Biomedical subjects

Grazyna Pyka-Fościak

Publications and source records attributed to Grazyna Pyka-Fościak.

3 recordsLinked to original sources

PubMed2006

Erythrocyte stiffness in diabetes mellitus studied with atomic force microscope.

The mechanical properties of erythrocyte membrane have been studied using an atomic force microscope. Measurements were carried out on blood samples taken from 7 diabetes mellitus patients and 8 healthy individuals. For each blood sample a distribution of a Young's modulus was constructed. It has been found that both the mean value and the width of the distribution in diabetic patients exceed the corresponding results for healthy persons by a factor greater than 3. The high sensitivity of the atomic force microscopy and the ability to measure the full distribution of the erythrocyte membrane Young's modulus makes it a unique, powerful and promising tool in studies of the membrane stiffness of red blood cells.

Diabetes Mellitus↗

PubMed2005

Erythrocyte stiffness probed using atomic force microscope.

The stiffness of erythrocytes in patients (N=45) suffering from certain disorders, such as coronary disease, hypertension, and diabetes mellitus has been assessed using the Atomic Force Microscopy (AFM) and compared with that in a group of healthy individuals (N=13). For each blood sample, around 20 erythrocytes were selected at random and the stiffness of each one was probed in 20-30 arbitrarily chosen points. From these results, distributions of the cell Young's modulus (YM) were determined. Average values and widths of YM distributions significantly increased in samples taken from diabetes mellitus patients and cigarette smokers, as compared to those taken from healthy donors. At the same time, the average values of YM were found to increase as a function of the patient's age. We demonstrated that the atomic force microscope is a very sensitive tool for determination of cell stiffness with every prospect of a routine application as a diagnostic tool in quantitative analysis of the physiological and pathological states of red blood cells.

Adolescent↗

PubMed2003

Ultrastructural studies on the formation of egg envelopes in fulgoromorphans (Insecta, Hemiptera, Fulgoromorpha: Dictyopharidae).

Follicular cells in ecuadorian dictyopharids diversify into two subpopulations: the main body cells (MFs) and cells surrounding the anterior pole of the oocyte (AFs). The synthetic activity of both categories of follicular cells is manifested by the presence of numerous cisternae of rough endoplasmic reticulum, Golgi complexes and vacuoles containing electron-dense material in their cytoplasm. The MFs synthesize precursors of the main body chorion, whereas the AFs are responsible for the formation of micropylar apparatus and respiratory tubules. The main body chorion is composed of thin endochorion and exochorion which forms pillar-like projections.

Animals↗