Biomedical subjects

Gary L Hook

Publications and source records attributed to Gary L Hook.

3 recordsLinked to original sources

PubMed2004

Dynamic solid phase microextraction for sampling of airborne sarin with gas chromatography-mass spectrometry for rapid field detection and quantification.

A portable dynamic air sampler and solid phase microextraction were used to simultaneously detect, identify, and quantify airborne sarin with immediate analysis of samples using a field portable gas chromatography-mass spectrometry system. A mathematical model was used with knowledge of the mass of sarin trapped, linear air velocity past the exposed sampling fiber, and sample duration allowing calculation of concentration estimates. For organizations with suitable field portable instrumentation, these methods are potentially useful for rapid onsite detection and quantification of high concern analytes, either through direct environmental sampling or through sampling of air collected in bags.

Air↗

PubMed2003

Detection of VX contamination in soil through solid-phase microextraction sampling and gas chromatography/mass spectrometry of the VX degradation product bis(diisopropylaminoethyl)disulfide.

A solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) sampling and analysis method was developed for bis(diisopropylaminoethyl)disulfide (a degradation product of the nerve agent VX) in soil. A 30-min sampling time with a polydimethylsiloxane-coated fiber and high temperature alkaline hydrolysis allowed detection with 1.0 microg of VX spiked per g of agricultural soil. The method was successfully used in the field with portable GC-MS instrumentation. This method is relatively rapid (less than 1 h), avoids the use of complex preparation steps, and enhances analyst safety through limited use of solvents and decontamination of the soil before sampling.

Chemical Warfare Agents↗

PubMed2002

Application of headspace solid-phase microextraction and gas chromatography-mass spectrometry for detection of the chemical warfare agent bis(2-chloroethyl) sulfide in soil.

A field expedient analytical method for detecting the chemical warfare agent (CWA) sulfur mustard as a soil contaminant was developed using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS). Five commercially available SPME fibers were investigated to determine the optimal fiber, and extraction conditions. Polyacrylate and carbowax-divinylbenzene fiber coatings gave a statistically indistinguishable and best response compared to the other three types examined in a simple system studied without soil. The polyacrylate fiber coating was selected for study of a system in which sulfur mustard was spiked to an agricultural soil (Standard Reference Material 2709, San Joaquin type). With soil samples, the greatest sensitivity occurred by the addition of deionized water to spiked soil and extraction at ambient temperature for 20 min or longer. SPME sampling with GC-MS analyses afforded good reproducibility (relative standard deviation between 2 and 10%), and analyte concentrations as low as 237 ng/g were detected in soil (total ion chromatograms). As completed here, total time for sampling and analysis was just under 1 h, and use of organic solvents or special sample introduction equipment was avoided.

Chemical Warfare Agents↗