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Gang Su

Publications and source records attributed to Gang Su.

14 recordsLinked to original sources
PubMed2006

Neoadjuvant intratumoral cytokine-loaded microspheres are superior to postoperative autologous cellular vaccines in generating systemic anti-tumor immunity.

BACKGROUND: Sustained intratumoral cytokine release using poly-lactic acid microspheres (PLAMs) can induce a systemic immune response, shifting immunotherapy to the neoadjuvant setting. METHODS: C57BL6 mice with established B16 melanomas underwent a single intralesional injection of IL-12, TNF-alpha or GM-CSF PLAM, alone or in combination. Tumor draining lymph nodes (TDLN) and spleens were assessed for a specific anti-tumor response by IFNgamma release assay and ELISPOT. RESULTS: Intralesional injection of TNF-alpha, alone or in combination, resulted in significant tumor ablation. The induction of tumor specific reactive T-cells in the TDLN was greatest with IL-12 and TNF-alpha. Only mice treated with IL-12 and TNF-alpha demonstrated a substantial T-cell response in cultured splenocytes. This combination resulted in a significant reduction in new tumors after re-challenge. Adjuvant therapy, using irradiated B16 cells in combination with equivalent doses of IL-12 and TNF-alpha, failed to generate a similar T-cell response or prevent re-challenge. CONCLUSIONS: Intratumoral IL-12 and TNF-alpha loaded PLAM leads to both local eradication of tumor and the induction of specific anti-tumor T-cells in the lymph nodes and spleens, resulting in memory immune response. Neoadjuvant treatment was significantly superior to postoperative autologous cellular vaccines using IL-12 and TNF-alpha PLAM.

Animals↗
PubMed2006

Adoptive immunotherapy of breast cancer with lymph node cells primed by cryoablation of the primary tumor.

Cryoablation of cancer leaves tumor-associated antigens intact in an inflammatory microenvironment that can stimulate a regional anti-tumor immune response. We examined whether cryoablated tumor draining lymph nodes (CTDLN) as adoptive immunotherapy may be an effective immunotherapeutic approach in the adjuvant treatment of breast cancer. BALB/c mice with MT-901 mammary adenocarcinoma tumors underwent cryoablation, resection or no treatment and tumor draining lymph nodes were harvested. Cryoablation resulted in only a mild increase in the absolute number of T-cells but a significant increase in the fraction of tumor-specific T-cells as evidenced on IFN-gamma release assay. FACS analysis demonstrated no significant relative shift in the proportion of CD4(+) or CD8(+) cells. The adoptive transfer of CTDLN resulted in a significant reduction of pulmonary metastases as compared to TDLN from either tumor-bearing mice or mice who underwent surgical excision. Cryoablation prior to surgical resection of breast cancer can be used as a method to generate effector T-cells for adjuvant adoptive cellular immunotherapy.

Adenocarcinoma↗
PubMed2006

MACO: a gapped-alignment scoring tool for comparing transcription factor binding sites.

We have implemented a novel gapped-alignment algorithm to compare Position Frequency Matrices (PFMs) for Transcription Factor Binding Sites. The application compares an input PFM with those collected from public databases and outputs similarity scores, sequence alignments and related PFM clusters. MACO is freely accessible on a web server located at www.nicemice.cn/bioinfo/MACO. Source code is distributed upon request to the authors.

Algorithms↗
PubMed2005

Rational synthesis and magnetic properties of a family of low-dimensional heterometallic Cr-Mn complexes based on the versatile building block [Cr(2,2'-bipyridine)(CN)4]-.

Six heterometallic compounds based on the building block [Cr(bpy)(CN)4]- (bpy = 2,2'-bipyridine) with secondary and/or tertiary coligands as modulators, {Mn(H2O)2[Cr(bpy)(CN)4]2}n (1), {Mn(bpy)(H2O)[Cr(bpy)(CN)4]2 x H2O}n (2), [Mn(bpy)2][Cr(bpy)(CN)4]2 x 5H2O (3), {[Mn(dca)(bpy)(H2O)][Cr(bpy)(CN)4] x H2O}n (4) (dca = N(CN)2(-)), {Mn(N3)(CH3OH)[Cr(bpy)(CN)4] x 2H2O}n (5), and {Mn(bpy)(N3)(H2O)[Cr(bpy)(CN)4] x H2O}2 (6), have been prepared and characterized structurally and magnetically. X-ray crystallography reveals that the compounds 1, 2, 4, and 5 consist of one-dimensional (1D) chains with different structures: a 4,2-ribbon-like chain, a branched zigzag chain, a 2,2-CC zigzag chain, and a 3,3-ladder-like chain, respectively. It also reveals that compound 3 has a trinuclear [MnCr2] structure, and compound 6 has a tetranuclear [Mn2Cr2] square structure. Magnetic studies show antiferromagnetic interaction between Cr(III) and Mn(II) ions in all compounds. All of the chain compounds exhibit metamagnetic behaviors with different critical temperatures (Tc) and critical fields (Hc; at 1.8 K): 3.2 K and 3.0 kOe for 1; 2.3 K and 4.0 kOe for 2; 2.1 K and 1.0 kOe for 4; and 4.7 K and 5.0 kOe for 5, respectively. The noncentrosymmetric compound 2 is also a weak ferromagnet at low temperature because of spin canting. The magnetic analyses reveal Cr-Mn intermetallic magnetic exchange constants, J, of -4.7 to -9.4 cm(-1) (H = -JS(Cr) x S(Mn)). It is observed that the antiferromagnetic interaction through the Mn-N-C-Cr bridge increases as the Mn-N-C angle (theta) decreases to the range of 155-180 degrees, obeying an empirical relationship: J = -40 + 0.2theta. This result suggests that the best overlap between t(2g) (high-spin Mn(II)) and t(2g) (low-spin Cr(III)) occurs at an angle of approximately 155 degrees.

Journal Article↗
PubMed2005

Immunologic response to cryoablation of breast cancer.

PURPOSE: With improvements in breast imaging and image-guided interventions, there is interest in ablative techniques for breast cancer. Cryosurgery initiates inflammation and leaves tumor-specific antigens intact, which may induce an anti-tumor immune response. To help define the mechanisms involved in the cryoimmunologic response, we compared cryosurgery to surgery in a murine model of breast cancer. EXPERIMENTAL DESIGN: BALB/c mice with MT-901 tumors underwent cryoablation or resection. Mice successfully treated were re-challenged with MT-901 or RENCA. Serum cytokine levels were analyzed by ELISA. Tumor draining lymph nodes (TDLN) and spleens were harvested, lymphocytes were activated and assessed for a specific anti-tumor response by both an interferon-gamma (IFNgamma) release assay and ELISPOT. NK cell activity was assessed by cytotoxicity against YAC-1, an NK-susceptible cell line. RESULTS: After re-challenge, tumors developed in 86% of mice treated by surgical excision compared to 16% of mice treated by cryosurgery (p=0.025). Cryoablation of MT-901 had no effect on re-challenge with RENCA. Cryoablation led to significantly higher levels of interleukin (IL)-12 (383.6 pg/ml +/- 32.8 versus 251.6 +/- 16.5, p=0.025) and IFN-gamma (1564 pg/ml +/- 49 versus 1244 pg/ml +/- 101, p=0.009), but no changes in IL-4 or IL-10. Tumor-specific T-cell responses were evident after cryosurgery in lymphocytes from TDLN but not from spleen. Cryoablation also increased NK activity compared to surgery (24.5% +/- 17.3 versus 16.5% +/- 5.9, p < 0.001). CONCLUSION: Cryoablation results in the induction of both a tumor-specific T-cell response in the TDLN and increased systemic NK cell activity, which correlates with rejection of tumors upon re-challenge.

Animals↗
PubMed2004

Linking cyano-bridged ladders by azide to form a layered metamagnet.

Cyano-bridged corrugated ladder-like chains are connected by mu(1,1)-N(3) (end-on, EO) bridges to form a layered coordination polymer, [Mn(N(3))(CH(3)OH)[Cr(phen)(CN)(4)].CH(3)OH (2), which is the first mixed cyano-azide bridged compound, and shows metamagnetism below 21.8 K, a temperature much higher than that (3.4 K) of the crossed double chain [Cr(phen)(CN)(4)](2) [Mn(H(2)O)(2)].4H(2)O (1) based on the same building block [Cr(phen)(CN)(4)](-).

Journal Article↗
PubMed2003

Long-range ferromagnetic ordering in two-dimensional coordination polymers Co[N(CN)2]2(L) [L = pyrazine dioxide (pzdo) and 2-methyl pyrazine dioxide (mpdo)] with dual mu- and mu3-[N(CN)2] bridges.

Two novel coordination polymers of Co(II) with dicyanamide (dca) were obtained by the addition of ancillary ligands of pyrazine dioxide (pzdo) and 2-methyl pyrazine dioxide (mpdo) into the Co-dca binary system, respectively. Co[N(CN)(2)](2)(pzdo) (1) crystallizes in the orthorhombic space group of Pnnm (No. 58) with a = 9.4699(5) A, b = 14.9984(3) A, c = 7.4313(7) A, and Z = 4, while Co[N(CN)(2)](2)(mpdo) (2) is in the monoclinic space group C2 (No. 5) with a = 16.5391(4) A, b = 9.6065(2) A, c = 7.5001(2) A, beta = 105.779(1) degrees, and Z = 4. Both complexes contain similar two-dimensional triangular Co-dca layers, which offer rare examples of mixed 1,5-mu- and mu(3)-dca bridging coordination polymers with long-range ferromagnetic ordering below ca. 2.5 K.

Journal Article↗
PubMed2002

Local injection of M-CH combined with i.p. hyperthermic hypo-osmolar infusion is an effective therapy in advanced gastric cancer.

Treatment failure of surgically treated gastric cancer is attributed to the spread of gastric cancer cells into the abdominal cavity and lymphatic or hematogenic canals. In the present study, local injection of mitomycin C bound to activated carbon (M-CH) combined with i.p. hyperthermic hypo-osmolar infusion (IPHHOI) was intraoperatively administered to prevent lymph node recurrence and peritoneal recurrence of gastric cancer. Between April 1998 and August 1999, 79 patients with advanced gastric cancer were allocated randomly to two groups. Forty patients underwent M-CH plus IPHHOI combined with surgery (M-CH1+IPHHOI group) and the remaining 39 underwent surgery alone (control group). Lymph node and peritoneal recurrence were significantly decreased in the M-CH1+IPHHOI group compared to that in the control group (p<0.05). The 1- and 2-year survival rates for the M-CH1+IPHHOI group were 91.2 and 72.1%, and those for the control group were 78.9 and 45.5%. The M-CH1IPHHOI group reaped a significant survival benefit (p=0.0352) compared to the control group. Although this study was conducted randomly for a small number of patients and short time, compared with the control group, the M-CH1+IPHHOI group had a beneficial effect in preventing lymph node recurrence and peritoneal recurrence after curative gastrectomy for advanced gastric cancer.

Adenocarcinoma↗