Using beta-lactamase to trigger supramolecular hydrogelation.
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Catalysis↗
Biomedical subjects
Gaolin Liang
Publications and source records attributed to Gaolin Liang.
Using enzymatic reactions to enhance the photodynamic therapy effect of porphyrin dityrosine phosphates.
This paper reports the synthesis and photodynamic therapy (PDT) effect of a porphyrin derivative containing tyrosine phosphate, which promises a new, useful approach to develop PDT agents.
Cell Survival↗
Using a kinase/phosphatase switch to regulate a supramolecular hydrogel and forming the supramolecular hydrogel in vivo.
We have designed and synthesized a new hydrogelator Nap-FFGEY (1), which forms a supramolecular hydrogel. A kinase/phosphatase switch is used to control the phosphorylation and dephosphorylation of the hydrogelator and to regulate the formation of supramolecular hydrogels. Adding a kinase to the hydrogel induces a gel-sol phase transition in the presence of adenosine triphosphates (ATP) because the tyrosine residue is converted into tyrosine phosphate by the kinase to give a more hydrophilic molecule of Nap-FFGEY-P(O)(OH)(2) (2); treating the resulting solution with a phosphatase transforms 2 back to 1 and restores the hydrogel. Electron micrographs of the hydrogels indicate that 1 self-assembles into nanofibers. Subcutaneous injection of 2 in mice shows that 80.5 +/- 1.2% of 2 turns into 1 and results in the formation of the supramolecular hydrogel of 1 in vivo. This simple biomimetic approach for regulating the states of supramolecular hydrogels promises a new way to design and construct biomaterials.
Adenosine Triphosphate↗
Supramolecular hydrogels based on beta-amino acid derivatives.
Here we report on a new class of supramolecular hydrogels based on dipeptides that consist of beta-amino acids, which may confer proteolytic resistance to the hydrogels for biomedical applications.
Journal Article↗
Formation of a new oxidative metabolite from kobophenol A by human intestinal bacterium Klebsiella pneumoniae.
During our studies on the metabolism of kobophenol A (1) in rats, we had isolated, purified, and identified the main new oxidative metabolite of 1, koboquinone A (2) from rats' feces. To elucidate the metabolic pathway of 1 in rats, we conducted the in vitro metabolic experiments of 1 by human intestinal bacteria and found that Klebsiella pneumoniae produced appreciable amounts of 2. This was verified by means of high performance liquid chromatography mass/mass spectrometry (HPLC/MS/MS) analysis.
Benzoquinones↗
X-ray single-crystal analysis of (-)-(S)-equol isolated from rat's feces.
The biological effects of soy isoflavones have attracted considerable interest in recent years, leading to numerous studies on dietary intake and epidemiology. (-)-(S)-equol (3) is a metabolite produced in vivo from the isoflavone daidzein, a kind of soy phytoestrogen, by the action of gut microflora. It has higher biological activity than its precursor. Here, we wish to report the isolation and first X-ray crystal structure of 3 from the feces of rats fed a soy-isoflavone-containing diet.
Animals↗
Koboquinone A and B, new metabolites of kobophenol a in rats.
Two new phase I metabolites of phytoestrogen kobophenol A (1), called koboquinone A (2) and B (3), have been isolated from the feces of rats orally administered 1. Their structures were determined by spectroscopic methods. 2 also showed activity of stimulating the proliferation of cultured osteoblasts.
Animals↗