Biomedical subjects

Gang Cheng

Publications and source records attributed to Gang Cheng.

21 records · Page 2Linked to original sources

PubMed2002

Development and clinical trial of fluorescence PCR diagnostic kit to detect Mycobacterium tuberculosis.

OBJECTIVE: To develop a new diagnostic kit for Mycobacterium tuberculosis (TB) detection on the basis of fluorescence PCR (F-PCR) and conduct clinical trial of this kit for assessing its performance in comparison with other diagnostic methods. METHODS: We collected 546 clinical sputum samples from patients with phthisis and normal subjects to examine TB by way of F-PCR in parallel with examination with modified R.culture, auramine smear with fluorescence staining and LCx DNA diagnostic kit from Abbott for comparison. RESULTS: A clinical diagnostic kit for TB detection was developed with F-PCR, and clinical trial showed that its positivity rate was 49.1%, sensitivity 89.2%, specificity 98.8% and efficiency 93.6%. CONCLUSION: F-PCR is obviously superior to culture method and smear method and comparable with LCx in terms of sensitivity, and can be used to monitor TB DNA in the secretions to facilitate clinical diagnose and therapeutic effects monitoring.

DNA, Bacterial↗

PubMed2002

[Study of immunological indicators in peripheral blood for nasopharyngeal carcinoma].

OBJECTIVE: To find reliable diagnostic immunological indicators in peripheral blood for nasopharyngeal carcinoma, and to evaluate the possibility of cancer screening and early diagnosis of the malignancies in the future. METHODS: Plasma samples were obtained from 83 patients with nasopharyngeal carcinoma and from 105 patients with benign diseases. Nine cytokines were selected as the candidates for the indicators and their quantity in the plasma samples determined by enzyme-linked immunosorbent assay, the result of which was analyzed statistically. RESULTS: Interleukin (IL)-4 level was obviously increased, while IL-6, IL-12, transforming growth factor (TGF)-beta, interferon (IFN)-gamma levels significantly reduced (P<0.05) in the plasma from patients with nasopharyngeal carcinoma. CONCLUSION: These cytokins may serve as the indicators in the diagnosis of nasopharyngeal carcinoma.

Biomarkers, Tumor↗

PubMed2002

[Nausea disintegrating buccal tablet in the prevention of gastrointestinal reaction induced by anticancer drugs].

OBJECTIVE: To evaluate the efficacy and safety of nausea oral, disintegrating buccal tablet (DBT) in the prevention of gastrointestinal reaction induced by anticancer drugs (cisplatin DDP 30 - 50 mg/m(2) or adramycin ADM >/= 40 mg/m(2)), as compared with those of kytril tablets. METHODS: A multicenter, open and randomized self-crossover control trial was carried out with all the eligible patients randomized into AB or BA group. Patients in AB group were given nausea 0.1 mg as buccal tablet one hour before chemotherapy in the first cycle and kytril tablet 2 mg in the second cycle, those in BA group were given these drugs in the reverse order. RESULTS: Seventy-three patients were allotted to this study, including 44 patients in DDP-arm and 29 in ADM-arm. Sixty-two patients were evaluable for response and 70 patients for safety. Nausea DBT was as effective as kytril tablet in the control of anorexia, nausea and vomiting during the first 24 hours after chemotherapy, with response rates of 74.2%, 77.4%, 83.9% in nausea DBT and 74.2%, 71.0%, 88.7% DBT in kytril tablets. A high efficacy in the control of vommitting induced by cisplatin was observed in both nausea DBT and kytril tablets. The complete control rates and overall control rates were 83.3%, 91.7% in nausea DBT and 86.1%, 97.2% in kytril tablets, respectively. The side effects of nausea DBT were head heaviness, dry mouth and somnolence, which were mild and comparable with kytril in their frequencies. CONCLUSION: Nausea disintegrating buccal tablet is able to effectively prevent gastrointestinal reaction induced by anticancer drugs, with efficacy and side effects similar to kytril tablets. Nausea DB tablet, an intraoral disintegrator, is very convenient for patients who can not swallow tablets for various reasons.

Adolescent↗