Enzyme responsive polymer hydrogel beads.
We report on a new class of enzyme responsive polymer hydrogels, the molecular accessibility of which can be changed selectively by enzymes present in a sample fluid.
Enzymes↗
Biomedical subjects
Gail McConnell
Publications and source records attributed to Gail McConnell.
Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2.
We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
Animals↗
Using two photon microscopy to quantify enzymatic reaction rates on polymer beads.
Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports.
Acrylamides↗
Two-photon microscopy to spatially resolve and quantify fluorophores in single-bead chemistry.
Explore the source record for details and available documents.
Journal Article↗
Two-photon laser scanning fluorescence microscopy using photonic crystal fiber.
We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiation of less than 35 fs duration reaching the sample.
Animals↗