗
PubMed1990

The structure of the pseudorabies virus genome at the end of the inverted repeat sequences proximal to the junction with the short unique region.

The complete nucleotide sequence is presented of the 2 x 67 kbp BamHI-EcoRV portion of the BamHI 10 fragment of the pseudorabies virus (PRV) genome (strain Ka) containing sequences upstream of the previously reported protein kinase gene, and completing the sequence of this 4008 bp fragment. It is predicted to contain a gene designated RSp40, homologous to gene US1 of herpes simplex virus type 1 (HSV-1), with the potential to encode a protein of 364 amino acids. Analysis of PRV mRNA synthesized in the presence and absence of cycloheximide indicated that, in contrast to its HSV-1 homologue, the PRV gene RSp40 does not specify an immediate-early mRNA. Between the RSp40 gene and the protein kinase gene are two reiterated sequences: one containing 11 tandem copies of a 35 nucleotide sequence and the other containing nine tandem copies of a 10 nucleotide sequence. The BamHI 10 and the BamHI 12 fragments of PRV contain the junctions between the short unique (US) and short repeat (RS) regions of the PRV genome. The nucleotide sequence of that portion of the BamHI 12 fragment containing US sequences was determined so that, by comparison with the nucleotide sequence of the BamHI 10 fragment, the junction between the US and RS regions could be defined. In BamHI 10 this was found to be at a point between the two reiterated sequences (which are in the RS region) and the protein kinase gene (which is in the US region). The organization of this region of the PRV genome is compared to that of other alphaherpesviruses.

Amino Acid Sequence↗
PubMed1990

The protein kinase encoded in the short unique region of pseudorabies virus: description of the gene and identification of its product in virions and in infected cells.

We have cloned and determined the nucleotide sequence of a gene, pk, that lies immediately upstream from the gene encoding glycoprotein X in the short unique region of the alphaherpesvirus, pseudorabies virus (PRV). The gene has the potential to encode a protein of 334 amino acids, and is related to gene US3 of herpes simplex virus type 1 (HSV-1), which has been shown to encode a protein kinase. The predicted amino acid sequence encoded by the PRV pk gene is homologous to the corresponding sequence encoded by the HSV-1 US3 gene in the C-terminal catalytic domain, but diverges markedly in the N-terminal domain. As with HSV-1, the mRNA for the pk gene appears to be 3' coterminal with that for the glycoprotein downstream. An antiserum was raised against a protein generated from the fusion of part of the PRV pk catalytic domain with Escherichia coli beta-galactosidase. This specifically reacted with a previously described physically homogeneous protein kinase, PRV-PK, isolated from hamster fibroblasts lytically infected with PRV. Although the majority of the PRV-PK is found in the cytoplasm, some was also detected in purified PRV virions by using the same antibody; a similar distribution was found for the HSV-1 protein kinase, using an antiserum raised against the corresponding HSV-1 fusion protein. When presented with heatinactivated virions, purified PRV-PK (in common with certain cellular protein kinases also present in the virion) was able to phosphorylate in vitro the major virion phosphoprotein phosphorylated in vivo.

Amino Acid Sequence↗
PubMed1990

Kinetics of Aluminum Uptake in Triticum aestivum L: Identity of the Linear Phase of Aluminum Uptake by Excised Roots of Aluminum-Tolerant and Aluminum-Sensitive Cultivars.

The identity of a linear phase of aluminum (Al) uptake in Triticum aestivum was investigated by analysis of the kinetics of Al uptake by excised roots and purified cell wall fractions. Classical interpretation of kinetic data suggests that a linear phase of uptake with time reflects uptake across the plasma membrane; however, in studies with Al the possibility that the linear phase of uptake includes accumulation of Al in both the symplasm and the apoplasm has not been discounted. In our experiments, we observed a linear phase of Al uptake at both ambient and low temperatures, although the rate of uptake at 0 degrees C was 53 to 72% less than at 23 degrees C, depending on cultivars. This nonsaturable phase of uptake at low temperature suggests that a portion of the linear phase of Al uptake is nonmetabolic. Furthermore, analysis of Al in cell wall fractions isolated from excised roots pretreated with Al suggests that the linear phase of uptake includes a cell wall component. When excised roots were pretreated with Al, accumulation of Al in purified cell wall material included a linear phase that could not be desorbed with a 30 minute wash in citrate. The rates of linear-phase accumulation of Al by cell wall material and cell contents were similar. In contrast, the linear phase of in vitro uptake of Al by purified cell wall material was completely desorbed by a 30 minute wash with citrate. These results suggest that the linear phase of Al uptake observed in excised roots of T. aestivum included metabolism-dependent binding of Al in apoplasm.

Journal Article↗
PubMed1990

Experimental treatment of benign prostatic hyperplasia with transurethral balloon dilation of the prostate: preliminary study in 73 humans.

A prospective noncontrolled study of the safety and potential efficacy of transurethral balloon catheter dilation of the prostate (TUDP) in the treatment of benign prostatic hyperplasia (BPH) was performed in 73 subjects with moderate to severe symptoms and signs of prostatism who were selected on the basis of a quantitative symptom score (SS), uroflowmetry measurements, and residual urine volume. Seven patients had urinary retention. Mean age was 69.6 years (range, 59-95 years). TUDP was successfully accomplished in 70 patients (96%). There were no significant complications. Mean follow-up was 16.2 months (range, 6-36 months). Forty-six patients (66%) showed improved SS at the most recent follow-up. In 24 patients (34%) SS was unimproved, necessitating prostatectomy in 17 subjects (24%). Reduction in mean residual urine volume was not statistically significant. Only 38% of patients with median lobe enlargement showed improvement in SS, compared with 74% for the others. The authors conclude that TUDP is safe and shows promising effectiveness and that the ultimate demonstration of effectiveness requires a controlled clinical trial.

Aged↗
PubMed1990

Gelatinolytic proteinase activities in human seminal plasma.

Proteinase activities in human seminal plasma were detected using gelatin-containing sodium dodecyl sulphate-polyacrylamide gel electrophoresis zymography. Three prominent bands of activity of Mr 60,000, 66,000 and 90,000 were observed as well as 9 other bands of less intensity (34,000-158,000). These proteinases were dependent upon calcium for optimal activity, did not hydrolyse casein, and were predominantly in the soluble portion of seminal plasma. Examination of seminal plasma of men with different sperm concentrations, split ejaculates, and prostatic secretions indicated that the prostate gland was a source of most of these activities. Proteinase activities of Mr 34,000, 37,000, 82,000 and 120,000 were expressed more frequently in seminal plasma from normozoospermic men than from seminal plasma of oligo- or azoospermic men, indicating that they may also arise from spermatozoa in the semen sample. The proteinases of Mr 60,000 and 66,000 were found in all seminal plasmas whereas there was variation in the expression of the other molecular forms of enzyme, even in the normozoospermic samples. There are multiple forms of gelatinolytic proteinase activities in human seminal plasma which appear to arise from multiple sources in the reproductive tract including the Cowper's/urethral glands, the prostate gland, seminal vesicle and/or spermatozoa. Their function(s) in semen remains to be established.

Electrophoresis, Polyacrylamide Gel↗
PubMed1990

Two types of rhythmical jaw movements evoked by stimulation of the rat cortex.

Two different patterns of rhythmical jaw movements (RJM) resembling masticatory movements were evoked by stimulation of the cerebral cortex in the rat. The first type of RJM was evoked from the primary jaw motor area, and it always began with a sustained jaw-opening and consisted of high frequency (5-7 Hz) simple opening-closing movements of the jaw. The anterior digastric electromyographic (EMG) activity showed discharges apparently time-locked to each stimulus pulse. The second type of RJM was evoked from the ventral part of the insular cortex. In contrast to the first type of RJM, the second type started with a jaw closing movement in many cases. This type of RJM had large and complex lateral and sagittal movements and the frequency of RJM was low (3-4 Hz). The digastric EMG activity did not show the stimulus-locked component. The pattern of these RJM did not depend on the intensity or frequency of stimulation, but on the site stimulated. These two cortical RJM areas appeared to work independently from each other, because ablation of one area did not affect the pattern of the RJM evoked from the other cortical area.

Animals↗
PubMed1990

[Reversed anterolateral thigh island flap and myocutaneous flap transplantation].

The blood supply of free anterolateral thigh flap and myocutaneous flap comes from the descending branch of the lateral femoral circumflex artery. On the basis of anatomical data, the descending branch coincides with the lateral superior genicular artery. Therefore, the authors suggested reversed anterolateral thigh island flap and myocutaneous flap transplantation. The blood supply of the reversed island flap, is from the lateral superior genicular artery. The flap seemed to have a constant vascular anatomy with a long pedicle and a large diameter, so that dissection of the flap can be accomplished easily. Moreover, donor defects were usually closed primarily without the need of skin-grafting. Since 1985, 4 cases of soft tissue defect around the knee joint have been repaired with reversed anterolateral thigh island flap and myocutaneous flap. All flaps survived completely and obtained satisfactory results. In this paper, the anatomy, design and operative technique of the flap are discussed.

Adolescent↗
PubMed1990

Transmitter release modulated by isoprenaline in the dog isolated mesenteric vein.

1. The effects of isoprenaline on the release of transmitter substances from perivascular adrenergic nerves were estimated from the excitatory junction potential (ejp) and outflow of noradrenaline in the dog mesenteric vein. 2. Isoprenaline increased the ejp amplitude and decreased the evoked release of noradrenaline. Yohimbine potentiated the former and converted the latter to an increased outflow. 3. Therefore, stimulation of prejunctional beta-adrenoceptor by isoprenaline increases the release of noradrenaline from perivascular adrenergic nerves. 4. Possible involvement of prejunctional alpha-adrenoceptors in the isoprenaline-induced modulation of transmitter release was also suggested.

Action Potentials↗
PubMed1990

[DNA polymorphisms and haplotypes in the apoAI-CIII gene region and coronary heart disease].

Polymorphisms and haplotypes identified by DNA Restriction Fragment Length Polymorphisms in the apoAI-CIII gene region were investigated both in 33 Chinese patients with coronary heart disease and 50 unrelated healthy subjects, using genomic DNA hybridization analysis with a 1.0 kb fragment of the human apoAI gene cDNA. The frequencies of the S2 and M2 alleles were 0.167 and 0.22 in healthy subjects, 0.11 and 0.24 in patients respectively. There were no differences in the frequencies of the S2 and M2 alleles between patients and healthy subjects. Since none of the subjects had the genotypes, S1S2M1M1, S2S2M1M2 and S2S2M1M1, all of the S2 alleles were considered to be associated with the M2 alleles at least in Chinese population. The alleles identified by the apoCIII Sst-I and apoAI Msp-I polymorphisms are in linkage disequilibrium. The individuals with the haplotype S1-M2 were significantly increased in patients compared with the ones in healthy subjects (26% versus 4.6%; chi 2 = 6.91, d.f = 1, P less than 0.01). The results of the present study suggest that both the apoCIII Sst-I and apoAI Msp-I polymorphisms may be a useful genetic marker for the analysis of apoAI-CIII gene complex and the haplotype S1-M2 may be a useful linkage marker for the putative atherogenic gene in Chinese.

Apolipoprotein A-I↗
PubMed1990

[Angiographic study of osteosarcoma and fibrosarcoma of the jaw].

It has been well known that the sarcomas of jaw are very vascularized, but reports on the angiography of jaw sarcomas were very few. Based on the preoperative angiography of 3 cases of jaw bone sarcomas and arteriography of their specimens, it has been noticed that the supplying arteries of the mandible ramus sarcoma can be directly from the external carotid arteries; and the supplying arteries can be from inferior alveolar artery in sarcomas of the body of mandible. The tumors are more vascularized than the surrounding normal tissues, and the direction of the arteries can be from central to peripheral in the tumors, which are different from the long bone sarcomas. The vascularization and the retarded blood flow in the tumor are the significant anatomic bases for local perfusion chemotherapy of jaw sarcomas.

Adolescent↗
PubMed1990

[The influence of deep or shallow acupuncturing "huantiao" acupoint upon the analgesia and histochemical components of locus coeruleus].

Fifty-four healthy male, adult rats were divided at random into three groups: deep and shallow acupuncture groups and the controls. After acupuncturing, the pain threshold were measured and the animals were sacrificed for histochemical examination in locus coeruleus. The pain threshold of deep acupunctured animals was elevated but that of shallow acupunctured and controls were not. The difference between them was significantly. The amount of AChE and RNA of perikaryons in locus coeruleus of mentioned three groups were measured with micropiotometer. The reaction of AChE and RNA in deep acupuncture group increased significantly in comparison with that of control as well as shallow acupuncturing group. These reactivities of the latter two groups were similar to each other. The results indicated that the effect of acupuncture might be involved in the stimulating of the deeper structure of acupuncture point.

Acupuncture Analgesia↗