Biomedical subjects

Gerald Mingin

Publications and source records attributed to Gerald Mingin.

3 recordsLinked to original sources

PubMed2006

Global gene expression patterns in mouse wolffian duct development.

PURPOSE: We identified genes responsible for terminal differentiation of the mouse ureter. MATERIALS AND METHODS: We isolated the wolffian ducts of Black Swiss mice from embryonic days 12.5 to 14.5. These ducts were processed for total RNA extraction. RNA was amplified and converted to biotinylated cDNA, which was hybridized to Affymetrix(R) mouse 430 microarray GeneChips. Data were analyzed using Affymetrix software provided by the core facility at our institution. Data were confirmed by semiquantitative polymerase chain reaction and immunohistochemistry. RESULTS: Of 42,000 evaluable transcripts 412 were up-regulated and 133 were down-regulated more than 1.5-fold between embryonic days 12.5 and 14.5. Of up-regulated genes 17 were up-regulated more than 3-fold. Of these genes 5 were selected for further study and they were identified as having roles in cell growth/regulation in general. Increased expression of Foxa1 was seen at embryonic day 14.5 in the distal ureter and urogenital sinus. CONCLUSIONS: To our knowledge this is the first report of differential gene expression patterns in the developing mouse wolffian duct. Our results diverge from expression patterns reported in the kidney. This information will enable future comparisons between WT and transgenic mice with an abnormal phenotype.

Animals↗

PubMed2004

Linear growth after enterocystoplasty in children and adolescents: a review.

The interposition of bowel in continuity with the urinary tract has allowed for the preservation of renal function and continence in children with bladder exstrophy, as well as neurogenic and valve bladders. Although bladder augmentation with ileum or colon has been shown to be safe, the long-term effects of metabolic acidosis in addition to abnormalities in linear growth and bone metabolism remain largely unknown. We reviewed the literature to critically examine linear growth in children who have had bladder augmentation with a particular emphasis on the correlation between acid-base status, bone mineralization and growth. The majority of studies suggest that linear growth is not affected by bladder augmentation. In the short-term, children post-augmentation have varying degrees of metabolic acidosis which, overtime, appears to resolve with no affect on linear growth. In a single study, bladder augmentation led to significant bone demineralization almost a decade after surgery, however, even in these children no decrease in linear growth was noted. No alterations in bone density levels were seen with short-term follow-up.

Acid-Base Equilibrium↗

PubMed2002

Management of chordee in children and young adults.

Penile curvature is a spectrum of disease affecting boys with and without hypospadias. The etiology of chordee includes skin tethering, fibrotic bucks or dartos fascia, corporeal body disproportion and rarely a fibrotic urethra. Several surgical techniques (plication, excision, and graft insertion) are currently employed to repair penile curvature. Recent neuroanatomical studies of the developing fetal penis have shown that the dorsal nerve branches from the 11 and 1 o'clock positions to the 5 and 7 o'clock positions, being absent in the midline. Since the neuroanatomy is similar in both the hypospadiac and normal penis, we now recommend performing penile straightening in both hypospadiac and non hypospadiac patients with significant curvature by the placement of plication sutures at the 12 o'clock position. Placement of dorsal midline plication sutures corrects curvature without risk to the underlying nerve structures.

Adult↗