Biomedical subjects

Gábor Horváth

Publications and source records attributed to Gábor Horváth.

At least 19 recordsLinked to original sources

PubMed2007

Polarization of 'water-skies' above arctic open waters: how polynyas in the ice-cover can be visually detected from a distance.

The foggy sky above a white ice-cover and a dark water surface (permanent polynya or temporary lead) is white and dark gray, phenomena called the 'ice-sky' and the 'water-sky,' respectively. Captains of icebreaker ships used to search for not-directly-visible open waters remotely on the basis of the water sky. Animals depending on open waters in the Arctic region may also detect not-directly-visible waters from a distance by means of the water sky. Since the polarization of ice-skies and water-skies has not, to our knowledge, been studied before, we measured the polarization patterns of water-skies above polynyas in the arctic ice-cover during the Beringia 2005 Swedish polar research expedition to the North Pole region. We show that there are statistically significant differences in the angle of polarization between the water-sky and the ice-sky. This polarization phenomenon could help biological and man-made sensors to detect open waters not directly visible from a distance. However, the threshold of polarization-based detection would be rather low, because the degree of linear polarization of light radiated by water-skies and ice-skies is not higher than 10%.

Algorithms↗

PubMed2006

Positive polarotaxis in a mayfly that never leaves the water surface: polarotactic water detection in Palingenia longicauda (Ephemeroptera).

Tisza mayflies, Palingenia longicauda (Olivier 1791), swarm exclusively over the river Tisza (from which the name of the mayfly was derived). This river is bordered by a high vertical wall of trees and bushes, which hinder P. longicauda to move away horizontally from the water. During swarming, Tisza mayflies fly immediately above the river in such a way that their cerci touch the water frequently or sweep its surface. This continuous close connection with water and the vertical wall of the shore and riparian vegetation result in that Tisza mayflies never leave the water surface; consequently, they need not search for water. Several Ephemeroptera species move away far from water and return to it guided by the horizontal polarization of water-reflected light. To reveal whether also P. longicauda is or is not polarotactic, we performed a field experiment during the very short swarming period of Tisza mayflies. We show here that also P. longicauda has positive polarotaxis, which, however, can be observed only under unnatural conditions, when the animals are displaced from the water and then released above artificial test surfaces. P. longicauda is the first species in which polarotactic water detection is demonstrated albeit it never leaves the water surface, and thus, a polarotactic water detection seems unnecessary for it. The polarotactic behaviour of Tisza mayflies explains the earlier observation that these insects swarm above wet asphalt roads running next to river Tisza.

Animals↗

PubMed2006

Why do highly polarizing black burnt-up stubble-fields not attract aquatic insects? An exception proving the rule.

Horizontal black surfaces are usually very attractive to polarotactic aquatic insects, since they generally reflect highly and horizontally polarized light, and thus imitate water surfaces. We monitored highly polarizing black burnt-up stubble-fields, but surprisingly never found aquatic insects or their carcasses in the ash, although flying polarotactic insects were abundant in the area, which we showed by attracting them to horizontal black plastic sheets. To explain this observation, we measured the reflection-polarization characteristics of burnt-up stubble-fields in the red (650 nm), green (550 nm) and blue (450 nm) parts of the spectrum at three directions of view relative to the solar meridian. We established that (i) the degree of linear polarization p of light reflected from the black ash is high; (ii) p is the higher, the darker the ash; (iii) the direction of polarization of reflected light is nearly horizontal only towards the solar and antisolar meridians, and it is tilted in other directions of view; (iv) the standard deviation of both the degree and the direction of polarization of reflected light is large. The latter two characteristics explain why burnt-up stubble-fields are unattractive to aquatic insects. These results may be important in the study of the wider environmental effects of post-harvest burning.

Animals↗

PubMed2006

Why do red and dark-coloured cars lure aquatic insects? The attraction of water insects to car paintwork explained by reflection-polarization signals.

We reveal here the visual ecological reasons for the phenomenon that aquatic insects often land on red, black and dark-coloured cars. Monitoring the numbers of aquatic beetles and bugs attracted to shiny black, white, red and yellow horizontal plastic sheets, we found that red and black reflectors are equally highly attractive to water insects, while yellow and white reflectors are unattractive. The reflection-polarization patterns of black, white, red and yellow cars were measured in the red, green and blue parts of the spectrum. In the blue and green, the degree of linear polarization p of light reflected from red and black cars is high and the direction of polarization of light reflected from red and black car roofs, bonnets and boots is nearly horizontal. Thus, the horizontal surfaces of red and black cars are highly attractive to red-blind polarotactic water insects. The p of light reflected from the horizontal surfaces of yellow and white cars is low and its direction of polarization is usually not horizontal. Consequently, yellow and white cars are unattractive to polarotactic water insects. The visual deception of aquatic insects by cars can be explained solely by the reflection-polarizational characteristics of the car paintwork.

Animals↗

PubMed2006

[Haemochromatosis].

Explore the source record for details and available documents.

Diagnosis, Differential↗

PubMed2006

Imaging polarimetry of the circularly polarizing cuticle of scarab beetles (Coleoptera: Rutelidae, Cetoniidae).

The light reflected from the metallic-shiny regions of the cuticle of certain beetles belonging to the Scarabaeoidea is known since 1911 to be left-handed circularly polarized. Only photographs of a few selected species of scarabs, taken through left- and right-circular polarizers, have earlier been published. Through a right-circular polarizer these beetles appear more or less dark. This demonstration is, however, inadequate to quantitatively investigate the spatial distribution and the wavelength dependency of the circular polarization of light reflected from the scarab cuticle. In order to overcome this problem, we have developed a portable, rotating analyzer, linear/circular, digital, and imaging polarimeter. We describe here our polarimetric technique and present for the first time the linear and circular polarization patterns of the scarab species Chrysophora chrysochlora, Plusiotis resplendens (Rutelidae), and Cetonischema jousselini (Cetoniidae) in the red (650 nm), green (550 nm), and blue (450 nm) parts of the spectrum. We found the wavelength- and species-dependent circular polarization patterns in scarabs to be of a rather complex nature. These patterns are worthy of further studies.

Animals↗

PubMed2006

[Prevalence of musculoskeletal symptoms in adulthood and adolescence (survey conducted in the Southern Transdanubian region in a representative sample of 10.000 people].

UNLABELLED: The time period between 2000-2010 has been declared the "bone and joint decade" by the World Health Organisation. Throughout this time the international scientific interest focuses mainly on the muskuloskeletal diseases: epidemiology, preventive and therapeutic measures. OBJECTIVE: Joining this international effort authors aim was to define the prevalence of the musculoskeletal symptoms and the general musculoskeletal state of health of a representative sample in the hungarian population. METHOD: Obtaining data from the Central Office of Statistics, a sample of 10 thousand people living in the South Transdanubian Region aged between 14-65 years was selected. The sample was representative concerning age, employment and municipal structure. A survey was performed by asking the people to fill out a questionnaire made by the authors, which consisted of questions focusing on musculoskeletal symptoms, and the Short Form-36 questionnaire. RESULTS: Based on questionnaire data, approximate prevalence values of the different musculoskeletal complaints were obtained. Thus, the prevalence of low back pain was 44.1%, hip pain occurred in 22.2%, knee pain in 30.3%, and ankle pain in 9.7% in the observed population. CONCLUSION: Compared with the international data, authors conclude that the prevalence of the musculoskeletal symptoms: low back pain, hip and knee pain is superior in the observed sample. Based on the results of the Short Form-36 questionnaire it seems that in the majority of the examined parameters the state of health is worse in the Southern Transdanubian population compared to the Western European and the North American Populations.

Adolescent↗

PubMed2006

Experimental and theoretical study of skylight polarization transmitted through Snell's window of a flat water surface.

The celestial polarization pattern may be scrambled by refraction at the air-water interface. This polarization pattern was examined in shallow waters with a submersible polarimeter, and it was calculated by using land measurements ('semiempirical predictions') and models of the skylight polarization. Semiempirically predicted and measured e-vector orientations were significantly similar. Conversely, predicted percent polarization was correlated but lower than measurements. Percent polarization depended on wavelength, where at high sun altitudes maximal percent polarization generally appeared in the UV and red spectral regions. The wavelength dependency of polarization may lead to differential spectral sensitivity in polarization-sensitive animals according to time and type of activity.

Computer Simulation↗

PubMed2006

Detection of gentamicin emission from bone cement in the early postoperative period following total hip arthroplasty.

This article describes the characteristics of gentamicin emission from the bone cement-antibiotic complex in the early postoperative period following total hip arthroplasty. Gentamicin levels of the drain fluid taken at 6, 24, and 48 hours postoperatively were measured with a fluorescent polarization immunoassay method. Mean gentamicin concentrations were 2.6, 1.2, and 0.6 mcg/mL, respectively. Age, sex, and body mass index had no significant influence on the outcome. Results showed that the amount of gentamicin in the wound fluid is inversely proportional to the total amount excreted. Twenty-four hours postoperatively, the average gentamicin concentrations in the drain fluid taken from around the endoprosthesis implanted with Palacos-R bone cement (Zimmer Warsaw, Ind), diminished, yet remained above the minimal inhibitory concentration level.

Aged↗

PubMed2005

Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy.

The anaphase-promoting complex (APC), a multisubunit E3 ubiquitin ligase, is an essential regulator of the cell cycle from metaphase until S phase in yeast and metazoans. APC mediates degradation of numerous cell cycle-related proteins, including mitotic cyclins and its activation and substrate-specificity are determined by two adaptor proteins, Cdc20 and Cdh1. Plants have multiple APC activators and the Cdh1-type proteins, in addition, are represented by two subclasses, known as Ccs52A and Ccs52B. The Arabidopsis genome contains five cdc20 genes as well as ccs52A1, ccs52A2 and ccs52B. In Schizosaccharomyces pombe, expression of the three Atccs52 genes elicited distinct phenotypes supporting nonredundant function of the AtCcs52 proteins. Consistent with these activities, the AtCcs52 proteins were able to bind both to the yeast and the Arabidopsis APCs. In synchronized Arabidopsis cell cultures the cdc20 transcripts were present from early G2 until the M-phase exit, ccs52B from G2/M to M while ccs52A1 and ccs52A2 were from late M until early G2, suggesting consecutive action of these APC activators in the plant cell cycle. The AtCcs52 proteins interacted with different subsets of mitotic cyclins, in accordance with their expression profiles, either in free- or CDK-bound forms. Expression of most APC subunits was constitutive, whereas cdc27a and cdc27b, corresponding to two forms of apc3, and ubc19 and ubc20 encoding E2-C type ubiquitin-conjugating enzymes displayed differences in their cell cycle regulation. These data indicate the existence of numerous APC(Cdc20/Ccs52/Cdc27) forms in Arabidopsis, which in conjunction with different E2 enzymes might have distinct or complementary functions at distinct stages of the cell cycle.

Anaphase-Promoting Complex-Cyclosome↗

PubMed2005

Wall thickness of gas- and marrow-filled avian long bones: measurements on humeri, femora and tibiotarsi in crows (Corvus corone cornix) and magpies (Pica pica).

We studied how the ratio K of the internal to external diameter of gas- and marrow-filled avian long bones follows the biomechanical optima derived for tubular bones with minimum mass designed to fulfil various mechanical requirements. We evaluated radiographs of numerous humeri, femora and tibiotarsi in Corvus corone cornix and Pica pica. The K-values of the gas-filled humerus (K=0.78+/-0.03) and the marrow-filled femur (K=0.79+/-0.02) in Corvus are practically the same, while K of the marrow-filled tibiotarsus (K=0.71+/-0.04) is significantly smaller. The same is true for the gas-filled humerus (K=0.78+/-0.02) and the marrow-filled femur (K=0.77+/-0.02) and tibiotarsus (K=0.67+/-0.05) in Pica. K in Corvus is slightly larger than K in Pica, but the differences are statistically not significant. The standard deviation DeltaK of the tibiotarsi (DeltaK=0.04-0.05) is approximately two times as large as that of the humeri (DeltaK=0.02-0.03) and femora (DeltaK=0.02) in both species. Accepting the assumption of earlier authors that the ratio Q of the marrow to bone density is 0.5, our data show that the marrow-filled tibiotarsi of Corvus and Pica are optimized for stiffness, while the marrow-filled femora are far from any optimum. The relative wall thickness W=1-K of the gas-filled avian humeri studied is much larger than the theoretical optimum W*=1-K*=0.07, and thus these bones are thicker-walled than the optimal gas-filled tubular bone with minimum mass.

Animals↗

PubMed2005

Why do dusk-active cockchafers detect polarization in the green? The polarization vision in Melolontha melolontha is tuned to the high polarized intensity of downwelling light under canopies during sunset.

In the retina of dusk-active European cockchafers, Melolontha melolontha, the linear polarization of downwelling light (skylight or light from the tree canopy) is detected by photoreceptors in upward-pointing ommatidia with maximal sensitivity at 520 nm in the green portion of the spectrum. To date no attempt has been made to answer the question of why these beetles detect polarization in the green. Here we present an atmospheric optical and receptor-physiological explanation of why longer wavelengths are advantageous for the perception of polarization of downwelling light under canopies illuminated by the setting sun. Our explanation focuses on illumination situations during sunset in canopied optical environments, because cockchafers are active at sunset and fly predominantly under canopies during their swarming, feeding, and mating periods. Using three simple atmospheric optical models, we computed the degree of linear polarization, the linearly polarized intensity of downwelling light, the quantum catch, and quantum catch difference between polarization detectors with orthogonal microvilli under canopies illuminated by the setting sun as functions of wavelength and solar zenith angle. Based upon these computations, we show that the green sensitivity of polarization detectors in M. melolontha is tuned to the high polarized intensity of downwelling light in the green under canopies during sunset, an optimal compromise between simultaneous maximization of the quantum catch and the quantum catch difference. We also briefly discuss how green-sensitive polarization detectors can function efficiently enough during the pre-feeding and egg-laying flights of cockchafers, which always occur prior to sunset and under the sky.

Animals↗

PubMed2005

Epidermal growth factor receptor coexpression modulates susceptibility to Herceptin in HER2/neu overexpressing breast cancer cells via specific erbB-receptor interaction and activation.

BACKGROUND: Growth factors and Herceptin specifically and differentially modulate cell proliferation of tumor cells. However, the mechanism of action on erbB-receptor level is incompletely understood. We evaluated Herceptin's capacity to modulate erbB-receptor activation and interaction on the cell surface level and thereby potentially impair cell proliferation of HER2/neu (c-erbB2) overexpressing breast cancer cells, both in the presence and absence of relevant growth factors. METHODS: BT474 and SK-BR-3 breast cancer cell lines were treated with Epidermal Growth Factor (EGF), Heregulin, and with Herceptin in different combinations. Kinetics of cell proliferation were evaluated flow cytometrically based on BrdU-labeling. Fluorescence Resonance Energy Transfer, ELISAs and phosphorylation site specific Western Blotting was performed to investigate erbB-receptor interaction and activation. RESULTS: EGF induced EGFR/EGFR and EGFR/c-erbB2 interactions correlate with stimulation of cell proliferation in BT474 cells. Both homo- and heterodimerization are considerably less pronounced in SK-BR-3 cells and heterointeraction is additionally reduced by EGF treatment, causing inhibition of cell proliferation. Heregulin stimulates cell proliferation extensively in both cell lines. Herceptin drives BT474 cells more efficiently into quiescence than it does with SK-BR-3 cells and thereby blocks cell cycle progress. In SK-BR-3 Herceptin treatment causes c-erbB2 phosphorylation of Y877 and Y1248, EGF induces Y877 and Y1112 phosphorylation. The Y1112 phosphorylation site, activated by EGF in SK-BR-3 cell, is bypassed in BT474. In addition the inhibitory capacity of Herceptin on BT474 and SK-BR-3 cell proliferation depends on the presence and absence of growth factors to a various extent. CONCLUSION: The growth inhibitory effect of Herceptin on c-erbB2 overexpressing breast cancer cells is considerably modulated by EGFR coexpression and consequently EGFR/c-erbB2 homo- and heterointeractions, as well as the presence or absence of growth factors. C-erbB2 overexpression alone is insufficient to predict the impact of growth factors and antibodies on cell proliferation. The optimization and specification of therapeutic approaches based on erbB-receptor targeting requires to account for EGFR coexpression as well as the potential presence of erbB-receptor relevant growth factors.

Antibodies, Monoclonal↗

PubMed2005

[Dual tumours in the GI tract: synchronous and metachronous stromal (GIST) and epithelial/neuroendocrine neoplasms].

Gastrointestinal stromal tumours (GIST) constitute the most frequent group of mesenchymal tumours in the gastrointestinal tract (GI). During the last several decades major advances have been taken in the diagnostics, treatment, and understanding of its pathogenesis. However, much less is known about the either metachronous or synchronous concurrence of GIST and other tumours of different histogenesis. In the present study clinicopathological data of 43 patients with histologically proved gastrointestinal stromal tumour were studied mainly in regard of the occurrence of a secondary neoplasm. Among the 43, 7 cases were found with secondary tumour mainly of epithelial origin. In three cases (cases 3, 5, and 7) GIST concurred with colorectal adenocarcinoma, in one case (case 1) GIST occurred in a patient with a 3-years-history of chronic lymphocytic leukaemia (CLL), in other two (cases 2 and 4) the stromal tumour was combined with in situ adenocarcinoma of the stomach and carcinoid of the pancreas, respectively. In case 6, GIST concurred with a duodenal Brunner gland adenoma. In five cases the stromal tumour and the other neoplasm occurred synchronously, and in four of them, being the stromal tumour clinically silent, GISTs were intraoperative findings. This confirms the importance of surgical intraabdominal control before closure. In one hand the repeated concurrence of GIST and colorectal adenocarcinoma in our series, and on the other hand, that of GIST and adenocarcinoma of the stomach in the literature, may indicate an at least partly common factor, which may be involved in the pathogenesis of these neoplasms.

Adenocarcinoma↗

PubMed2005

Selecting the right fluorophores and flow cytometer for fluorescence resonance energy transfer measurements.

BACKGROUND: Fluorescence resonance energy transfer applied in flow cytometry (FCET) is an excellent tool for determining supramolecular organization of biomolecules at the cell surface or inside the cell. Availability of new fluorophores and cytometers requires the establishment of fluorophore dye pairs most suitable for FCET measurements. METHODS: A gastric tumor cell line (N87) was labeled for major histocompatibility complex class I heavy chain and beta2-microglobulin with antibodies conjugated with fluorescein- and indocarbocyanine-like fluorophores and analyzed in FCET measurements on a cell-by-cell basis using three flow cytometers: FACSCalibur, FACSDiVa, and FACSArray. RESULTS: Normalized fluorescence intensity values were measured and normalized energy transfer efficiencies, spectral overlap integrals, and crucial dye- and instrument-dependent parameters were calculated for all matching pairs of seven fluorophores on the three commercial cytometers. The most crucial parameter in determining the applicability of the donor-acceptor pairs was the normalized fluorescence intensity and the least important one was the spectral overlap. CONCLUSIONS: On the basis of available laser lines, the optimal dye pair for all three cytometers is the Alexa546-Alexa647 pair, which produces high energy transfer efficiency values and has the best spectral characteristics with regard to laser excitation, detection of emission, and spectral overlap.

Cell Line, Tumor↗

PubMed2005

Computer program for analyzing donor photobleaching FRET image series.

BACKGROUND: The photobleaching fluorescence resonance energy transfer (pbFRET) technique is a spectroscopic method to measure proximity relations between fluorescently labeled macromolecules using digital imaging microscopy. To calculate the energy transfer values one has to determine the bleaching time constants in pixel-by-pixel fashion from the image series recorded on the donor-only and donor and acceptor double-labeled samples. Because of the large number of pixels and the time-consuming calculations, this procedure should be assisted by powerful image data processing software. There is no commercially available software that is able to fulfill these requirements. METHODS: New evaluation software was developed to analyze pbFRET data for Windows platform in National Instrument LabVIEW 6.1. This development environment contains a mathematical virtual instrument package, in which the Levenberg-Marquardt routine is also included. As a reference experiment, FRET efficiency between the two chains (beta2-microglobulin and heavy chain) of major histocompatibility complex (MHC) class I glycoproteins and FRET between MHC I and MHC II molecules were determined in the plasma membrane of JY, human B lymphoma cells. RESULTS: The bleaching time constants calculated on pixel-by-pixel basis can be displayed as a color-coded map or as a histogram from raw image format. CONCLUSION: In this report we introduce a new version of pbFRET analysis and data processing software that is able to generate a full analysis pattern of donor photobleaching image series under various conditions. .

Algorithms↗

PubMed2005

How does the relative wall thickness of human femora follow the biomechanical optima? An experimental study on mummies.

We studied how the ratio (K) of the internal:external diameter of human femora follows the biomechanical optima derived earlier by other researchers for marrow-filled tubular bones with circular cross section and minimum mass designed to withstand yield and fatigue, or stiffness, or bending fracture, or impact strengths. With evaluation of radiographs of 107 femora from 57 human mummies the values of K were measured. We found that K(posterior)=0.498+/-0.085 for the posterior radiographic view, and K(medial)=0.589+/-0.070 for the medial view with K(min)=0.345 and K(max)=0.783. The theoretical optima for K depend on the ratio (Q) of the marrow:bone density. Accepting the assumption of earlier authors that Q=0.50, our data show that human femora are optimised to withstand bending fracture, or yield and fatigue strengths. There were no sex-, age- and length-specific differences in K, and the means of K of the right and left femora of individuals were statistically not significantly different. The biomechanical optimization for K of human femora is not finely tuned. Compared with fox femora, K of human femora follows the biomechanical optimum to a much lesser extent. Although the relative wall thickness W=1-K of human femora are optimised, the very low relative mass increment due to deviation of K from the optimum and the considerable intraspecific variance of K make it probable that an accurate optimization of the relative wall thickness is irrelevant in humans.

Adolescent↗

PubMed2005

Psychophysical study of the visual sun location in pictures of cloudy and twilight skies inspired by Viking navigation.

In the late 1960s it was hypothesized that Vikings had been able to navigate the open seas, even when the sun was occluded by clouds or below the sea horizon, by using the angle of polarization of skylight. To detect the direction of skylight polarization, they were thought to have made use of birefringent crystals, called "sun-stones," and a large part of the scientific community still firmly believe that Vikings were capable of polarimetric navigation. However, there are some critics who treat the usefulness of skylight polarization for orientation under partly cloudy or twilight conditions with extreme skepticism. One of their counterarguments has been the assumption that solar positions or solar azimuth directions could be estimated quite accurately by the naked eye, even if the sun was behind clouds or below the sea horizon. Thus under partly cloudy or twilight conditions there might have been no serious need for a polarimetric method to determine the position of the sun. The aim of our study was to test quantitatively the validity of this qualitative counterargument. In our psychophysical laboratory experiments, test subjects were confronted with numerous 180 degrees field-of-view color photographs of partly cloudy skies with the sun occluded by clouds or of twilight skies with the sun below the horizon. The task of the subjects was to guess the position or the azimuth direction of the invisible sun with the naked eye. We calculated means and standard deviations of the estimated solar positions and azimuth angles to characterize the accuracy of the visual sun location. Our data do not support the common belief that the invisible sun can be located quite accurately from the celestial brightness and/or color patterns under cloudy or twilight conditions. Although our results underestimate the accuracy of visual sun location by experienced Viking navigators, the mentioned counterargument cannot be taken seriously as a valid criticism of the theory of the alleged polarimetric Viking navigation. Our results, however, do not bear on the polarimetric theory itself.

Computer Graphics↗