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Biomedical subjects

G von Bally

Publications and source records attributed to G von Bally.

At least 19 recordsLinked to original sources

[Investigation of influencing variables on the computer-aided simulation of contacts in dynamic occlusion based on optically digitized plaster casts].

In dentistry, mechanical articulators with which mandibular movements can be reproduced in dentals casts play a major role. Commonly used semiadjustable articulators, however, have major limitations: On the one hand, the movement of the mandible is not reproduced exactly, on the other, they do not provide time-related information on jaw movement. Both problems can be solved by replacing the mechanical articulator by a digital simulation ("virtual articulator") based on digitized plaster casts and electronically recorded masticatory movements. We present a system for the 3D measurement of plaster casts in a skull-related, anatomical coordinate system using the fringe projection technique, and electronically recorded condylar movements. Using numerical algorithms, the contacts between upper and low jaw, and the angle of rotation of the temporomandibular joint can be computed for each movement in dynamic occlusion. Taking the data recorded from a patient as an example, the influence of the accuracy of the digitization of plaster casts on the computation of the rotation of the temporomandibular joint is discussed in relation to the anatomy of the masticatory apparatus.

Computer Simulation↗

[Computer-assisted development of epitheses after optical recording of facial defects].

A major drawback of conventional impression techniques used for customizing facial prostheses is the fact that pressure applied deforms soft tissue, thus reducing accuracy and causing patient discomfort. A possible solution is the use of optical 3-D coordinate measuring techniques, such as the fringe projection technique, which enables precise and contact-free recording of facial surfaces. The application of this method is demonstrated on a patient who lost his left eye and part of the jaw bone during surgery for cancer. 3-D CAD software that supports the construction of a facial prosthesis from the data obtained has been developed. For this purpose, spline functions are used to define border curves, and the intact half of the face is used as a model for the prosthetic surface. The resulting digital data are used to construct first a model made of synthetic resin, and then a final wax model with the aid of rapid prototyping techniques.

Computer-Aided Design↗

Gastric wall elasticity assessed by dynamic holographic endoscopy: ex vivo investigations in the porcine stomach.

BACKGROUND: Holographic interferometry is based on the superimposition of the holograms of different motional states of an object on a single holographic storage medium. It has been used to detect structural changes in prosthetic heart valves. The combination of holographic interferometry and endoscopic imaging were applied to assess disturbances of porcine stomach wall elasticity. METHODS: By connecting an electronic speckle pattern interferometry (ESPI) camera system (light source: continuous wave argon-ion laser, lambda = 514.5 nm) to different types of endoscopes, ex vivo experiments were performed on porcine stomachs to detect areas characterized by altered tissue elasticity. With linkage of the endoscopic ESPI camera complex to a fast image processing system, the method of double pulse exposure image subtraction was applied at a video frame rate of 12.5 Hz. RESULTS: The speckle correlation patterns resulting from gentle gastric wall deformation were analyzed in a series of experiments in 16 porcine stomachs. Interferograms of gastric wall areas without structural abnormalities exhibited concentric fringes, whereas fringe patterns corresponding to areas of reduced tissue elasticity were characterized by parallel lines. CONCLUSION: Applying the nondestructive method of dynamic holographic endoscopy, abnormalities of the gastric wall leading to diminished tissue elasticity can be distinguished reliably from surrounding healthy tissue.

Animals↗

Three-dimensional quantification of color-marked occlusal paths on anatomically oriented casts.

STATEMENT OF PROBLEM: The analysis of occlusal contacts on mounted diagnostic casts is an important task in prosthetic diagnostics. However, it is still restricted mainly to qualitative aspects because existing measuring techniques fail to provide 3-dimensional data for more than a few single points in acceptable time. PURPOSE: The aim of this study was to develop a method for quantifying occlusal contacts and paths by using anatomically oriented diagnostic casts and to design the method to allow fast acquisition of digital 3-dimensional coordinates. MATERIAL AND METHODS: Plaster casts with color-marked contacts were digitized optically with a profilometric system based on the fringe projection technique. Digital camera images taken simultaneously were used to define the contact areas by marking them either manually or automatically with the help of image-processing routines. Three-dimensional coordinates were determined by finding the corresponding points within the profilometric data set. RESULTS: Color-marked contact areas on diagnostic casts were successfully digitized with a computer-controlled, automatic setup in approximately 30 seconds. The accuracy of the acquired 3-dimensional data was estimated to be better than 60 microm in lateral and 30 microm in height resolution. The data set was visualized and evaluated in a skull related coordinate system. SUMMARY: This study verified the use of a new tool to quantify color-marked occlusal contacts on diagnostic casts in terms of spatial coordinates. The resulting digital data may be stored easily and analyzed numerically as well as visualized 3-dimensionally with computer graphic equipment. Because the anatomic orientation of the casts is maintained throughout the measurement process, it is possible to compare the data with electronically registered condyle paths and therefore to investigate, for example, their relation to the corresponding guidance paths of the frontal teeth and the canines in dynamic occlusion.

Color↗

Three-dimensional acquisition and visualization of dental arch features from optically digitized models.

A method for the acquisition and evaluation of 3D coordinates from anatomically oriented plaster casts is presented, which is based on optical phase shifting profilometry (a fringe projection technique). With the computer-controlled setup, measurements from different views can be combined to obtain a complete three dimensional reconstruction of the model surface. To allow faster evaluation, the result is converted into a range image. From this digital data set the characteristic features like cusp tips can be identified and located semi-automatically. Based on these marks, quantitative values for differences between situation models like local displacements, e.g. during orthodontic treatment, can be determined. The results are visualized as interactively controllable 3D computer graphics, which helps to make spatial relations clearer.

Calibration↗

Three-dimensional visualization and quantification of the mandibular articular surface by optical profilometry.

The aim of this study was to introduce a nondestructive optical technique for the quantitative assessment of natural biological surfaces as demonstrated with the example of the articular surface of the human temporomandibular joint. The computer-assisted quantitative evaluation of the surface is realized by the acquisition of three-dimensional images via the optical technique of phase measuring profilometry. After mathematical processing of the data set the resulting image can be visualized as three-dimensional object surface reconstructions or as grid surfaces from which arbitrary sections may easily be extracted. From such single sections we can calculate a value that represents the degree of height deviations of the section profile and can be regarded as a parameter for the surface roughness. Further quantitative information about the surface topography is provided by the Fourier transform analysis of the profiles. The Fourier spectrum contains information about the spatial distribution of roughness-causing protuberances along the overall surface. As an example one healthy condyle and one remodeled condyle from macerated cadaver mandibles were investigated. For the two samples evaluated we calculated a mean surface roughness of the entire articular surface with a value of 0.03 +/- 0.005 mm for the healthy condyle with its smooth surface and a value of 0.14 +/- 0.009 mm for the remodeled condyle. We recommend optical profilometry as a sophisticated technique for a more objective and quantitative pathological classification of articular surfaces and similar objects.

Fourier Analysis↗

In vitro testing of bioprostheses: influence of mechanical stresses and lipids on calcification.

BACKGROUND: Structural valve deterioration of bioprostheses is mainly caused by the progressive development of calcification. Mechanical stresses or lipid deposits in porcine aortic leaflets have been proposed as major factors contributing to the calcification process. METHODS: A new test protocol consisting of nondestructive holographic interferometry, which allows a quantitative deformation analysis of heart valves, and accelerated dynamic in vitro calcification was used. The rapid calcification fluid contained a final combined calcium and phosphorus concentration of 130 (mg/dL)2 in barbital buffer solution. The calcification of 32 bioprostheses donated by different manufacturers (SJM Bioimplant, Biocor standard, Biocor No-React, Carpentier-Edwards SAV, Bravo, pericardial prototype) was assessed after up to 25 x 10(6) cycles by microradiography and the areas of calcification were compared with the holographic interferograms. The distribution of lipid droplets of four porcine prostheses were visualized by Sudan III stain before the calcification process. RESULTS: Most of the tested bioprostheses had areas presenting with stress concentrations, and the dynamic in vitro testing resulted in leaflet calcification corresponding to the holographic irregularities. A strong correlation between calcification and stress distribution or lipid accumulation was found (r = 0.72; r = 0.81, respectively). After 19 x 10(6) cycles, the Carpentier-Edwards SAV and the pericardial valves had significantly less calcification than other prostheses tested (p = 0.003), but the variation among individual prostheses from the same manufacturer was even more pronounced. CONCLUSIONS: Mechanical stresses or lipid accumulation seems to play an important role in the calcification process of bioprostheses. Quality control of bioprosthetic valves using holographic interferometry has the potential to predict calcification before implantation.

Aortic Valve↗

Holographic interferometry and in vitro calcification: comparing pericardial versus porcine bioprostheses.

BACKGROUND AND AIMS OF THE STUDY: Structural valve deterioration of bioprostheses is mainly caused by progressive calcification. It has not yet been convincingly demonstrated whether pericardial or porcine bioprostheses are more prone to calcification. METHODS: A previously described in vitro test protocol consisting of non-destructive holographic interferometry, which permits quantitative deformation analysis of heart valves and accelerated dynamic calcification in vitro was used to evaluate five stented pericardial bioprostheses of different sizes and design (three or two leaflets) from one manufacturer. The extent of calcification was assessed after up to 20 x 10(6) cycles in the valve tester by microradiography, and areas of calcification were compared by holographic interferometry using computerized image processing. Calcification was confirmed by EDX-analysis and Von Kossa staining. Results were compared with in vitro testing of 25 porcine bioprostheses from different manufacturers. RESULTS: The tested pericardial bioprostheses had an individual distribution of mechanical stresses detectable by holographic interferometry, which resulted in different calcification of valve leaflets. A strong correlation between calcification and stress distribution was found (correspondence of affected areas: 82.3 +/- 10.1%, r = 0.97). Variability in calcification and stress distribution, respectively, of pericardial valves compared well with our findings for porcine prostheses. Overall, the extent of leaflet calcification was not statistically different for pericardial and porcine bioprostheses (p = 0.21). CONCLUSIONS: The biological material of bioprostheses (pericardial versus porcine) does not seem to be the crucial factor in the calcification process. Mechanical stresses detectable by holographic interferometry have a more pronounced impact and predict calcification of individual prostheses, at least in the in vitro setting.

Animals↗

Quality control of bioprosthetic heart valves by means of holographic interferometry.

BACKGROUND AND AIMS OF THE STUDY: Limited durability of porcine bioprostheses is mainly caused by the progressive development of calcification. We tested the hypothesis that hidden tissue anomalies or unfavorable stress concentrations of commercially available bioprostheses may lead to later calcification and dysfunction. Application of holographic interferometry for non-destructive testing of biological heart valves enables a full-field analysis of heart valves and reveals deformation irregularities of valve tissue. MATERIAL AND METHODS: We developed an accelerated calcification protocol for bioprosthetic heart valves including an accelerated pulsatile valve tester for simultaneous testing of 10 heart valves under identical conditions and a rapid synthetic calcification fluid containing a final Ca x P of 130 (mg/dl)2 in barbital buffer solution. Ten porcine bioprostheses (St. Jude Medical, Bioimplant) were assessed by holographic interferometry and subjected to the pulsatile accelerated calcification process. Distribution and amount of calcification was evaluated by microradiography after 12 x 10(6) and 19 x 10(6) cycles, respectively. Areas of irregular fringe patterns detected by holography as well as areas of calcification were calculated and compared using a personal computer. RESULTS: All tested bioprostheses had localized or extended areas with holographic irregularities and the accelerated valve testing protocol resulted in even macroscopically visible calcifications at various sites. Comparative analysis of the obtained microradiographs revealed that 74.2% +/- 6.0% of calcified leaflet areas lay within the previously detected holographic anomalies. CONCLUSIONS: Our first results show a strong correlation between holographic anomalies and calcification of porcine bioprostheses. We conclude that suitable methods for evaluation and quality control of bioprosthetic heart valves are available and seem to be predictive with regard to valve calcification.

Aortic Valve↗

Measurement of elastic modulus of the central bovine cornea by means of holographic interferometry. Part II. Results.

BACKGROUND: The structural and elastic properties of the cornea play an important role in the outcome of corneal refractive surgery. A way to describe the elastic properties is to calculate Young's modulus. The tangent Young's modulus (Eit) represents the first part of the viscoelastic response of the intact cornea due to intraocular pressure increase. METHOD: The tangent Young's modulus (Eit) of the central cornea of 16 fresh enucleated intact bovine eyes determined from the immediate response was calculated by means of holographic interferometry. The stress was an increase of the intraocular pressure. In 16 eyes the change of intraocular pressure was 10 Pa (group I), starting with an intraocular pressure of 1340 Pa (about 10 mm Hg). In 6 of these 16 eyes there was a second pressure increase of 20 Pa starting at the same intraocular pressure of 1340 Pa (about 10 mm Hg) (group II). RESULTS: The mean value for the Young's modulus in group I was 1.58 x 10(5) Pa. For group II the Young's modulus was 1.60 x 10(5) Pa. The difference was not significant. The values for the Young's moduli of the cornea of intact eyes are about 100 times smaller than calculations based on experiments with strips of the cornea. CONCLUSION: It is necessary to consider the localization of the corneal tissue to avoid influences of corneal hydration or dehydration and to define the existing intraocular pressure if Young's modulus of the cornea is to be calculated.

Animals↗

Comparative analysis of glutaraldehyde-preserved porcine xenografts and fresh or glutaraldehyde-treated human aortic valves by holographic interferometry.

Although calcification and degeneration are recognized as the main causes of bioprosthetic heart valve failure, the reasons for such failure are not well understood. Hidden tissue anomalies in the valves may be the origin of later calcification. Application of hologram interferometry for non-destructive testing enables the detection of such tissue anomalies. A comparative study by holographic interferometry of ten porcine bioprosthetic valves (seven Carpentier-Edwards SAV, two BioImplant and one Valcor) with five human aortic valves before and after glutaraldehyde treatment is presented. Whereas irregularities were detected in the interferograms of eight out of ten bioprostheses, no similar distorted fringe pattern was found in the holographic interferograms of human specimens. The present results suggest that tissue abnormalities exist in standard bioprosthetic valves which are absent in human ones. These irregularities may be the origin of later calcification and valvular dysfunction.

Aged↗

Holographic interferometry: a new technique for in vitro investigations of prosthetic heart valves.

At present there are many different prosthetic models available for heart valve replacement. Postimplant dysfunction resulting from material failure has been reported in several prostheses. The prime cause of these defects is hidden abnormalities in the valve construction or materials. In order to detect these defects before implantation, preoperative non-destructive testing of individual valves is proposed. For this purpose, holographic interferometry has been applied which is a non-contact, non-destructive, highly sensitive, three-dimensional measurement technique. Samples of different types of prosthetic heart valve, both mechanical and biological, were mounted in a specially developed test chamber with optical access from four sides. The valves were loaded with a static liquid pressure of 2.5-15 kPa. Deformations of the valves as a result of small pressure differences (0.5-15% of the static pressure) applied between two exposures were recorded by double-exposure holography. A fringe pattern superimposed on the image of the valve reconstructed from the hologram clearly indicates the presence of defects in the valve material.

Heart Valve Prosthesis↗

Biomechanical investigation of the hyoid bone using speckle interferometry.

Speckle interferometry is a non-destructive measurement technique using a laser. The principles of the method are described. Human hyoid bones (n = 10) were investigated. Loads were applied to individual points on the anterior surface of the body of hyoid bones. All hyoid bones showed an asymmetric displacement. The displacement was greatest in a plane vertical to the load. An evaluation of fracture behavior is possible due to the displacement pattern.

Adult↗

Measurement of elastic modulus of the bovine cornea by means of holographic interferometry. Part 1. Method and experiment.

Holographic interferometry of the cornea of fresh, enucleated bovine eyes placed in air was used for determination of the elastic modulus of the central part of the cornea. The high sensitivity of the method enables measurement of the radial distension of the cornea even for intraocular pressure (IOP) changes equal to 5 Pa (0.05 mbar or approximately 0.0375 mm Hg). The method of calculation of the tangent Young's modulus of the cornea Et, based on data obtained from double exposure as well as real-time holographic interferograms and on some geometrical data describing the form of the cornea are presented. The average value of Et amounting to 1.42.10(5) Pa was calculated from 2 interferograms of the same eye.

Animals↗

Non-destructive evaluation of prosthetic heart valves by holographic interferometry.

Dysfunction of prosthetic heart valves is a common complication after heart valve replacement, affecting both biologic and mechanical prostheses. A preoperative, non-destructive test of each individual valve may help to prevent the implantation of a valve which has material weaknesses. To this end we developed a technique for testing heart valve prostheses by holographic interferometry. The advantage of this technique is that it provides a non-contact, non-destructive, highly sensitive three dimensional analysis of the valve under loading. Samples of several mechanical and biologic valve substitutes were investigated. Deformations of the valve, due to small pressure differences applied to the samples in a specially developed test chamber, were recorded by double exposure holography. A fringe pattern superimposed on the image of the valve reconstructed from the hologram clearly indicates the presence of even the slightest defect in the valve material. Our experimental results demonstrate the ability of non-destructive holographic screening testing to detect defects or weaknesses which may potentially lead to dysfunction in replacement valves.

Animals↗

Non-destructive evaluation techniques for prosthetic heart valves based on hologram interferometry. Part I.

The development of a technique applying hologram interferometry, which is a non-destructive, non-contact, full-field, highly sensitive method is reported. The valve under investigation is placed in a test chamber which has windows providing optical access. Deformations of the valve leaflets due to pressure loading are recorded by hologram interferometry. The resulting interferogram clearly indicates the existence of any defects or structural anomalies which may be present in the valve material. Three modifications to this technique, intended for qualitative and quantitative non-destructive valve screening tests are described. The proposed technique is expected to become an effective means of detecting hidden defects of replacement heart valves; it is thus considered as a prospective tool for quality control, particularly in the manufacture of bioprosthetic valves, where initial sites of late calcification and degeneration might be identified. The application of holographic non-destructive testing may therefore substantially improve the quality and durability of heart valve substitutes.

Artifacts↗

Non-destructive evaluation techniques for prosthetic heart valves based on hologram interferometry. Part II: Experimental results and clinical implications.

Non-destructive evaluation by hologram interferometry of seven mechanical and seven bioprosthetic valves was carried out. Irregular fringe patterns suspect of intrinsic valve anomaly which may lead to later dysfunction were detected in one mechanical and six bioprosthetic valves. Histologic examination of two bioprosthetic valves revealed focal degeneration, especially in the fibrosa, in those parts of the leaflets that had obvious anomalies on the holographic interferograms. It was shown that the flow turbulences caused by prosthetic valves can also be evaluated using hologram interferometry. The experimental results, obtained with six different types of prostheses (Lillehei-Kaster, Bjork-Shiley, Omnicarbon, St. Jude Medical, Carpentier-Edwards S.A.V. and Valcor), demonstrated the applicability of this technique to both mechanical and bioprosthetic valves. Carrying out non-destructive screening tests for heart valve prostheses may prevent the implantation of potentially dysfunctional devices.

Animals↗

[Qualitative analysis of the elasticity of the bovine cornea by holographic interferometry].

Double-exposure holographic interferometry is used for deformation analysis of the surface of bovine corneas subjected to intraocular pressure increase. Reproducible individual fringe patterns are recorded for an intraocular pressure of 1340 Pa and subsequent pressure changes of 20, 40 and 80 Pa, respectively. These interference fringes characterize loci of equal displacement. In spite of interindividual variations of the central fringe pattern some basic types can be differentiated: round, oval, drop-like, double-centers, symmetrical as well as unsymmetrical and irregular forms. The peripheral fringe patterns of the cornea can be described by 3 basic types, even taking into account individual variations: circular, circular with an irregular component and irregular. The precision of the method allows to visualize minute local elasticity differences. Results of these investigations do not support the assumption, that the center of the cornea has a higher elasticity than peripheral regions in general. These elasticity differences between central and peripheral areas vary between individual corneas for small intraocular pressure changes at a basic pressure of ca. 10 mm Hg (1340 Pa).

Animals↗