Biomedical subjects

Gang Wang

Publications and source records attributed to Gang Wang.

214 records · Page 12Linked to original sources

PubMed2002

[Operative treatment of acetebular fractures].

OBJECTIVE: To discuss the diagnosis and operative technology of acetabular fracture. METHODS: 41 cases of displaced acetabular fractures were enrolled. CT and 3D-CT were performed before operation to understand the classification of fractures and to determine operative regimen. All cases were fixed by reconstruction plates and screws through the best approach. RESULTS: 39 cases were followed up for 1 - 4 years, with an average of 1.5 years. Curative outcomes were assessed by Matta's criteria. Clinically, 16 (53.3%) 30 fresh fractures were excellent, 11 (36.7%) good, 3 (10%) fair. The rate of excellence and goodness was 90%. In 9 cases of old fractures, 2 were excellent, 3 good, 2 fair and 2 poor. The rate of excellence and goodness was 55.6%. Complications consisted of sciatic never injury, necrosis of the femoral head, and heterotopic ossification. CONCLUSIONS: The time of operation, degree of injury, and quality of reduction of fracture are important factors affecting the postoperative function of the hip. The key to enhance the outcome is to verdict the type of fracture, select the proper approach before operation, and precise reduction and appropriate internal fixation during operation.

Acetabulum↗

PubMed2002

[Spatial pattern of Tetranychus urticae population in apple tree garden].

Spatial pattern of insect population is an important aspect of insect ecology. It reveals the spatial structure characteristics of populations, and provides a basis for relevant sampling techniques and data transformation. Tetranychus urticae Koch is a serious pest of apple tree. Its spatial pattern and time-series dynamics were analyzed by calculating four indices of aggregation, and parameters of Iwao model. The results showed that the spatial pattern of T. urticae in apple tree garden belonged to an aggregated distribution pattern. The aggregation intensity was the highest in upper and southern part of crown, and the lowest in its lower and inner part. The aggregation was due to the biological habits of T. urticae and environmental heterogeneity. No matter T. urticae was in the upper, middle, or lower crown of apple tree, its aggregation intensity was higher before first ten days of June, and then gradually decreased after the middle ten days of June.

Acari↗

PubMed2002

[Application of fuzzy cluster in analyzing population dynamics of Chorthippus fallax].

The population dynamics includes quantity dynamics, space dynamics and temporal dynamics. They are interdependent and mutually influential. Fuzzy cluster analysis was carried out with the data of mean densities, indices of patchiness and occupied plot rates of Chorthippus fallax(Zubovsky) on Ganjia High Mountain Grassland in Xia-he County, Gansu Province. The results showed that the population dynamics of this grasshopper could be divided into four distinct periods, i.e., the initial stage of hatching and the last stage of adult, the peak stage of nymph, the initial stage of adult, and the peak stage of first nymph and the adult. The peak period of nymph was in the middle ten days to the last ten days of July. In this period, the eggs were completely hatched, the population was mainly composed of nymphs of 2 and 3 instars, and the population size was related to the numbers and harmful levels of this grasshopper in the same year. Therefore, this period was the key time for population prediction and chemical control.

Animals↗

PubMed2002

[Mechanism and universality of Yoda's -3/2 law of self-thinning in plant population].

According to plant modular theory, the Yoda's-3/2 law of self-thinning was theoretically rederived in this paper. With the parameters in the expression of Yoda's-3/2 law being given biological meanings, this article offered universal and rational explanations for relevant data in literatures that could not be explained before. Based on the authors' hypothesis, the experiments on the self-thinning process of spring wheat (Triticum aestivum L.) were conducted. Individual morphological and population quantitative indexes were examined at regular intervals. The results showed that the self-thinning process of spring wheat conformed to Yoda's-3/2 law of self-thinning. At the same time, the universality of Yoda's-3/2 law of self-thinning was explained theoretically by analyzing individual morphological data.

Elasticity↗

PubMed2002

[Application of SPOT 4 remote sensing imagine in classification of Qira oasis landscape in China].

The landscape surounding Qira osais at the southern fringe of the Taklamakan Desert in China was analyzed by using the SPOT 4 multispectrum remote sensing data and GPS (global positioning system) data. The SPOT 4 scene was projected into an UTM grid, and a supervised classification by ERDAS IMAGINE software was applied. In total, 13 landscape units could be distinguished, and a "frame of reference" was set up for establishing monitoring system of landscape patterns dynamics. Limitations, possible improvements and further applications of the approach were discussed.

China↗

PubMed2002

[The value of specific neuronal anti-Hu antibody in the early diagnosis of small cell lung cancer].

OBJECTIVE: To evaluate the value of specific neuronal anti-Hu antibody in the early diagnosis of small cell lung cancer (SCLC). METHODS: Ninety-eight patients with SCLC were included in the study. Specific neuronal anti-Hu antibody was detected by immunohistochemistry (the ABC method) and Western blot. RESULTS: The sera from 40% (32/80) of the patients in group A (without paraneoplastic syndrome of the nervous system) reacted with the frozen sections of human cerebra and were stained by indirect immunoperoxidase method at final dilutions of 1:1,000 to 1:8,000, while the sera from 89% of the patients (16/18) in group B (with paraneoplastic syndrome of the nervous system) reacted at final dilutions of 1:1,000 to 1:64,000. Western blot analysis revealed that the specific neuronal anti-Hu antibody was identified at several bands of approximately 35,000 approximately 40,000 with nuclear extracts, and at 40,000 with HuD clonal protein. The anti-Hu antibody was detected in only two patients from group A, but in 16 patients from group B. The antibody was not detected in sera from 32 normal human subjects. CONCLUSIONS: Specific neuronal anti-Hu antibody at high titers was detected in patients with SCLC not complicated with paraneoplastic syndrome of the nervous system. This antibody may be useful in the early diagnosis of SCLC.

Adult↗

PubMed2002

[Screening and cloning gene of hepatocyte protein interacting with hepatitis C virus core protein].

OBJECTIVE: To clone the unknown gene of hepatocyte protein interacting with hepatitis C virus core protein. METHODS: Using the yeast dual hybrid system 3, bait plasmids of hepatitis C virus core were constructed. After identifying hepatitis C virus core protein that could stably expressed in AH109 yeast strains, we performed yeast two hybrid by mating AH109 with Y187 that transformed with liver cDNA library plasmids pACT2 and then plated on quadrople dropout (QDO) medium and assayed for alpha-gal activity. The genes of yeast colonies that could grow on QDO and had alpha-gal activity were sequenced. RESULTS: Among the 30 positive colonies, we blasted the gene of the sixth colony; we coined human hepatitis C virus binding protein 6(Hu Hcbp6) with Genbank, realized that the Hu Hcbp6 shares as much as 98% homology with two cDNA without knowing functions. We have proved that Hu Hcbp6 could interact with hepatitis C virus core protein. CONCLUSIONS: Hepatitis C virus core binding protein (Hu Hcbp 6 Genbank number: AY032594) was successfully cloned and identified. The study partly paved the way for investigating physiological function of the Hu Hcbp6.

Cloning, Molecular↗

PubMed2002

[Screening and application of human-derived HBsAg bacteriophage single chain antibody in clinical diagnosis].

OBJECTIVE: To identify human single chain Fv antibody (ScFv) against hepatitis B viral surface antigen. METHODS: The recombinant phages were panned by HBsAg which was coated in a microtiter plate, after five rounds of biopanning, 56 phage clones were identified specific to HBsAg. The specificity of ScFv was evaluated by ELISA and immunohistochemistry, respectively. RESULTS: The data of HB sAg-ScFv DNA digestion and DNA sequencing showed that the ScFv gene is composed of 750 bp. ELISA and immunohistochemistry demonstrated that the human single chain Fv antibody against hepatitis B surface antigen has a specific combination character with hepatitis B surface antigen of different sources and paraffin-embedded patients tissue specimens, it did not react with normal liver tissue and HCV. CONCLUSIONS: The application of HBsAg specific ScFv in immunohistochemistry was successfully achieved.

Bacteriophages↗

PubMed2002

[Quasispecies and variations of hepatitis B virus: core promotor region as an example].

OBJECTIVE: To investigate the HBV quasispecies groups in the patients with chronic HBV infection. METHODS: A set of specific primers was synthesized according to DNA sequence of HBV strain found in China. The whole core promoter (CP) region was amplified by PCR method from the sera of 3 patients with chronic HBV infection, and the PCR products were subcloned into pGEM Teasy vectors. The clones were randomly selected to be sequenced. Sequence comparison of the selected clones was made to find the difference. RESULTS: By comparison, it was found that each sequence of selected clones was different. The point mutation always occurred in TATA-like boxes, especially from T to C replacement on 184 site. There is a hot region (33.3% 5/15) in basic core promoter where deletion mutation frequently happened. CONCLUSIONS: There is a hot deletion region near DR I in CP. The replacement at 184 nt (T to C) in the third TATA-like box may influence the expression of pre-C/C protein. The sequencing results suggest that there are HBV quasispecies groups in chronically infected patients.

DNA, Viral↗

PubMed2002

Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat.

OBJECTIVE: To search for genomic DNA sequence of the augmenter of liver regeneration (ALR) of rat. METHODS: Polymerase chain reaction (PCR) with specific primers was used to amplify the sequence from the rat genome. RESULTS: A piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified. The lengths of the gene and pseudogene are 1508 bp and 442 bp, respectively. The ALR gene of rat includes 3 exons and 2 introns. The 442 bp DNA sequence may represent a pseudogene or a ALR-related peptide. Predicted amino acid sequence analysis showed that there were 14 different amino acid residues between the gene and pseudogene. ALR-related peptide is 84 amino acid residues in length and relates closely to ALR protein. CONCLUSION: There might be a multigene family of ALR in rat.

Amino Acid Sequence↗

PubMed2002

Cloning and expression of the gene of augmenter of liver regeneration in yeast cells.

OBJECTIVE: To study the function of augmenter of liver regeneration (ALR) as a regulatory factor that specifically stimulates hepatic cell regeneration, we constructed yeast expressive vector of ALR and expressed it in yeast cells. METHODS: Total RNA was extracted from HepG2 cells, and reverse transcription polymerase chain reaction (RT-PCR) was performed to amplify the coding region of ALR. The products were cloned into PGEM-T vector and sequenced, then cloned into PGBK T7 vector. The recombinant plasmid PGBK T7-ALR was transformed into yeast AH109. The yeast protein was extracted and analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting hybridization technique. RESULTS: DNA sequencing results confirmed that the coding region of ALR was correctly inserted into the yeast expression vector, and Western blotting assay showed that recombinant ALR was successfully expressed in yeast. Its molecular weight was identical to the theoretical value of 15 000 Da; the protein was found inside the yeast cells. CONCLUSION: The successful expression of ALR in yeast cells makes it possible to study further on its biological function.

Blotting, Western↗

PubMed2002

[Transfer of insecticidal protein gene from Bacillus thuringiensis into conventional maize inbred-line mediated by Agrobacterium tumefaciens].

Excellent inbred-lines of maize,340 and 4112, which were used largely in hybridized combination were transformed with Agrobacterium tumefaciens. The immature embryos and their original calli were infected by A.tumefaciens LBA4404 containing plasmid pGBIL04. After 3 days of co-cultivation, the immature embryos and calli were continuously selected on the medium containing phosphinothricin (PPT) for 3 generations, then plants were regenerated. It was proved by PCR analysis that the target Bt gene had been integrated into the genome of regenerated plants. The results showed that fresh original calli from the immature embryos after pre-culture were suitable acceptors. The results also showed that it could increase the frequency of selection by properly lowering the co-culture temperature to 22 degrees .

English Abstract↗

PubMed2002

[The studies of sensitivity of genotypes in soybean to lines of Agrobacterium tumefaciens].

The sensitivity of genotypes in soybean to lines of Agrobacterium tumefaciens and the ability of A.tumefaciens infecting to soybean were investigated with hypocotyls of soybean (Jilin30, Jilin43 ,Suinong8, Heinong35 and Dongnong42) and lines of A. tumefaciens LBA4404 and EHA105 which including plasmid pGBI121S4ABC and pGBI4A2B respectively. The results showed that the sensitivity of genotypes in soybean to A. tumefaciens was significantly different. Jilin43 was the most sensitive materials to A. tumefaciens. The ability of A. tumefaciens infecting hypocotyls in soybean was different. LBA4404 including plasmid pGBI121S4ABC was easier to infect hypocotyls of soybean.

English Abstract↗

PubMed2000

Expression of LOX-1, an oxidized low-density lipoprotein receptor, in experimental hypertensive glomerulosclerosis.

Oxidized low-density lipoprotein (OxLDL) has been implicated in atherosclerosis and glomerulosclerosis. LOX-1 is a recently identified OxLDL receptor that is abundantly expressed in vascular endothelial cells. The aim of the present study was to investigate LOX-1 expression in the kidneys of hypertensive rats. Dahl salt-sensitive (DS) and salt-resistant (DR) rats were fed a 0.3% or 8% NaCl diet. Some DS 8% rats were treated with manidipine or hydralazine. LOX-1 gene expression was markedly elevated in the kidneys and glomeruli of hypertensive DS 8% rats compared with those of normotensive DR and DS 0.3% rats. Prolonged salt loading further increased the renal LOX-1 expression in DS rats. The LOX-1 upregulation in DS 8% rats was accompanied by renal overexpression of transforming growth factor-beta 1 and type I collagen, impaired renal function, and histologic glomerulosclerotic changes, all of which were ameliorated by antihypertensive treatment. LOX-1 was indeed expressed in the glomeruli in vivo and in cultured glomerular cells in vitro. However, LOX-1 expression was elevated in the aorta but not the kidneys of spontaneously hypertensive rats, which exhibited hypertension but minor glomerulosclerotic changes. In conclusion, the LOX-1 upregulation in the kidney of DS 8% rats was parallel to glomerulosclerotic changes and renal dysfunction, suggesting a possible pathogenetic role for renal LOX-1 in the progression to hypertensive glomerulosclerosis.

Animals↗

PubMedDate not supplied

Selection and identification of human gonadotropin-releasing hormone promoter binding peptides by phage display-CEMSA.

Specific interactions between transcription factors and cis-acting DNA sequences form the molecular basis of gene expression regulation. Here, we applied phage display technology to DNA-protein interaction studies. A phage-displayed peptide library was used to select Gonadotropin-releasing hormone promoter (GP) binding peptides. After four sequential rounds of biopanning on GP-conjugated magnetic beads, phage clones encoding GQPTPRNAGLPL (B6), SRLNVEPLTTYS (B3), and TTLHWASLTTGR (B11) were enriched. Phages bearing these peptides showed specific binding to GP in solution by capillary electrophoresis mobility shift assay (CEMSA). In addition, some human transcription factors were speculated as the potential transcription factors or co-activators of GnRH gene by bioinformatic analysis. These results suggest that phage display-CEMSA methodology should be a powerful tool to screen and identify site-specific DNA-binding peptides.

Amino Acid Sequence↗

PubMedDate not supplied

Clinical analysis of 48 cases of inverse psoriasis: a hospital-based study.

Inverse psoriasis, rare in clinical practice, refers to psoriasis only or mainly occurring at flexural sites, such as the axilla, antecubital fossae, popliteal fossae, and inguinal creases. It is also known as flexural psoriasis. With a total collection of psoriatic cases from September 2002 to December 2003 at Xijing hospital, we made a retrospective analysis of the disease history, clinical characteristics, and treatment of the patients affected with inverse psoriasis. The results showed that the major clinical manifestations of inverse psoriasis were sharply demarcated erythematous plaques with varying degrees of infiltration and minimal or no scales. Affected areas often involve the groin, axilla, genitals, and umbilicus. The disease responds well to the narrow band UVB therapy. Compared with common psoriasis, inverse psoriasis has similar and unique characteristics in terms of the affected areas, clinical symptoms, and responses to the treatment.

Adolescent↗