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Biomedical subjects

G de Groot

Publications and source records attributed to G de Groot.

At least 19 recordsLinked to original sources
PubMed2003

Effects of noise on the performance of rats in an operant discrimination task.

Two experiments examined the effect of the presentation of an irregular, moderate intensity auditory stimulus ('noise') on the performance of rats in an operant discrimination task. In Experiment 1, rats first learned to press a lever in the presence of a visual stimulus but not in its absence. Discrimination performance was impaired during subsequent exposure to noise. In Experiment 2, different groups of rats learned the discrimination task under a noise or a no-noise condition. Thereafter, all rats were tested under each noise condition. Discrimination performance was best when the noise condition at test was identical to the noise condition at training. These results were discussed in the framework of arousal, distraction, generalization decrement, and contextual occasion setting. They point to the necessity of using a 2x2 factorial design in human and animal research on noise effects, with noise condition at training (noise present or absent) and noise condition at test (noise present or absent) as factors.

Journal Article↗
PubMed2002

Differences in leg muscle activity during running and cycling in humans.

Delta (delta) efficiency is defined as the ratio of an increment in the external mechanical power output to the increase in metabolic power required to produce it. The purpose of the present study was to investigate whether differences in leg muscle activity between running and cycling can explain the observed difference in delta efficiency between the two activities. A group of 11 subjects performed incremental submaximal running and cycling tests on successive days. The delta efficiencies during running and cycling were based on five exercise stages. Electromyograph (EMG) measurements were made of three leg muscles (gastrocnemius, vastus lateralis and biceps femoris). Kendall's correlation coefficients between the mean EMG activity and the load applied were calculated for each muscle, for both running and cycling. As expected, the mean delta efficiency during running (42%) was significantly greater than that during cycling (25%). For cycling, all muscles showed a significant correlation between mean EMG activity and the load applied. For running, however, only the gastrocnemius muscle showed a significant, but low correlation ( r=0.33). The correlation coefficients of the vastus lateralis and biceps femoris muscles were not significantly different from 0. The results were interpreted as follows. In contrast to cycling, which includes only concentric contractions, during running up inclines eccentric muscle actions play an important role. With steeper inclines, more concentric contractions must be produced to overcome the external force, whereas the amount of eccentric muscle actions decreases. This change in the relative contribution of concentric and eccentric muscle actions, in combination with the fact that eccentric muscle actions require much less metabolic energy than concentric contractions, can explain the difference between the running and cycling delta efficiency.

Adult↗
PubMed2000

Physiological responses that account for the increased power output in speed skating using klapskates.

The present study investigates which physiological sources support the increase in mechanical power output (W out) that can be obtained using klapskates in speed skating. It was hypothesized that the increase in W out could be achieved through an increase in gross efficiency or an increase in aerobic power (W aer). Six speed skaters performed a submaximal and maximal 1,600-m skating test with both klapskates and conventional skates, to measure gross efficiency and maximal W aer during speed skating. The rate of oxygen uptake (VO2) and post-exercise blood lactate concentrations ([La]) were measured and video recordings were made. W aer was calculated from VO2. W out was derived from the power needed to overcome air and ice friction. Gross efficiency was calculated as the ratio of W out and W aer. In the maximal tests, the subjects skated faster with klapskates compared to conventional skates (10.0 vs 9.6 m x s(-1)). They sustained the resulting higher W out with klapskates with an equal VO2. [La] was, however, 1.7 mmol x l(-1) higher when klapskates were used, which might reflect an increase in anaerobic power. During the submaximal tests the skaters generated equal W out with both types of skate. Although not statistically significant, VO2 and W aer were, on average, lower when klapskates were used compared to conventional skates [mean (SD) 0.3 (0.43) l x min(-1), 105 (143) W]. Despite the lack of a statistically significant difference in W aer, gross efficiency was shown to be significantly higher with klapskates compared to conventional skates (16.3% vs 14.8%, P = 0.02). We conclude that the increase in W out when the subjects were using klapskates could be explained by an increase in gross efficiency rather than an increase in W aer.

Energy Metabolism↗
PubMed2000

Push-off mechanics in speed skating with conventional skates and klapskates.

PURPOSE: Personal and world records in speed skating improved tremendously after the introduction of the klapskate, which allows the foot to plantar flex at the end of the push-off while the full blade continues to glide on the ice. The purpose of this study was to gain insight into the differences in skating technique with conventional versus klapskates and to unveil the source of power enhancement using klapskates. METHODS: Ten elite speed skaters skated four 400-m laps at maximal effort with both conventional and klapskates. On the straight high-speed film, push-off force and EMG data were collected. An inverse dynamics analysis was performed in the moving reference plane through hip, knee, and ankle. RESULTS: Skating velocity increased 5% as a result of an increase in mean power output of 25 W when klapskates were used instead of conventional skates. The increase in mean power output was achieved through an 11-J increase in work per stroke and an increase in stroke frequency from 1.30 to 1.36 strokes x s(-1). The difference in work per stroke occurs during the final 50 ms of the push-off. This is the result of the ineffective way in which push-off forces are generated with conventional skates when the foot rotates about the long front end of the blade. No differences in muscle coordination were observed from EMG. CONCLUSION: A hinge under the ball of the foot enhances the effectiveness of plantar flexion during the final 50 ms of the push off with klapskates and increases work per stroke and mean power output.

Adult↗
PubMed1999

Determining propulsive force in front crawl swimming: a comparison of two methods.

To evaluate the propulsive forces in front crawl arm swimming, derived from a three-dimensional kinematic analysis, these values were compared with mean drag forces. The propulsive forces during front crawl swimming using the arms only were calculated using three-dimensional kinematic analysis combined with lift and drag coefficients obtained in fluid laboratories. Since, for any constant swimming speed, the mean propulsive force should be equal to the mean drag force acting on the body of the swimmer, mean values of the calculated propulsive forces were compared with the mean drag forces obtained from measurements on a Measuring Active Drag (MAD) system. The two methods yielded comparable results, the mean difference between them being only 5% (2 N). We conclude that propulsive forces obtained from three-dimensional kinematic analysis provide realistic values. The calculation of the propulsive force appears to be rather sensitive to the point on the hand at which the velocity is estimated and less sensitive to the orientation of the hand.

Adult↗
PubMed1998

Gastro-oesophageal reflux disease in primary care: an international study of different treatment strategies with omeprazole. International GORD Study Group.

OBJECTIVE: To assess the efficacy of omeprazole in patients presenting with troublesome reflux symptoms. DESIGN: Randomized, double-blind, parallel-group, placebo-controlled comparison. SETTING: Primary care. SUBJECTS: Patients were recruited using a symptom-based questionnaire for diagnosis of gastro-oesophageal reflux disease. INTERVENTIONS: After endoscopy, patients without endoscopic oesophagitis were randomized to omeprazole 20 mg (Ome20), omeprazole 10 mg (Ome10) or placebo once daily for 4 weeks (n = 261) and those with oesophagitis (except circumferential/ulcerative) were randomized to receive either Ome20 or Ome10 once daily for 4 weeks (n = 277). Patients not symptom-free at 4 weeks received open treatment with Ome20 once daily for a further 4 weeks. Those symptom-free at 4-8 weeks were followed up for 6 months off treatment, to see whether their symptoms recurred. MAIN OUTCOME MEASURE: Complete upper GI symptom relief during week 4 on Ome20 or Ome10 in patients with or without endoscopic oesophagitis. RESULTS: Forty one percent of all patients on Ome20 and 35% on Ome10 reported complete relief from upper GI symptoms during week 4, whilst 73% of the patients on Ome20 and 62% on Ome10 obtained sufficient control. Complete relief during week 4 was reported by 19% of endoscopy-negative patients on placebo, and sufficient control by 35%. Endoscopic healing at 4 weeks occurred in 76% of oesophagitis patients on Ome20 and in 56% on Ome10. After 6 months off treatment, 90% of patients with oesophagitis and 75% of endoscopy-negative patients reported symptomatic relapse. CONCLUSION: Both 10 mg and 20 mg of omeprazole gave effective relief of symptoms, although 20 mg gave superior healing in patients with oesophagitis. After cessation of treatment, symptomatic relapse was rapid and frequent in both endoscopy-positive and endoscopy-negative patients.

Adolescent↗
PubMed1997

Response of sensitive and resistant IgM immunocytomas to cis-diamminedichloroplatinum (II) does not correlate with the platination level or with the formation or removal of DNA adducts.

An IgM immunocytoma cell line sensitive to cis-diamminedichloroplatinum(II) (CDDP) and a subline with acquired resistance were grown in LOU/M rats. In a previous study with such rats that had been treated with a high dose of CDDP (10 mg/kg) the tumors did not show differences in cellular platinum content or DNA-adduct levels, either immediately after treatment or 24 h later. Recently, this high dose was found to overcome resistance. Therefore, the study was repeated with a 10-fold lower dose (1 mg/kg, i.v.). At 1 and 24 h after treatment, tumor and kidney tissue were assayed for cellular platinum (atomic absorption spectroscopy, AAS) and DNA platination (immunochemical detection of the four CDDP-DNA adducts). The results were compared with previous data. All tissues showed a linear response to dose with regard to platinum uptake as well as adduct formation, with no quantitative difference being seen between the tumors. Also the relative occurrence of the four adducts was very similar. Between 1 and 24 h, in tumors a substantial decrease occurred in both platinum content and adduct level; the kidneys showed little reduction, if any. At the lower CDDP dose a somewhat larger loss of platinum and removal of DNA adducts was observed for the resistant tumor, but these differences could be explained by "dilution", as this tumor continues to grow after low-dose treatment (about 20% within 24 h). Since the strong difference observed between the tumors in sensitivity to CDDP cannot be attributed to differences in CDDP uptake, efficiency of adduct formation, or repair capability, other mechanisms are held responsible.

Animals↗
PubMed1995

The control of mono-articular muscles in multijoint leg extensions in man.

1. Movements often require control of direction and a magnitude of force exerted externally on the environment. Bi-articular upper leg muscles appear to play a unique role in the regulation of the net torques about the hip and knee joints, necessary for the control of this external force. 2. The aim of this study was to test the hypothesis that the mono-articular muscles act as work generators in powerful dynamic leg extensions, which means that they should be activated primarily in the phases during which they can contribute to work, irrespective of the net joint torques required to control the external force. 3. Cycling movements of six trained subjects were analysed by means of inverse dynamics, yielding net joint torques as well as activity patterns and shortening velocities of four mono- and four bi-articular leg muscles. 4. The results show that the mono-articular muscles exert force only in the phase in which these muscles shorten, whereas this appears not to be the case for the bi-articular muscles. 5. Reciprocal patterns of activation of the rectus femoris and hamstring muscles appear to tune the distribution of net joint torques about the hip and knee joints, necessary to control the (changing) direction of the force on the pedal. 6. An analysis of running in man and additional related literature based on animal studies appears to provide further support for the hypothesis that mono- and bi-articular muscles have essentially different roles in these powerful multijoint leg extension tasks.

Bicycling↗
PubMed1995

Hydrodynamic drag and lift forces on human hand/arm models.

Forces acting on the forearm and hand during swimming can be decomposed into drag forces and lift forces. In this study drag and lift forces were measured on two models of a human hand and forearm when towed in a towing tank. To compare the results of models with different size at different velocities force data were normalized to drag and lift coefficients (Cd and Ct). Influence of the orientation of the model with respect to the flow, velocity, size of the model and the relative contribution of the hand and forearm on Cd and Ct were studied. The orientation of the model with respect to the line of motion was varied by rotating the models around three axes, and quantified using the angle of pitch (AP: the angle between the hand plane and flow) and the sweep-back angle (SB: the orientation of the flow vector when projected on the hand plane). Cd was maximal when the palm of the hand is almost perpendicular to the flow (AP = 65 degrees, SB = 342 degrees). Ct shows maximal values at two different orientations: with the hand in a thumb-leading position, AP = 31 degrees, SB = 358 degrees, and with the hand in a little finger-leading position, AP = 48 degrees, SB = 193 degrees. The orientation of the hand was very critical in generating lift forces. By contrast, the influence of velocity and size of the model on the values of Cd and Ct was limited.(ABSTRACT TRUNCATED AT 250 WORDS)

Biomechanical Phenomena↗
PubMed1995

Air friction and rolling resistance during cycling.

To calculate the power output during actual cycling, the air friction force Fa and rolling resistance Fr have to be known. Instead of wind tunnel experiments or towing experiments at steady speed, in this study these friction forces were measured by coasting down experiments. Towing experiments at constant acceleration (increasing velocity) were also done for comparison. From the equation of motion, the velocity-time curve v(t) was obtained. Curve-fitting procedures on experimental data of the velocity v yielded values of the rolling resistance force Fr and of the air friction coefficient k = Fa/v2. For the coasting down experiments, the group mean values per body mass m (N = 7) were km = k/m = (2.15 +/- 0.32) x 10(-3)m-1 and ar = Fr/m = (3.76 +/- 0.18) x 10(-2)ms-2, close to other values from the literature. The curves in the phase plane (velocity vs acceleration) and the small residual sum of squares indicated the validity of the theory. The towing experiments were not congruent with the coasting down experiments. Higher values of the air friction were found, probably due to turbulence of the air.

Air↗
PubMed1995

The start in speed skating: from running to gliding.

The purpose of this study was to describe the push-off kinematics in speed skating using three-dimensional coordinates of elite male sprinters during the first part of a speed skating sprint. The velocity of the mass center of the skater's body VC, is decomposed into an "extension" velocity component VE, which is associated with the shortening and lengthening of the leg segment and a "rotational" velocity component Vr, which is the result of the rotation of the leg segment about the toe of the skate. It can be concluded that the mechanics of the first strokes of a sprint differ considerably from the mechanics of strokes later on. The first push-offs take place against fixed location on the ice. In these "running-like" push-offs the contribution of Vr in the forward direction is larger than the extension component Ve. Later on, the strokes are characterized by a gliding push-off in which Ve increases. In these gliding push-offs no direct relation exists between forward velocity of the skater and the extension in the joints. This allows skaters to obtain much higher velocities than can be obtained during running.

Acceleration↗
PubMed1994

Power, muscular work, and external forces in cycling.

Cycling performance is affected by the interaction of a number of variables, including environment, mechanical, and human factors. Engineers have focused on the development of more efficient bicycles. Kinesiologists have examined cycling performance from a human perspective. This paper summarizes only certain aspects of human ergonomics of cycling, especially those which are important for the recent current research in our departments. Power is a key to performance of physical work. During locomotion an imaginary flow of energy takes place from the metabolism to the environment, with some efficiency. The 'useful' mechanical muscle power output might be used to perform movements and to do work against the environment. The external power is defined as the sum of joint powers, each calculated as the product of the joint (net) moment and angular velocity. This definition of external power is closely related to the mean external power as applied to exercise physiology: the sum of joint powers reflects all mechanical power which in principle can be used to fulfil a certain task. In this paper, the flow of energy for cycling is traced quantitatively as far as possible. Studies on the total lower limb can give insight into the contribution of individual muscles to external power. The muscle velocity (positive or negative) is obtained from the positions and orientations of body segments and a bar linkage model of the lower limb. The muscle activity can be measured by electromyography. In this way, positive and negative work regions in individual muscles are identified. Synergy between active agonistic/antagonistic muscle groups occurs in order to deliver external power. Maximum power is influenced by body position, geometry of the bicycle and pedalling rate. This has to be interpreted in terms of the length-tension and force-velocity-power relationships of the involved muscles. Flat road and uphill cycling at different saddle-tube angles is simulated on an ergometer. The measured pedal forces (magnitude and direction) are only dependent on the intersegmental orientation of saddle tube, crank position, upper and lower leg, and foot. The changed direction of the gravitational force with respect to the saddle-tube does not interfere with the co-ordinated force production pattern. During locomotory cycling at constant speed the external power is mainly used to overcome the aerodynamic friction force. This force and the rolling resistance are determined by coasting down experiments, yielding the external power.(ABSTRACT TRUNCATED AT 400 WORDS)

Bicycling↗
PubMed1994

Longitudinal development of young talented speed skaters: physiological and anthropometric aspects.

A longitudinal analysis of a group of speed skaters was done to identify the performance-determining factors for a successful speed skating career. This paper presents both the physiological and anthropometric results of this longitudinal study. Twenty-four athletes from the Dutch National Junior Speed Skating Team were followed from age 16-17 yr to age 20-21 yr. During the development from junior to senior speed skater, a number of anthropometric and physiological variables changed. There were no differences between successful and unsuccessful speed skaters from an anthropometric perspective; consequently, it was not possible to distinguish successful from unsuccessful athletes on anthropometric grounds. The longitudinal data showed that at a younger age the successful speed skaters had similar oxygen consumption, mechanical efficiency, and power output values compared with the unsuccessful speed skaters. Later in the study, successful speed skaters distinguished themselves by the ability to produce higher power output values. There were no anthropometric or physiological relationships found in this study on which performance at the age of 20-21 yr could be predicted with measurements at a junior age.

Adolescent↗
PubMed1994

Optimisation of sprinting performance in running, cycling and speed skating.

Sprinting performances rely strongly on a fast acceleration at the start of a sprint and on the capacity to maintain a high velocity in the phase following the start. Simulations based on a model developed in which the generation of metabolic power is related to the mechanical destinations of power showed that for short-lasting sprinting events, the best pacing strategy is an all out effort, even if this strategy causes a strong reduction of the velocity at the end of the race. Even pacing strategies should only be used in exercises lasting longer than 80 to 100 seconds. Sprint runners, speed skaters and cyclists need a large rate of breakdown of energy rich phosphates in the first 4 to 5 seconds of the race (mechanical equivalent > 20 W/kg) in order to accelerate their body, and a power output of more than 10 W/kg in the phase following the start to maintain a high velocity. Maximal speed in running is mainly limited by the necessity to rotate the legs forwards and backwards relative to the hip joint. The acceleration phase, however, relies on powerful extensions of all leg joints. Through a comparison of the hindlimb design of highly specialised animal sprinters (as can be found among predators) and of long distance animal runners (as found among hoofed animals), it is illustrated that these 2 phases of a sprint rely on conflicting requirements: improvement of maximal speed would require lower moments of inertia of the legs whereas a faster acceleration would require the involvement of more muscle mass (not only of the hip and knee extensors but also of the plantar flexors). Maximal speed in cycling and speed skating is not limited by the necessity to move leg segments but rather on air friction and rolling or ice friction. Since the drag coefficients found for speed skaters and cyclists (about 0.8) are considerably higher than those of more streamlined bodies, much progress can still be expected from the reduction of air friction. Speed skaters and especially cyclists show much smaller accelerations during the start than do sprint runners. Skaters might try to improve their very first push off by developing a start technique that allows a much more horizontally directed propulsive force. The small propulsive force at the onset of a cycling sprint is due to the gearing system.(ABSTRACT TRUNCATED AT 400 WORDS)

Acceleration↗
PubMed1994

The platelet adhesion capacity to subendothelial matrix and collagen in a flow model during storage of platelet concentrates for 7 days.

The influence of storage of platelet concentrates (PC) on the adhesion capacity of platelets was studied. Twenty-four PC, 12 prepared by the buffy coat (BC) method and 12 by the platelet-rich plasma (PRP) method, were stored for 7 days at room temperature. On days 1, 3 and 7 of storage, the platelet adhesion capacity to subendothelial matrix (SEM) and collagen was studied in a rectangular perfusion system under flow conditions in conjunction with the platelet aggregation capacity after stimulation and the adenine nucleotide content. The platelet adhesion capacity to collagen was constant until day 3 of storage and decreased to about 80% of the starting value on day 7 of storage. The adhesion capacity to SEM, however, had already decreased on day 3 to about 75% of the value of day 1 and was even more decreased on day 7 to about 45% of the starting value. On day 1, platelets prepared by the BC method displayed a higher adhesion capacity to collagen and a higher aggregation capacity after stimulation by collagen alone or in combination with ADP, compared to platelets prepared by the PRP method. No other significant differences in adhesion or aggregation capacity were observed between the PC prepared by the two different methods. Both platelet adhesion and aggregation response decreased during storage, as did the total adenine nucleotide content. This study shows that platelet function, as measured by the aggregation and adhesion capacity, of platelets prepared by the PRP method is more severely impaired during the first 3 days of storage as compared to the function of platelets prepared by the BC method.

Blood Platelets↗
PubMed1993

Tolerance and withdrawal after chronic lorazepam treatment in rats.

The purpose of the study was to develop an animal model for benzodiazepine tolerance and dependence on the basis of oral administration, using lorazepam as the test drug. We have used the continuous measurement of locomotor activity in home cages to obtain a narrow estimation of the time course of withdrawal related hyperactivity as an observer-independent symptom. Acute administration of lorazepam (9.5-37.5 mg/kg body weight/day) resulted in the first week in a dose-dependent muscle relaxation on the accelerod and sedation in the open field. The most striking manifestation of sedation, however, was the decrease of nocturnal locomotor activity in home cages. After 5 weeks of administration tolerance to the sedative effect had developed. In a second study, using a lower dose range (2.5-9.5 mg/kg body weight/day), a decrease of nocturnal locomotor activity was also observed as was the development of tolerance. The latter can be partly explained by dispositional tolerance, i.e., decreased serum concentrations after administration of lorazepam for more than 2 weeks. Withdrawal of lorazepam in the experiment using high doses led to three symptoms, i.e., a decrease in food intake, loss of body weight and an increase in daytime locomotor activity. The decrease in food intake and the loss of body weight were maximal on the first day of withdrawal. The increase in the daytime locomotor activity was present in the high dose experiment only, with a maximum on days 2-3 and a duration of at least 1 week. The increase however, was not dependent on the dose previously administered. The symptoms 'loss of body weight' and 'decrease in food intake' appeared to be more sensitive in benzodiazepine withdrawal: they were dose dependently present over the whole dosage range (2.5-37.5 mg/kg body weight/day). It is concluded that the model represents a sensitive model to measure lorazepam tolerance and dependence in animals. Comparative studies with other benzodiazepines are in progress.

Animals↗
PubMed1993

Pharmacokinetic differences of desmethyldiazepam in three outbred Wistar strains related to differences in liver enzyme activities.

Various strains of rats differ in their sensitivities towards benzodiazepines suggesting that variations in biological features are responsible for these differences. All reports concern inbred strains. We have studied male rats of three outbred Wistar strains with regard to the pharmacokinetics of desmethyldiazepam given as a single intravenous dose. The elimination half-life of desmethyldiazepam was longer in the Riv:TOX(M) (from the National Institute of Public Health and Environmental Protection) and in the Cpb:WU strain than in the Crl:(WI)BR strain. This strain difference in elimination of desmethyldiazepam may be related to differences in liver enzyme activities.

Animals↗